TnAs2

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 8654 bp

Ends


IR Length : 37/38

IRL : GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAGGCATAGCCGACC
IRR : GGGGTCGCCTCAGAAAACGGAAAATAAAGCACGCTAAGCCGGTTGCAGCG

Insertion site


Left flankDirect repeatRight flankDR Length
CTGGCGGATCTGCATGGCCGACGGC5

DNA sequence

GGGGTCGTCTCAGAAAACGGAAAATAAAGCACGCTAAGGCATAGCCGACCCTGCCAGGCTTGTTCCGCCCTGTAGCGACGCGATCAGCGGGCAGGAAACA
TTCCCCTTCCGCGCATGGCAGGCGCACACCAGTTCAGACAGCACGGTTTCCATGCGCGCCAGGTCGGCCATCTTTTCGCGCACGTTCTGAAGCTTGTGCT
CGGCCAGGCTGCTGGCTTCCTCGCAGTGGGTGCCGTCGTCGAGCCGCAGCAGATCGGCTATTTCATCCAGGCTGAAGCCCAGCCGCTGGGCCGATTTCAC
GAATCGCACTCGCGTCACATCCGCCGCGCCATAGCGGCGAATGCTGCCGTAAGGCTTATCCGGTTCCGGCAGCAAGCCCTTACGCTGATAGAAGCGGATC
GTTTCCACATTGACGCCGGCCGCCTTGGCAAAAACGCCAATGGTCAGGTTCTCTAAATTGTTTTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTA
AGGTTACGCTATCCAATTTAAATCCAAAAGGACAAGCGCATGTCTGAACCACAAACTGGGCGCGGCGCGCTCTTCGCCGGCGGGCTGGCCGCCATCCTCG
CCTCCGCTTGCTGCCTCGGGCCGCTGGTTCTGATCGCCTTGGGCTTCAGCGGGGCTTGGATCGGCAACCTGACCGTGCTGGAGCCGTATCGCCCGATTTT
CATCGGCGCAGCGCTGGTGGCGTTGTTCTTCGCCTGGCGGCGCATCTACCGCCCTGCGCAAGCCTGTAGGCCGGGTGAGGTCTGCGCGATTCCCCAAGTG
CGAGCGACCTACAAGCTCATTTTCTGGGTCGTGGCCGTACTGGTTCTGGTCGCGCTCGGATTTCCCTATGTCATGCCATTTTTCTACTGATCGGAGTTCA
CCATGAAGAAACTGTTTGCCTCCCTCGCCCTCGCCGCCGTTGTTGCCCCCGTGTGGGCCGCTACCCAGACCGTCACGCTGTCCGTACCGGGCATGACGTG
CTCGGCCTGTCCGATCACCGTCAAGAAGGCGATTTCCAAGGTCGATGGCGTCAGTAAAGTTGCCGTGACCTTCGAGACCCGCGAAGCGGTGGTCACCTTC
GATGATGCCAAGACCAGTGTGCAGAAGCTGACCAAGGCAACCGGGGACGCGGGCTATCCCTCCAGCGTCAAGCAGTGAGCCACTGAACCCGACCCTGGGG
CGATCATGGGACTGATCACACGCATTGCCGACAAGGCCGGCGCGCTTGGCAGTGTGGTTTCCGCGATGGGCTGCGCCGCCTGTTTTCCGGCCATCGCCAG
CTTGGGCGCGGCCATCGGGCTGGGCTTTCTACAGGAATACGAAGGCTTGTTCATTTTCACGCTGCTGCCGCTGTTCGCCGTCGTCGCTTTGCTGGCGAAT
GCACTGGGCTGGCTCAGTCATCGGCAATGGCACCGCAGCCTGCTCGGCATGATCGGGCCAGCCATCGTGTTCGCCGGAACCGTCTGGTTGCTTGGCAACT
GGTGGACAGCGCGCCTCGTATACACCGGCCTGGCCCTGATGATCGGCGTGTCGATCTGGGACTTGGTTTCACCGGCAAATCGCCGCTGCGGGCCGGATGG
CTGCGAACTCCCAGCAAAACACGGCTGACGGCGTGAGCCAGCGGCCAACACAGAAAAGGAACGATGAATGACCACCCTCAAAATCACCGGCATGACTTGC
GACTCGTGCGCAGTGCATGTCAAGGAAGCCCTGGAGAAAGTGCCAGGCGTGCAGTCGGCGAATGTCTCATATACCAAGGGCAGCGCCAAGCTCGCCGTCG
AGACCGGCACATCGCCGGACGCGTTGACTGCCGCCGTGGCCGGACTCGGTTATCGGGCCACGCTCGCCGACGCGCTCGTGCCTCCGGTGGGCGGCGGCTT
