ISfinder Mirror Site

ISAs31

Family IS3
Group IS3

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP022426	ND	Aeromonas salmonicida	Aeromonas salmonicida Aeromonas veronii B565 Aeromonas salmonicida subsp. pectinolytica 34mel

DNA section

IS Length : 1322 bp

Ends

IR Length : 24/30

IRL : TGTCCCGTCCTGAATATGGTTTACACCTTTCCCCCACATAAACGGAGAGC
IRR : TGTCTAGTCCTGAAATAGGTTTACACATTTTCGGCTAGCATTTCTCCACT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATTTCAAGG	AGA	CCCAGCTGCA	3
TTTTTGTGGG	TAT	CAACCGGGTT	3
TTTTTGTTTT	TGA	TGAATAAAGA	3
CTTTTGTTAA	TAG	CGATGGATGG	3
TTTTTCCTTT	CTC	ACACAACAGC	3
ACCGGTAAAT	CAG	GTTCAATTTG	3
TTTTTATGCC	CAG	TTTATGAAGA	3
TTATTTGTGA	TTTT	CCCTGCCGTA	4
TTTTATTGCC	CC	GAATGGGATG	2

DNA sequence

TGTCCCGTCCTGAATATGGTTTACACCTTTCCCCCACATAAACGGAGAGCCCGATGGGACAAGGTGTGAAACGGACACAGCGCGATTACTCGCTGACTTT
TAAACTGGCACTGGTCGAGCAGATTGAAAAAGGCGAGCTCACCTACAAACAGGCTCAGGCGCGTTATGGCATTCAGGGCCGCTCCACCGTTCTGGTATGG
TTGCGTAAACATGGTCGGCAAGATTGGAGCCAGGGGGCTTCTGTGCGCGCCGGCAGGAGCATCACCATGCCTGACCCCGACAACCAGACACCCGAACAGC
GTATCAAGGAACTCGAGCAGCAGCTGGCGCTTATGAGCCAGAAGGCCCAGTTCTTTGAGGCCGTCGTCGATGTACTGAAGAACGACTACGGCGTCTCCAT
CGTAAAAAAGCGACCCGGCAAGTCCTCTCGCAGCGGCAAGTCGAGAGGCTGACCATTGTCAGGGCTTGCCTGTTTCTGGGAATAAGCCGTCAGGCTTACT
ACAAGCGCAATCGGGTTGCAGACGAGCGCCATGCACAGGGGCTGCAGGTGGTGCGCTTCGTGCGTCAGGTACGGCTGCGACAACCGCGGGTCGGCACCCG
CAAGCTGCATTATCTGCTCCAGGGTCAGGATGATGTGTGGCTCAAGGTCGGGCGGGACAGGCTGTTTCGGATATTGGCCGAGCATCGCCTGCTGGTGCAA
CCCCGGCGGGCGTATCACAAGACCACCCACAGTTTTCACCGTTTCTACCGTCATCCCAACTTGCTCAAGGCGGGGCCTGAGCAGGTCACGCCGGTGGCTC
CGGAGCAAGTCTGGGTCGCCGACATCACCTATCTGCCAGCCAGGAGCGGGCCGCTGTACCTGAGCCTGGTGACAGATGCGTACTCACGCAAGATAGTGGG
CCATCACGTGCACGAAGGGATGCACGCAGAGTCGGTGGCGATGGCGTTCAAGAAAGCACTGAAGCAACGGCGTGGCGGCGGGGAGCTTATCCACCACTCG
GACCGCGGCGTGCAGTATTGCTCGGGCCTGTATCAGTCATTGCATGCACGGTACGGGGTGAAATGCTCGATGACGGATGGCTATGACTGTTACCAGAATG
CGTTGGCAGAGCGGGTGAACGGAATTTTGAAAGGAGAGTTGTTGTTGCAAAGCCCACAGGACCTGGCGCAAGCGCGGGAGATGGTGCGTGAGGCGGTGGA
TATCTACAACGTGGAGCGGCCGCATCATGCGTTGAAATACAGAACCCCCGATGCAGTACATCGGGGGTTCTGAGTGGAGAAATGCTAGCCGAAAATGTGT
AAACCTATTTCAGGACTAGACA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
399 bp	132 aa	54	452	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGQGVKRTQRDYSLTFKLALVEQIEKGELTYKQAQARYGIQGRSTVLVWLRKHGRQDWSQGASVRAGRSITMPDPDNQTPEQRIKELEQQLALMSQKAQF
FEAVVDVLKNDYGVSIVKKRPGKSSRSGKSRG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
915 bp	304 aa	359	1273	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GRRRCTEERLRRLHRKKATRQVLSQRQVERLTIVRACLFLGISRQAYYKRNRVADERHAQGLQVVRFVRQVRLRQPRVGTRKLHYLLQGQDDVWLKVGRD
RLFRILAEHRLLVQPRRAYHKTTHSFHRFYRHPNLLKAGPEQVTPVAPEQVWVADITYLPARSGPLYLSLVTDAYSRKIVGHHVHEGMHAESVAMAFKKA
LKQRRGGGELIHHSDRGVQYCSGLYQSLHARYGVKCSMTDGYDCYQNALAERVNGILKGELLLQSPQDLAQAREMVREAVDIYNVERPHHALKYRTPDAV
HRGF

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1220 bp	406 aa	54	1273	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGQGVKRTQRDYSLTFKLALVEQIEKGELTYKQAQARYGIQGRSTVLVWLRKHGRQDWSQGASVRAGRSITMPDPDNQTPEQRIKELEQQLALMSQKAQF
FEAVVDVLKNDYGVSIVKKATRQVLSQRQVERLTIVRACLFLGISRQAYYKRNRVADERHAQGLQVVRFVRQVRLRQPRVGTRKLHYLLQGQDDVWLKVG
RDRLFRILAEHRLLVQPRRAYHKTTHSFHRFYRHPNLLKAGPEQVTPVAPEQVWVADITYLPARSGPLYLSLVTDAYSRKIVGHHVHEGMHAESVAMAFK
KALKQRRGGGELIHHSDRGVQYCSGLYQSLHARYGVKCSMTDGYDCYQNALAERVNGILKGELLLQSPQDLAQAREMVREAVDIYNVERPHHALKYRTPD
AVHRGF

Blast result :

Comments

ISAs31 is 78% aa similar to ISSen1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20
3] Li Y., Liu Y., Zhou Z., Huang H., Ren Y., Zhang Y., Li G., Zhou Z. and Wang,L. (2011) J. Bacteriol. 193 (13), 3389-3390