ISBmu5
- Family IS3
- Group IS150
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010805 | ND | Burkholderia multivorans | Burkholderia multivorans ATCC 17616 chromosome 2 Burkholderia multivorans ATCC 17616 chromosome 1 |
DNA section
IS Length : 1447 bp
Ends
IR Length : 19/24
IRL : TGAACTGCACCCCAAAAGTTGGACTAGATCCAACCTTTGGGGTGTTTTTC
IRR : TGAACTGACCCCACGAAGTTGGACGGTTAAAATCTAAGCGGCCGAGGGCT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
AACCGGCCCG | TGA | CGGGCCGGTT | 3 |
ACCGGCGGCC | GAG | GCTGCCGGTT | 3 |
TAGCGGCGCC | CGC | GGCGCCGTGC | 3 |
AACGGCGGCC | GCG | GGCCGCCGTT | 3 |
GGGCGGCGCC | GCT | GTCGCCGTTT | 3 |
AAGCGGCGCC | CGG | CGCGCCGCTT | 3 |
DNA sequence
TGAACTGCACCCCAAAAGTTGGACTAGATCCAACCTTTGGGGTGTTTTTCATGACGAAGTACGACGAACGTTTCCGACGTCGGGTGGTTCAGGCGTATCT
GGCTGGAGAGGCTGGGACCAAGACACTTGCGCATCGTTACGGCGTGGGTAGCACGATGGTCCGACGTTGGGTGGCGAGCTATCGCGAGCATGGCGAAGAC
GGACTGAGCAAGAAGCACAGCCACTACGACGCGCGATTCAAGCTATCGGTGCTCCAACGGATGTGGCGCGATGAGTTGTCATATGCCCGAGTGGCGGTGG
TATTCGGCATTCGCAATGAACGGAGCATCCCGGTTTGGGAGCGCCTGTATCATGAGGGCGGTATAGAAGCCCTGATGCCTCGTCGCCGAGGACGTCCCCC
GAAGATGACCACCTTGCCGCCACCGAAGCCTACCGATGATGCCGCGCAGAAAGAGCCGAGCCGCGAGGAACTGCTCAAGGAAATCGTGTATTTGCGCGCG
GAGGTGGCGTACCTAAAAAAGCTCGATGCGCTGCTGCAATCAAAGAAGCAGACAGCGCCCAGGAAAAAACGCAAATAATACGTGAGCTTAGGCAATGCCA
TCCGATCGCCGCGTTACTGAAGGCGGCAGGTCTGGCACGCAGCACGTTCTATTACCAACTCAAGGTATTGGATGCTGGCGATCGGTATGCGGAGCTCAAA
GTGAAGATCCGGAGCGTGTACGACCACCACAAGGGCCGTTACGGCTATCGGCGAATCACGACAGTGATCCGGCAAGCGGGACACGCGATCAATCACAAGA
CCGTGCAGCGGTTGATGGGACAACTGCAGTTGAAATCTCTGGTACGCCCGAAGAGATATCGTTCCTGGCGAGGCGAAGTCGGCCGCGTTGCCCCAAATCT
ACTGCGGCGGCAGTTTGATGCTGAACGCCCGAATCAGAAATGGGTGACCGACGTGACCGAATTCAATGTAGGGGGCCAAAAGCTCTATCTGTCGCCGGTG
ATGGACTTGTACAACGGGGAGATCGTCGCTTACCAAATGGATGAGCGCCCGAGCTTCGAGTTGGTCAGCGGCATGTTGAAGAAGGCGGTGGCCAAGCTCG
ATGATGAAGGACGGCCGTTACTGCATTCGGATCAAGGCTGGCAGTATCAGATGCCCGCCTACCGTCGGTTGTTGAAGCAGCATGCGCTCACCCAGAGCAT
GTCGCGCAGAGGCAACTGCCTTGATAACGCAGCCATGGAGAGCTTCTTCGGCACGCTGAAATCCGAGTGCTATCGGCTGGCGCGCTTTGCGAACGTGGAG
CAGTTGCGAGATGCGTTGAGCCGCTACATTCACTACTACAACCACGAACGCATCAAGCTCAAATTAAACGGGCTGAGTCCCGTGCAATACAGGACTCAGC
CCTCGGCCGCTTAGATTTTAACCGTCCAACTTCGTGGGGTCAGTTCA
GGCTGGAGAGGCTGGGACCAAGACACTTGCGCATCGTTACGGCGTGGGTAGCACGATGGTCCGACGTTGGGTGGCGAGCTATCGCGAGCATGGCGAAGAC
GGACTGAGCAAGAAGCACAGCCACTACGACGCGCGATTCAAGCTATCGGTGCTCCAACGGATGTGGCGCGATGAGTTGTCATATGCCCGAGTGGCGGTGG
TATTCGGCATTCGCAATGAACGGAGCATCCCGGTTTGGGAGCGCCTGTATCATGAGGGCGGTATAGAAGCCCTGATGCCTCGTCGCCGAGGACGTCCCCC
GAAGATGACCACCTTGCCGCCACCGAAGCCTACCGATGATGCCGCGCAGAAAGAGCCGAGCCGCGAGGAACTGCTCAAGGAAATCGTGTATTTGCGCGCG
GAGGTGGCGTACCTAAAAAAGCTCGATGCGCTGCTGCAATCAAAGAAGCAGACAGCGCCCAGGAAAAAACGCAAATAATACGTGAGCTTAGGCAATGCCA
TCCGATCGCCGCGTTACTGAAGGCGGCAGGTCTGGCACGCAGCACGTTCTATTACCAACTCAAGGTATTGGATGCTGGCGATCGGTATGCGGAGCTCAAA
GTGAAGATCCGGAGCGTGTACGACCACCACAAGGGCCGTTACGGCTATCGGCGAATCACGACAGTGATCCGGCAAGCGGGACACGCGATCAATCACAAGA
