ISAs31

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas veronii B565
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1322 bp

Ends


IR Length : 24/30

IRL : TGTCCCGTCCTGAATATGGTTTACACCTTTCCCCCACATAAACGGAGAGC
IRR : TGTCTAGTCCTGAAATAGGTTTACACATTTTCGGCTAGCATTTCTCCACT

Insertion site


Left flankDirect repeatRight flankDR Length
AATTTCAAGGAGACCCAGCTGCA3
TTTTTGTGGGTATCAACCGGGTT3
TTTTTGTTTTTGATGAATAAAGA3
CTTTTGTTAATAGCGATGGATGG3
TTTTTCCTTTCTCACACAACAGC3
ACCGGTAAATCAGGTTCAATTTG3
TTTTTATGCCCAGTTTATGAAGA3
TTATTTGTGATTTTCCCTGCCGTA4
TTTTATTGCCCCGAATGGGATG2

DNA sequence

TGTCCCGTCCTGAATATGGTTTACACCTTTCCCCCACATAAACGGAGAGCCCGATGGGACAAGGTGTGAAACGGACACAGCGCGATTACTCGCTGACTTT
TAAACTGGCACTGGTCGAGCAGATTGAAAAAGGCGAGCTCACCTACAAACAGGCTCAGGCGCGTTATGGCATTCAGGGCCGCTCCACCGTTCTGGTATGG
TTGCGTAAACATGGTCGGCAAGATTGGAGCCAGGGGGCTTCTGTGCGCGCCGGCAGGAGCATCACCATGCCTGACCCCGACAACCAGACACCCGAACAGC
GTATCAAGGAACTCGAGCAGCAGCTGGCGCTTATGAGCCAGAAGGCCCAGTTCTTTGAGGCCGTCGTCGATGTACTGAAGAACGACTACGGCGTCTCCAT
CGTAAAAAAGCGACCCGGCAAGTCCTCTCGCAGCGGCAAGTCGAGAGGCTGACCATTGTCAGGGCTTGCCTGTTTCTGGGAATAAGCCGTCAGGCTTACT
ACAAGCGCAATCGGGTTGCAGACGAGCGCCATGCACAGGGGCTGCAGGTGGTGCGCTTCGTGCGTCAGGTACGGCTGCGACAACCGCGGGTCGGCACCCG
CAAGCTGCATTATCTGCTCCAGGGTCAGGATGATGTGTGGCTCAAGGTCGGGCGGGACAGGCTGTTTCGGATATTGGCCGAGCATCGCCTGCTGGTGCAA
CCCCGGCGGGCGTATCACAAGACCACCCACAGTTTTCACCGTTTCTACCGTCATCCCAACTTGCTCAAGGCGGGGCCTGAGCAGGTCACGCCGGTGGCTC
CGGAGCAAGTCTGGGTCGCCGACATCACCTATCTGCCAGCCAGGAGCGGGCCGCTGTACCTGAGCCTGGTGACAGATGCGTACTCACGCAAGATAGTGGG
CCATCACGTGCACGAAGGGATGCACGCAGAGTCGGTGGCGATGGCGTTCAAGAAAGCACTGAAGCAACGGCGTGGCGGCGGGGAGCTTATCCACCACTCG
GACCGCGGCGTGCAGTATTGCTCGGGCCTGTATCAGTCATTGCATGCACGGTACGGGGTGAAATGCTCGATGACGGATGGCTATGACTGTTACCAGAATG
CGTTGGCAGAGCGGGTGAACGGAATTTTGAAAGGAGAGTTGTTGTTGCAAAGCCCACAGGACCTGGCGCAAGCGCGGGAGATGGTGCGTGAGGCGGTGGA
TATCTACAACGTGGAGCGGCCGCATCATGCGTTGAAATACAGAACCCCCGATGCAGTACATCGGGGGTTCTGAGTGGAGAAATGCTAGCCGAAAATGTGT
AAACCTATTTCAGGACTAGACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa54452+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGQGVKRTQRDYSLTFKLALVEQIEKGELTYKQAQARYGIQGRSTVLVWLRKHGRQDWSQGASVRAGRSITMPDPDNQTPEQRIKELEQQLALMSQKAQF
FEAVVDVLKNDYGVSIVKKRPGKSSRSGKSRG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
915 bp304 aa3591273+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GRRRCTEERLRRLHRKKATRQVLSQRQVERLTIVRACLFLGISRQAYYKRNRVADERHAQGLQVVRFVRQVRLRQPRVGTRKLHYLLQGQDDVWLKVGRD
RLFRILAEHRLLVQPRRAYHKTTHSFHRFYRHPNLLKAGPEQVTPVAPEQVWVADITYLPARSGPLYLSLVTDAYSRKIVGHHVHEGMHAESVAMAFKKA
LKQRRGGGELIHHSDRGVQYCSGLYQSLHARYGVKCSMTDGYDCYQNALAERVNGILKGELLLQSPQDLAQAREMVREAVDIYNVERPHHALKYRTPDAV
HRGF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1220 bp406 aa541273+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGQGVKRTQRDYSLTFKLALVEQIEKGELTYKQAQARYGIQGRSTVLVWLRKHGRQDWSQGASVRAGRSITMPDPDNQTPEQRIKELEQQLALMSQKAQF
FEAVVDVLKNDYGVSIVKKATRQVLSQRQVERLTIVRACLFLGISRQAYYKRNRVADERHAQGLQVVRFVRQVRLRQPRVGTRKLHYLLQGQDDVWLKVG
RDRLFRILAEHRLLVQPRRAYHKTTHSFHRFYRHPNLLKAGPEQVTPVAPEQVWVADITYLPARSGPLYLSLVTDAYSRKIVGHHVHEGMHAESVAMAFK
KALKQRRGGGELIHHSDRGVQYCSGLYQSLHARYGVKCSMTDGYDCYQNALAERVNGILKGELLLQSPQDLAQAREMVREAVDIYNVERPHHALKYRTPD
AVHRGF

 

Blast result :
Comments
ISAs31 is 78% aa similar to ISSen1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20
3] Li Y., Liu Y., Zhou Z., Huang H., Ren Y., Zhang Y., Li G., Zhou Z. and Wang,L. (2011) J. Bacteriol. 193 (13), 3389-3390