GCTCGTCAAGATGCGCGAATGGCTAGGTAGCGGCGACAAGGCTGGTGATGATGGTGGCGGATTGCATATTGCCGTTATCGGCAGCGGCGGCGCGGCGATG
GCGGCGGCGCTGAAGGCCGTCGAGCAAGGCGCGCACGTCACCCTGATCGAGCGCGGCACTATTGGCGGCACCTGCGTCAATATCGGCTGCGTGCCGTCCA
AGATCATGATCCGCGCTGCTCACATCGCCCACTTGCGTCGAGAAAGCCCGTTCGATGGCGGCATGCCGCCCACACCGCCGACGATCTTGCGCGAGCGGCT
GCTGGCCCAGCAGCAGGCGCGCGTCGATGAACTGCGCCACGCCAAATACGAAGGCATCCTGGATGACAATCCCGCCATCTCCGTGCTGCACGGCGAAGCC
CGTTTCAAGGACGGCCACAGCCTGACCGTGCAGCTCAATGGCGGCGGCGAGCGTGTGGTGACTTTCGACCGTTGCCTGATCGCCACCGGCGCGAGTCCGG
CCGTGCCGCCGATCCCCGGCCTGAAAGACACACCCTACTGGACTTCCACCGAAGCGCTGGTCAGCGACACCATTCCCGAGCGGTTGGCGGTGATCGGCTC
ATCGGTGGTGGCGCTGGAACTGGCGCAAGCTTTTGCCCGGCTGGGCAGCCAGGTCACGATCCTGGCGCGCAGCACCTTGTTCTTCCGAGAAGACCCGGCC
ATCGGCGAGGCAATCACGGCGGCGTTCCGCGCCGAAGGGATCGAGGTGCTGGAGCACACCCAGGCCAGCCAGGTCGCGCACGAGGGCGGCGAATTCGTGC
TCACCACGGCGCACGGCGAGCTGCGTGCCGACAAACTGCTGGTCGCCACCGGTCGCTCGCCCAACACGCGCAGCCTGGCGCTGGACGCGGCAGGCGTCGC
GCTCAACCTGCAAGGCGCTATCGTCATCGACGTCGGCATGCGGACCAGCACGCCGGACATCTACGCGGCCGGCGACTGCACCGACCAGCCTCAGTTCGTC
TATGTGGCGGCAGCGGCCGGCACCCGTGCGGCGATCAACATGACGGGCGGTGACGCGGCGCTTAACCTTGCCGCCATGCCTGCCGTGGTGTTCACCGATC
CGCAAGTCGCCACCGTGGGCTACAGCGAGGCAGAGGCCCAGCACGACGGGATCGAGACCGACAGTCGCACCTTGACCTTGGACAACGTGCCGCGTGCGCT
CGCCAACTTCGACACACGCGGCTTCATCAAGCTGGTCATCGAGGAAGGTAGTGGACGGCTCATCGGCGTACAGGCGGTCGCGCCGGAAGCCGGCGAACTG
ATCCAGACGGCGGTACTCGCCATCCGTAACCGGATGACCGTGCAGGAACTGGCCGACCAGTTGTTCCCCTATCTGACGATGGTCGAGGGACTGAAGCTCG
CGGCGCAGACCTTTAACAAGGATGTGAAGCAGCTTTCCTGCTGCGCCGGGTGAGAAAAAGGAGGTGTTCGATGAACGCCTACACGGTGTCCCGGCTGGCC
CTTAATGCCGGGGTGAGCGTGCACATCGTGCGCGACTACCTGCTGCGCGGACTGCTGCGGCCGGTGGCGTGCACCCCTGGCGGCTACGGCCTGTTCGACG
ATGCGGCCTTGCAACGGCTGTGCTTCGTGCGGGCTGCTTTCGAAGCGGGTATCGGTCTCGACGCGCTGGCGCGGCTGTGCCGGGCGTTGGATACCGCGGA
CGGCGACGAAGCGGCCGCGCAGCTTGCCGTGCTGCGCCAGTTCGTCGAACGTCGGCGCGAAGCATTGGCCGATCTGGAGGTGCAGTTGGCCACCATGCCG
ACCGAGCTGGTACAGCATGCGGAGAGTCTGCCATGAACAGCCCCGAGCGCTTGCCGACCGAGACACACAAGCCGTTCACCGGCTACCTGTGGGGCGCGTT
GGCCGTGCTCACCTGTCCCTGCCATTTGCCGATTCTCGCTGTGGTGCTCGCGGGGACGACGGCCGGCGCGTTCATCGGGGAGCATTGGGGTATTGCAGCC
ATCACGCTGACCGGCTTGTTTGTCCTGTCCGTGACGCGGCTGTTGCGGGTCTTCAAGGAAAGATCGTGAGCACATCACTGCCCGCCAGATGGACGGCGAC
CGAATTGGCGCAGGCGGTTGTGCGCGGGCAGTTTGAGCTGCACTACCAGCCGATTGTCGATTTGCGCAGTGACCAGATTGTCGGTGCGGAAGCCCTGTTG