CCGTGCAGCGGTTGATGGGACAACTGCAGTTGAAATCTCTGGTACGCCCGAAGAGATATCGTTCCTGGCGAGGCGAAGTCGGCCGCGTTGCCCCAAATCT
ACTGCGGCGGCAGTTTGATGCTGAACGCCCGAATCAGAAATGGGTGACCGACGTGACCGAATTCAATGTAGGGGGCCAAAAGCTCTATCTGTCGCCGGTG
ATGGACTTGTACAACGGGGAGATCGTCGCTTACCAAATGGATGAGCGCCCGAGCTTCGAGTTGGTCAGCGGCATGTTGAAGAAGGCGGTGGCCAAGCTCG
ATGATGAAGGACGGCCGTTACTGCATTCGGATCAAGGCTGGCAGTATCAGATGCCCGCCTACCGTCGGTTGTTGAAGCAGCATGCGCTCACCCAGAGCAT
GTCGCGCAGAGGCAACTGCCTTGATAACGCAGCCATGGAGAGCTTCTTCGGCACGCTGAAATCCGAGTGCTATCGGCTGGCGCGCTTTGCGAACGTGGAG
CAGTTGCGAGATGCGTTGAGCCGCTACATTCACTACTACAACCACGAACGCATCAAGCTCAAATTAAACGGGCTGAGTCCCGTGCAATACAGGACTCAGC
CCTCGGCCGCTTAGATTTTAACCGTCCAACTTCGTGGGGTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
528 bp | 175 aa | 51 | 578 | + | No |
Description : First part of the transposase
ORF sequence :
MTKYDERFRRRVVQAYLAGEAGTKTLAHRYGVGSTMVRRWVASYREHGEDGLSKKHSHYDARFKLSVLQRMWRDELSYARVAVVFGIRNERSIPVWERLY
HEGGIEALMPRRRGRPPKMTTLPPPKPTDDAAQKEPSREELLKEIVYLRAEVAYLKKLDALLQSKKQTAPRKKRK
HEGGIEALMPRRRGRPPKMTTLPPPKPTDDAAQKEPSREELLKEIVYLRAEVAYLKKLDALLQSKKQTAPRKKRK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
975 bp | 324 aa | 440 | 1414 | + | No |
Description : Second part of the transposase
ORF sequence :
CRAERAEPRGTAQGNRVFARGGGVPKKARCAAAIKEADSAQEKTQIIRELRQCHPIAALLKAAGLARSTFYYQLKVLDAGDRYAELKVKIRSVYDHHKGR
YGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQM
DERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGTLKSECYRLARFANVEQLRDALSRYIHYY
NHERIKLKLNGLSPVQYRTQPSAA
YGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQM
DERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGTLKSECYRLARFANVEQLRDALSRYIHYY
NHERIKLKLNGLSPVQYRTQPSAA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1364 bp | 454 aa | 51 | 1414 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKYDERFRRRVVQAYLAGEAGTKTLAHRYGVGSTMVRRWVASYREHGEDGLSKKHSHYDARFKLSVLQRMWRDELSYARVAVVFGIRNERSIPVWERLY
HEGGIEALMPRRRGRPPKMTTLPPPKPTDDAAQKEPSREELLKEIVYLRAEVAYLKKARCAAAIKEADSAQEKTQIIRELRQCHPIAALLKAAGLARSTF
YYQLKVLDAGDRYAELKVKIRSVYDHHKGRYGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVT
DVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFF
GTLKSECYRLARFANVEQLRDALSRYIHYYNHERIKLKLNGLSPVQYRTQPSAA
HEGGIEALMPRRRGRPPKMTTLPPPKPTDDAAQKEPSREELLKEIVYLRAEVAYLKKARCAAAIKEADSAQEKTQIIRELRQCHPIAALLKAAGLARSTF
YYQLKVLDAGDRYAELKVKIRSVYDHHKGRYGYRRITTVIRQAGHAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVT
DVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSGMLKKAVAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFF
GTLKSECYRLARFANVEQLRDALSRYIHYYNHERIKLKLNGLSPVQYRTQPSAA
Blast result :
Comments
ISBmu5 is 94% aa similar to ISBcen10. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2015)
2] Zhang,W., Wu,Z. and Wang,R. (2012) Direct submission GenBank.
2] Zhang,W., Wu,Z. and Wang,R. (2012) Direct submission GenBank.