ISHvo23
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001956 | ND | Haloferax volcanii | Haloferax volcanii DS2 |
DNA section
IS Length : 1493 bp
Ends
Left end : CTGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATCTGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATCCGACACTTCCT II struct. : Yes
Right end : GCGGTTGCTTTGCACCGCGAGAACAGGTCGTGTCGTAGACTAGCAAATATCCCACCTGCGGTACGGGAAGCCCGGTCGTTCACGACGGGGAGGATGTCAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CGCTGGATAAGG | CTAT | CTGCGGCCTCGA | tcac |
DNA sequence
CTGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATCTGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATCCGACACTTCCT
ACGCAGTCCACCGTCGATGGCAGGCTGGATATTCCACGCCAATCCGTAGGATTAACTAAATCTTCCTACATAGGCTATGTATGGCGATTCAGGTCACTCG
CACCTACGTTGGTTCCATCCAGAACCACCAACAGGTCTGCGATGGCCTCGACTCGCTCGGAGATTCTGCCTCGAAAATCTGGAACGTCGCACGATGGACA
GCAGACCGCGTGTGGGACGAGGTTGGCAAAATCCCAAACGAAGGTTCGCTCAAATCGTACATGAAGAACCAAGCGTGCTGGAAAGACTTGAACGCACAAT
CCAGTCAGAAAGTCATCGAAGAACTTTCTGACGCTTTCCAGTCATGGTTCGACCTGCGACACAAAGACTCGAAGTCGAATCCGCCCGGCTACCGAAAACA
CGGCGACAAACGGCCACGTTCCACGGTCACGTTCAAAGCAGACGGATTCAAACACGACCCCGAGAACAATCGGGTTCGACTCAGCAAAGGCTCGAACCTC
AAAGAGCATTTCTCGGATTTCATCCTCTGTGAGTACCAGACTCGCCCGGACGTTGACCTCTCGGAAGTTGAATCGGTGCAGAACGTTCGCGCCGTCTGGA
ACGGCGACGAATGGGAACTGCACTTCGTCTGCAAAGTCAGTCTCGAAACCAACGACTCAGCAGGCGACAAGGTGGCAGGAATCGACCTCGGCATCAAGAA
CATCGCCACGGTCGCTTTCTCCGATGAATACGTTCTCTACCCCGGCAACTCGCTCAAACAGGACAAGCACTACTTCACGCGTGCTGAGTACGACACAGAA
GGTGAGAATGGCCCGTCCGAGAGGTTGATGTGGGCACGTCGGAAACTCGCAGAGCGCGAAACGCACTTCTACCATACACTGACGGACACTATCATCACAG
AGTGTGCCGAGCGCGGTGTTGGTACGCTTGCAGTGAGCTGGCCTGAAGACGTTCGTGACTCTGACTGGGGCAAGACTGGCAACAAGAAGTTGCATACGTG
GGCGTTCGACCGCATCTACCAGTACCTCGAATACAAAGGTAAGATTCGTGGCGTTGAGGTGCTGAAAGAGAACGAGTGGGACACCTCGAAGACGTGTTCA
CGGTGTGGAGACGACACGAAAGGGAACCGGAAACATCGTGGGTTGTACGTCTGCTCGTCGTGTGGGTTGGTCGGAAACGCGGATTGCAACGGGGCGGAGA
ATATGCGTCAGAAGATAACTCCGAGTCCTCACGGTGAGGATAGGAGTAACGGCTGTGTGGCACAGCCATCGGTACACCTGTTCGACTCAGAGAGCGGTTG
CTTTGCACCGCGAGAACAGGTCGTGTCGTAGACTAGCAAATATCCCACCTGCGGTACGGGAAGCCCGGTCGTTCACGACGGGGAGGATGTCAC
ACGCAGTCCACCGTCGATGGCAGGCTGGATATTCCACGCCAATCCGTAGGATTAACTAAATCTTCCTACATAGGCTATGTATGGCGATTCAGGTCACTCG
CACCTACGTTGGTTCCATCCAGAACCACCAACAGGTCTGCGATGGCCTCGACTCGCTCGGAGATTCTGCCTCGAAAATCTGGAACGTCGCACGATGGACA
GCAGACCGCGTGTGGGACGAGGTTGGCAAAATCCCAAACGAAGGTTCGCTCAAATCGTACATGAAGAACCAAGCGTGCTGGAAAGACTTGAACGCACAAT
CCAGTCAGAAAGTCATCGAAGAACTTTCTGACGCTTTCCAGTCATGGTTCGACCTGCGACACAAAGACTCGAAGTCGAATCCGCCCGGCTACCGAAAACA
CGGCGACAAACGGCCACGTTCCACGGTCACGTTCAAAGCAGACGGATTCAAACACGACCCCGAGAACAATCGGGTTCGACTCAGCAAAGGCTCGAACCTC