CGCTGGCGTCATCCGCAGCTCGGACTTTTGCCGCCGGGCCAGTTCCTGCCCGTGGTCGAATCGTCCGGCTTGATGCCTGAAATCGGCGCTTGGGTGCTGG
GCACCGCCTGCCGCCAGATGCGCGAATGGCGGATGCTGGCATGGCAACCGTTCCGGCTCGCCGTCAATGTTTCGGCGAGCCAGGTAGGACCGGATTTCGA
CGGGTGGGTAAAGGGCGTGCTGGCTGACGCCGAGTTGCCTGCCGAGTATCTGGAAATTGAGCTGACCGAATCGGTCGCGTTCGGCAATCCGGCGATCTTC
CCAGCCCTGGAAGCCTTGCGACAGATCGGTGTGCGCTTCGCTGCCGATGACTTCGGGACGGGGTATTCCTGTCTGCAACACCTGAAGTGCTGCCCGATTA
CTAAGCTCAAGATCGACCAGTCCTTCGTCGCCGGACTCGCCGACGACCACCGCGACCGGACCATCGTGCACACCGTGATTCAGCTTGCGCACGGGCTGGG
AATGGACGTGGTGGCCGAGGGCGTGGAAACGCCGACCAGTCTCGCGCTGTTGCGGCAAGCGGAGTGCGATACGGGGCAAGGCTTCCTGTTCGCCAAGCCA
GTGCCGGCGGCGGCATTCGCCGCCTTTGTCAGTCAATGGAGGGGTGCCACCATGAATGCAAATGATCCGACTGCCACCAGTTGCTGCGTGTGCTGCAAGG
AAATCCCGCTCGACGCCGCCTTCACACCGGAAGGTGCGGAGTACGTCGAGCGCTTCTGCGGGCTGGAGTGTTATCAACGCTTCCAGGCGCGGGCAAGCAA
CGCGACCGAAACGAGCGCCGAACTGAACGCTTGCGGTTCGCCGCCGTCAGATTGAGGCATACCCTATCCTGATGTCAGGATAGACCGCCTCACAACGTCA
GAATAGAGTCGATTGTGTTATTTATTGACACATGCAGAAAAAGGTCATAGATTTCTTCCTGACATTTTCGCCCAGGGAGGCATCTTGCAGGGTCAACGCA
TCGGCTACGTCCGGGTCAGCAGCTTCGACCAGAACCCGGAACGGCAACTTGAACACGTCGAAGTCGGCAAGGTGTTCACCGACAAGGCGTCGGGCAAGGA
CACCCAGCGGCCCGAGCTTGATTCGCTGCTGGCCTTCGTACGCGAAGGCGACACCGTGGTGGTTCATAGCATGGATCGCCTGGCGCGCAACCTCGATGAC
TTGCGCCGCCTCGTGCAAAAGCTCACCAAGCGCGGCGTGCGTATCGAGTTCGTCAAGGAAAGCCTGACCTTCACCGGCGAGGATTCGCCGATGGCGAACC
TAATGCTGTCGGTCATGGGGGCGTTCGCCGAATTCGAGCGGGCCTTGATCCGCGAGCGGCAGAGGGAAGGCATCGCGCTCGCCAAACAGCGCGGAGCCTA
CCGGGGCCGCAAGAAAGCGCTGTCGCCCGAACAGGTAGCCGATCTGCGGCAGCGGGCCGCCGCCGGCGAACAAAAAGCGAAGCTGGCCCGCGAGTTTGGT
GTCAGCCGGGAGACCCTGTATCAATACTTGAGAACGGATCAGTAAATATGCCACGTCGTTCAATCCTGTCCGCCGCCGAGCGGGAAAGCCTGCTGGCGTT
GCCGGACTCCAAGGACGACCTGATCCGACATTACACATTCAGCGATAGCGACCTCTCGATCATCCGACAGCGGCGCGGGCCTGCAAATCGTTTGGGCTTT
GCAGTTCAGCTCTGTTACCTGCGTTTCCCCGGCATCATTCTTGGTGTCGATCAGCCGCCGTTCCTACCTTTACTGAAACTGGTCGCCGACCAACTCAAGG
TCGGCATCGAAAACTGGGACGAGTACGGGCAGCGGGAGCAGACCCGGCGCGAGCACCTGGTCGAGCTGCAAGCCTTGTTCGGCTTTCAGCCGTTCACCAT
GAGCCACTACCGGCAGGCCGTGCACACGCTGACCGAGCTGGCCATGCAGACCGACAAAGGCATCGTGCTGGCCAGCGCCTTGATCGAACATCTGCGGCGG
CAGTCGGTCATTCTGCCTGCCCTCAACGCCGTCGAGCGGGCGAGCGCCGAGGCAATCACCCGTGCCAACCGGCGCATCTACGATGCCTTGGCCGAACCGC
TGTCGGACGCGCATCGCCGCCGCCTCGACGACCTGCTCAAGCGCCGCGATAACGGCAAGACGACCTGGCTGGCTTGGCTGCGCCAGTCGCCCGTCAAGCC
AAACTCGCGGCACATGCTGGAACACATCGAACGCCTCAAAGCGTGGCAGACGCTCGACCTGCCTTCGGGCATCGAGCGTCTGGTTCACCAGAACCGGCTG
