ISBmu5

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010805 ND Burkholderia multivorans
Burkholderia multivorans ATCC 17616 chromosome 2
Burkholderia multivorans ATCC 17616 chromosome 1
DNA section
IS Length : 1447 bp

Ends


IR Length : 19/24

IRL : TGAACTGCACCCCAAAAGTTGGACTAGATCCAACCTTTGGGGTGTTTTTC
IRR : TGAACTGACCCCACGAAGTTGGACGGTTAAAATCTAAGCGGCCGAGGGCT

Insertion site


Left flankDirect repeatRight flankDR Length
AACCGGCCCGTGACGGGCCGGTT3
ACCGGCGGCCGAGGCTGCCGGTT3
TAGCGGCGCCCGCGGCGCCGTGC3
AACGGCGGCCGCGGGCCGCCGTT3
GGGCGGCGCCGCTGTCGCCGTTT3
AAGCGGCGCCCGGCGCGCCGCTT3

DNA sequence

TGAACTGCACCCCAAAAGTTGGACTAGATCCAACCTTTGGGGTGTTTTTCATGACGAAGTACGACGAACGTTTCCGACGTCGGGTGGTTCAGGCGTATCT
GGCTGGAGAGGCTGGGACCAAGACACTTGCGCATCGTTACGGCGTGGGTAGCACGATGGTCCGACGTTGGGTGGCGAGCTATCGCGAGCATGGCGAAGAC
GGACTGAGCAAGAAGCACAGCCACTACGACGCGCGATTCAAGCTATCGGTGCTCCAACGGATGTGGCGCGATGAGTTGTCATATGCCCGAGTGGCGGTGG
TATTCGGCATTCGCAATGAACGGAGCATCCCGGTTTGGGAGCGCCTGTATCATGAGGGCGGTATAGAAGCCCTGATGCCTCGTCGCCGAGGACGTCCCCC
GAAGATGACCACCTTGCCGCCACCGAAGCCTACCGATGATGCCGCGCAGAAAGAGCCGAGCCGCGAGGAACTGCTCAAGGAAATCGTGTATTTGCGCGCG
GAGGTGGCGTACCTAAAAAAGCTCGATGCGCTGCTGCAATCAAAGAAGCAGACAGCGCCCAGGAAAAAACGCAAATAATACGTGAGCTTAGGCAATGCCA
TCCGATCGCCGCGTTACTGAAGGCGGCAGGTCTGGCACGCAGCACGTTCTATTACCAACTCAAGGTATTGGATGCTGGCGATCGGTATGCGGAGCTCAAA
GTGAAGATCCGGAGCGTGTACGACCACCACAAGGGCCGTTACGGCTATCGGCGAATCACGACAGTGATCCGGCAAGCGGGACACGCGATCAATCACAAGA
CCGTGCAGCGGTTGATGGGACAACTGCAGTTGAAATCTCTGGTACGCCCGAAGAGATATCGTTCCTGGCGAGGCGAAGTCGGCCGCGTTGCCCCAAATCT
ACTGCGGCGGCAGTTTGATGCTGAACGCCCGAATCAGAAATGGGTGACCGACGTGACCGAATTCAATGTAGGGGGCCAAAAGCTCTATCTGTCGCCGGTG
ATGGACTTGTACAACGGGGAGATCGTCGCTTACCAAATGGATGAGCGCCCGAGCTTCGAGTTGGTCAGCGGCATGTTGAAGAAGGCGGTGGCCAAGCTCG
ATGATGAAGGACGGCCGTTACTGCATTCGGATCAAGGCTGGCAGTATCAGATGCCCGCCTACCGTCGGTTGTTGAAGCAGCATGCGCTCACCCAGAGCAT
GTCGCGCAGAGGCAACTGCCTTGATAACGCAGCCATGGAGAGCTTCTTCGGCACGCTGAAATCCGAGTGCTATCGGCTGGCGCGCTTTGCGAACGTGGAG
CAGTTGCGAGATGCGTTGAGCCGCTACATTCACTACTACAACCACGAACGCATCAAGCTCAAATTAAACGGGCTGAGTCCCGTGCAATACAGGACTCAGC
CCTCGGCCGCTTAGATTTTAACCGTCCAACTTCGTGGGGTCAGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
528 bp175 aa51578+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKYDERFRRRVVQAYLAGEAGTKTLAHRYGVGSTMVRRWVASYREHGEDGLSKKHSHYDARFKLSVLQRMWRDELSYARVAVVFGIRNERSIPVWERLY
HEGGIEALMPRRRGRPPKMTTLPPPKPTDDAAQKEPSREELLKEIVYLRAEVAYLKKLDALLQSKKQTAPRKKRK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
975 bp324 aa4401414+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

CRAERAEPRGTAQGNRVFARGGGVPKKARCAAAIKEADSAQEKTQIIRELRQCHPIAALLKAAGLARSTFYYQLKVLDAGDRYAELKVKIRSVYDHHKGR
YGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQM
DERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGTLKSECYRLARFANVEQLRDALSRYIHYY
NHERIKLKLNGLSPVQYRTQPSAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1364 bp454 aa511414+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKYDERFRRRVVQAYLAGEAGTKTLAHRYGVGSTMVRRWVASYREHGEDGLSKKHSHYDARFKLSVLQRMWRDELSYARVAVVFGIRNERSIPVWERLY
HEGGIEALMPRRRGRPPKMTTLPPPKPTDDAAQKEPSREELLKEIVYLRAEVAYLKKARCAAAIKEADSAQEKTQIIRELRQCHPIAALLKAAGLARSTF
YYQLKVLDAGDRYAELKVKIRSVYDHHKGRYGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVT
DVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFF
GTLKSECYRLARFANVEQLRDALSRYIHYYNHERIKLKLNGLSPVQYRTQPSAA

 

Blast result :
Comments
ISBmu5 is 94% aa similar to ISBcen10. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2015)
2] Zhang,W., Wu,Z. and Wang,R. (2012) Direct submission GenBank.