AAAGAGCATTTCTCGGATTTCATCCTCTGTGAGTACCAGACTCGCCCGGACGTTGACCTCTCGGAAGTTGAATCGGTGCAGAACGTTCGCGCCGTCTGGA
ACGGCGACGAATGGGAACTGCACTTCGTCTGCAAAGTCAGTCTCGAAACCAACGACTCAGCAGGCGACAAGGTGGCAGGAATCGACCTCGGCATCAAGAA
CATCGCCACGGTCGCTTTCTCCGATGAATACGTTCTCTACCCCGGCAACTCGCTCAAACAGGACAAGCACTACTTCACGCGTGCTGAGTACGACACAGAA
GGTGAGAATGGCCCGTCCGAGAGGTTGATGTGGGCACGTCGGAAACTCGCAGAGCGCGAAACGCACTTCTACCATACACTGACGGACACTATCATCACAG
AGTGTGCCGAGCGCGGTGTTGGTACGCTTGCAGTGAGCTGGCCTGAAGACGTTCGTGACTCTGACTGGGGCAAGACTGGCAACAAGAAGTTGCATACGTG
GGCGTTCGACCGCATCTACCAGTACCTCGAATACAAAGGTAAGATTCGTGGCGTTGAGGTGCTGAAAGAGAACGAGTGGGACACCTCGAAGACGTGTTCA
CGGTGTGGAGACGACACGAAAGGGAACCGGAAACATCGTGGGTTGTACGTCTGCTCGTCGTGTGGGTTGGTCGGAAACGCGGATTGCAACGGGGCGGAGA
ATATGCGTCAGAAGATAACTCCGAGTCCTCACGGTGAGGATAGGAGTAACGGCTGTGTGGCACAGCCATCGGTACACCTGTTCGACTCAGAGAGCGGTTG
CTTTGCACCGCGAGAACAGGTCGTGTCGTAGACTAGCAAATATCCCACCTGCGGTACGGGAAGCCCGGTCGTTCACGACGGGGAGGATGTCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 385 bp | 416 aa | 1815 | 1431 | + | No |
AG : TnpB
ORF sequence :
MAIQVTRTYVGSIQNHQQVCDGLDSLGDSASKIWNVARWTADRVWDEVGKIPNEGSLKSYMKNQACWKDLNAQSSQKVIEELSDAFQSWFDLRHKDSKSN
PPGYRKHGDKRPRSTVTFKADGFKHDPENNRVRLSKGSNLKEHFSDFILCEYQTRPDVDLSEVESVQNVRAVWNGDEWELHFVCKVSLETNDSAGDKVAG
IDLGIKNIATVAFSDEYVLYPGNSLKQDKHYFTRAEYDTEGENGPSERLMWARRKLAERETHFYHTLTDTIITECAERGVGTLAVSWPEDVRDSDWGKTG
NKKLHTWAFDRIYQYLEYKGKIRGVEVLKENEWDTSKTCSRCGDDTKGNRKHRGLYVCSSCGLVGNADCNGAENMRQKITPSPHGEDRSNGCVAQPSVHL
FDSESGCFAPREQVVS
PPGYRKHGDKRPRSTVTFKADGFKHDPENNRVRLSKGSNLKEHFSDFILCEYQTRPDVDLSEVESVQNVRAVWNGDEWELHFVCKVSLETNDSAGDKVAG
IDLGIKNIATVAFSDEYVLYPGNSLKQDKHYFTRAEYDTEGENGPSERLMWARRKLAERETHFYHTLTDTIITECAERGVGTLAVSWPEDVRDSDWGKTG
NKKLHTWAFDRIYQYLEYKGKIRGVEVLKENEWDTSKTCSRCGDDTKGNRKHRGLYVCSSCGLVGNADCNGAENMRQKITPSPHGEDRSNGCVAQPSVHL
FDSESGCFAPREQVVS
Blast result :
Comments
ISHvo23 is 73% aa (ORFB) similar to ISNph18.
This element is somewhat special at the 5-prime end. The sequence is:
CTATC TGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATC
TGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATC
CGACACT...
Thus, there is a 43 bp direct repeat right at the beginning of the element, just after the targetting sequence.
This element is somewhat special at the 5-prime end. The sequence is:
CTATC TGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATC
TGTCTTCGAGGAGAGAATCCCGCCGTTTACGGCGGGAGTGAATC
CGACACT...
Thus, there is a 43 bp direct repeat right at the beginning of the element, just after the targetting sequence.
References
1] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605