CTCAAGATCGCCCGCGAAGGCGGTCAGATGACGCCCGCCGACTTGGCCAAGTTCGAGCCACAGCGCCGCTACGCAACCCTGGTGGCGCTTGCCATTGAAG
GCATGGCTACCGTCACCGACGAAATCATCGACCTACACGACCGCATCTTGGGCAAGCTGTTCAACGCCGCCAAGAATAAGCATCAGCAGCAGTTCCAGGC
GTCCGGCAAGGCCATCAACGCCAAGGTGCGCCTGTACGGACGCATCGGCCAGGCGCTGATCGACGCCAAGCAGTCGGGACGCGACCCGTTCGCCGCCATC
GAGGCCGTCATGTCCTGGGATGCTTTCGCCGAGAGCGTCACCGAGGCGCAGAAGCTCGCGCAGCCCGATGACTTCGATTTTCTGCATCGCATCGGCGAGA
GCTACGCCACTTTGCGCCGTTACGCGCCGGAATTTCTTGCTGTGCTCAAGCTGCGGGCGGCGCCCGCTGCCAAGAACGTGCTCGATGCCATCGAGGTGCT
GCGCGGCATGAACACCGACAACGCCCGCAAGGTGCCCGCCGATGCCCCGACCGATTTCATTAAGCCGCGCTGGCAGAAGCTGGTGATGACCGACGCCGGC
ATCGACCGGCGCTACTACGAGCTGTGCGCGCTATCGGAGCTGAAGAACTCACTGCGCTCGGGCGACATCTGGGTGCAGGGTTCGCGCCAGTTCAAGGATT
TCGAGGACTATCTGGTGCCGCCTGCGAAGTTCGCCAGTCTCAAGCAGTCCAGTGAATTGCCGCTGGCCGTGGCCACCGACTGCGATCAGTACCTGCACGA
GCGGCTGACGCTGCTGGAAACCCAGCTCGCCACCGTCAACCGCATGGCGGCGGCCAACAACCTGCCGGACGCCATCATCACCGAGTCGGGCCTGAAAATC
ACGCCGTTGGATGCGGCGGTGCCGGACACCGCGCAGGCGTTGATCGACCAAACGGCCATGATCCTGCCGCACGTCAAGATCACCGAGCTGCTGCTTGAAG
TCGATGAGTGGACGGGCTTCACCCGGCACTTCACACACCTGAAATCGGGCGATCTGGCCAAGGACAAAAATTTGCTGCTGACCACCATCTTGGCCGACGC
GATCAACCTGGGCCTGACCAAGATGGCCGAGTCCTGCCCCGGCACGACCTACGCCAAGTTGGCTTGGCTGCAAGCCTGGCATACCCGCGACGAAACCTAT
TCGACGGCATTGGCCGAATTGGTCAACGCCCAATTCCAGCACCCGTTCGCCGGGCACTGGGGCGACGGCACCACGTCATCGTCGGACGGCCAGAACTTCC
GAACCGGCAGCAAGGCCGAGAGTACCGGGCACATCAACCCAAAATATGGCAGCAGCCCAGGACGGACTTTCTACACCCATATCTCCGACCAGTACGCGCC
GTTCCATACCAAGGTGGTCAATGTCGGCGTGCGCGATTCGACCTATGTGCTCGACGGCCTGCTGTACCACGAGTCCGACTTGCGCATCGAGGAACACTAC
ACCGACACGGCGGGCTTCACCGATCACGTCTTCGCCTTGATGCACCTGCTGGGCTTCCGCTTCGCGCCGCGCATCCGCGACTTGGGCGACACCAAGCTGT
TCATCCCCAAGGGCGAAACCAATTACGATGCACTCAAGCCGATGATTAGCAGTGACAAGCTGAACATCAAGGCGATTCGTGCCCATTGGGACGAAATCTT
GCGGCTGGCCACCTCGATCAAGCAGGGCACAGTGACGGCCTCGCTGATGCTGCGCAAGCTCGGCAGCTACCCGCGCCAGAACGGTCTGGCCGTGGCGTTG
CGCGAGCTGGGCCGCATCGAACGCACGTTGTTCATCCTGGATTGGCTACAAAGCGTGGAACTGCGCCGCCGCGTGCATGCCGGGTTGAACAAGGGCGAAG
CCCGCAACGCGCTGGCCCGCGCCGTGTTCTTCAACCGCCTTGGCGAAATCCGCGACCGCAGTTTCGAGCAGCAGCGCTACCGAGCCAGCGGCCTCAACCT
GGTGACGGCGGCCGTCGTGTTGTGGAACACGGTCTATCTGGAACGGGCTGCACATGCGCTGCGCGGCAACGGCCATGCCGTCGATGACGCGCTGTTGCAA
TATCTGTCGCCGCTCGGCTGGGAGCACATCAATCTGACCGGCGATTACCTGTGGCGCAGCAGCGCCAAGATCGGCGCAGGCAAGTTCAGGCCGTTACGAC
CGCTGCAACCGGCTTAGCGTGCTTTATTTTCCGTTTTCTGAGGCGACCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
561 bp186 aa50855645+No
ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MQGQRIGYVRVSSFDQNPERQLEHVEVGKVFTDKASGKDTQRPELDSLLAFVREGDTVVVHSMDRLARNLDDLRRLVQKLTKRGVRIEFVKESLTFTGED
SPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSPEQVADLRQRAAAGEQKAKLAREFGVSRETLYQYLRTDQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
2970 bp989 aa56488617+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRRSILSAAERESLLALPDSKDDLIRHYTFSDSDLSIIRQRRGPANRLGFAVQLCYLRFPGIILGVDQPPFLPLLKLVADQLKVGIENWDEYGQREQTR
REHLVELQALFGFQPFTMSHYRQAVHTLTELAMQTDKGIVLASALIEHLRRQSVILPALNAVERASAEAITRANRRIYDALAEPLSDAHRRRLDDLLKRR
DNGKTTWLAWLRQSPVKPNSRHMLEHIERLKAWQTLDLPSGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALAIEGMATVTDEIIDLHDRI
LGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIGQALIDAKQSGRDPFAAIEAVMSWDAFAESVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVL
KLRAAPAAKNVLDAIEVLRGMNTDNARKVPADAPTDFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQ
SSELPLAVATDCDQYLHERLTLLETQLATVNRMAAANNLPDAIITESGLKITPLDAAVPDTAQALIDQTAMILPHVKITELLLEVDEWTGFTRHFTHLKS
GDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFQHPFAGHWGDGTTSSSDGQNFRTGSKAESTGHINPKY
GSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLFIPKGETNYDALKPMI
SSDKLNIKAIRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGE
IRDRSFEQQRYRASGLNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA

 

Blast result :
Comments
In the genome, this element is targetted by ISKpn10.
TnAs2 ORF9 (resolvase) is 95% aa similar to ISPa40,
and TnAs2 ORF10 (transposase) is 96% aa similar to ISPa38;

ORFs 1-8 are passenger genes:
ORF1 is distantly related to ISPpu12_aa1, a similar ORF is encoded on ISStma11 and ISThsp9,
ORF2: a similar ORF is encoded on ISStma11 and ISThsp9,
ORF3: a similar ORF is encoded on ISStma11 and ISThsp9,
ORF4: none of the ISFinder transposons codes for a similar ORF,
ORF5: a similar ORF is encoded on ISStma11; one to two similar ORFs are encoded on ISArsp6,
ORF6: tblastN matches in ISFinder may be casual,
ORF7: none of the ISFinder transposons codes for a similar ORF,
ORF8: none of the ISFinder transposons codes for a similar ORF.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20