ISAs29

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas media WS
Aeromonas salmonicida subsp. salmonicida A449
Aeromonas salmonicida subsp. salmonicida A449 plasmid 5
Aeromonas salmonicida subsp. salmonicida A449 plasmid 4
Aeromonas salmonicida
Aeromonas salmonicida subsp. salmonicida culture-collection HER:1104 plasmid pAs
Klebsiella pneumoniae subsp. pneumoniae KPNIH27 plasmid pKEC-dc3
Pantoea sp. PSNIH2 plasmid pPSP-100
Enterobacter cloacae ECNIH2 plasmid pKEC-39c
Klebsiella oxytoca KONIH1 plasmid pKOX-86d
Citrobacter freundii CFNIH1 plasmid pKEC-a3c
Shewanella sp. ANA-3 chromosome 1
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 2613 bp

Ends


IR Length : 11/12

IRL : TGCATATTCCGGCCGATCATGAACACCGATTCCGGTTCAACGTGAACACC
IRR : TGCGTATTCCGGTGAAGTTGAACAGCCATTCCGGCCAACGTGAACACCCT

Insertion site


Left flankDirect repeatRight flankDR Length
TTCGCACCTTCCCTAGTGAACAACGGTGA5
ATCGGTCAGTTTGTTGTCCCCGGGCACAG5
AGAGTGGGCGAGCTGGCCACACCGCTCAT5
AACCCGTATCACACGTTAAGGCAGACTAT5

DNA sequence

TGCATATTCCGGCCGATCATGAACACCGATTCCGGTTCAACGTGAACACCATTTCCGGACCAATGTGAACGCTCATTCCGGCTCAACCTGAACACTTTGC
CCCTCCTTTCCGGAAACAGTGTTCAACATCCCGGAATCACTGTTCACATTCACCAGAATCATCCTTAACGCATTGATTTAACAACCTTTGATACGCTCTC
CCTTTTGCCAGGGAAGAGCCTATGTCTGCCAAGAGGATTGCAATGCGTAAAATTCGTGAAGTGCTGCGACTGCGGCTGGAAGCTGACCTCTCTGTTCGCC
AGATAAGTTGCTGCACCAAGGTCAGCGTTGGCTCCATCCAGAAACTCCTCAAAAGTGCCCAGGCGCTCGGCCTGACCTGGCCCTTGCCCACCGAGCTTGA
TGATGGCCGACTGGCTGCCCTGTTCTATCCGCAGGCCGATACCACCATCTCGGCCCGCCATGTCGTGCCGGATTGGCCCTCTGTCCATCAGGAGCTCAAG
CGCAAGGGGGTCTCCAAACAGCTGCTCTGGGAGGAGTACACCCAGCGCTATCCCAATAGCTGTTACAGCTACTCCCAGTTCTGCGACCGTTACAAGTCCT
GGTGTCAGTTGCAAAAGCGCTCCATGCGCCAGATCCATAAGGCGGGGGAAAAGCTCTTTATCGACTACTGCGGGCCCACGGTGCCCATCGTGTCGCCGAC
CACGGGTGAGGTCCGTCAGGCACAAGTCTTTGTCGCCGTGCTCGGTGCGTCCAACTACACCTTTGCCGAGGCCACCTGGTCACAGTCACTGCCGGACTGG
CTGCAAAGCCATGTCCGTGCCTTTGAGTTTTTCGGCGGGACACCGGCACTGCTGATCCCCGACAACCTCAAGAGCGGTGTCAGCAAGGCTTGCCGATACG
ATCCCGAGCTCAACCCCAGCTACCAGCAGTTGGCCGAGCACTATCAGGTGGCGGTGATGCCAGCTCGTCCTTACAAGCCCAAGGACAAGGCCAAGGCGGA
GGTGGGTGTGCAGATCGTCGAGCGCTGGATCCTGGCCCGTCTACGCCATCAGACCTTCTTCTCGCTTGCCGAACTCAACCAGTGCATCCGTGCCTTGCTG
AACGAACTCAACGAGCGTCCGTTCAAGCAACTGCCCGGTAACCGACGTGACGCATTTGAGCAGTTGGACCGCCCGGCACTGGGACCACTGCCGGTGCATC
CCTATCGCTATGTCGCCATCAAAACGGTCAAGGTCAACATCGACTATCACGTCAGCTATGAGCAGCACCATTACTCGGTGCCACACCAGTATGTGGGTCA
GCAACTGGAGCTCCATGCAGGCGACACACTGCTTCAGGTCTATCACCAGCAACAGCTGGTCGCGAGCCATCCACGTAAAACCATCCCGGGGATGAGCACG
CTCCCTGAGCACATGCCGGAACGTCACAGCAAGCAGCAACGCTGGACCCCAGGGCGTCTTAAACAGTGGGCTGCTGACATCGGACCGAGCACCTTGTGCT
GGGTTAGCGAACGGTTGGAAGAGAAAGCCCATGCCGAGCAGGCATACCGACTCTGCCTGGGGCTGCTGAGTCTTAGCCGGGAGTATCCGCCATCACGGGT
TGAAGCCTGTTGCCAGTTGGCCAACCGGGAAGGTCTGGTGCGACTCAAGCAGCTCAAGTCGGTGCTGACCAGTGGCCGCGACCAACTGCCCGCAAGCCCG
CTGAGCTACCCAGAGTTACCGCAAGAGCACGAGAACATCCGTGGCCCCCAGAGCTTCCACTAAAGAGGATACGCCCCATGACCCAACAAACCCTGACCCG
CCTGAGAAACCTGAAACTGACCGGCATGGCGGATGCCGTGCAACAACAACTGGAACATGCGAGTACCTACGAAGGGCTGCCCTTCATCGAGCGGCTAAGC
CTGCTGGTCGAACACGAACAACTGAGCCGGGAGCAGCGTAAGCAAACTCGGCTGGTCAAGCAGGCAAGGCTCAAACTCCAGGCCACGGTGCAGGAGCTGG
ACTACCAGTCAGCACGCAACCTGGAGCGGTCACAGGTAGCAAACCTGGCCCAAGGAGAGTGGCTGCGCCGGGGCCAGAACCTGCTCATTACCGGCCCCTG
TGGCTGTGGAAAAACCTACCTGGCTTGTGCGCTGGGCTACCAAGCCTGTCAGCAAGGGCATAGCACTCGTTACTACCGGCTGCCACGTCTGCTGCTTGAG
CTGAGCCAGGCCAAGGTAGATGGCAGTTACGCCAGACTGTTGGGTCAACTGGCAAGGATAGAGCTGCTGATCCTGGATGACTGGGGACTGGAGGCGGTGA
ATGCCGAACAGCGCAACGCACTGCTGGAGATCATGGATGATCGGTATGGGAAATACTCGACGATAGTGGTCAGTCAGCTGCCAACTGAAGAGTGGTACGC
GAGCTTGGGTGACAACACCCTGGCAGATGCGATCCTGGACCGGCTGATGCACAACGCTCACCGGCTGTCACTGAAGGGAGAATCGATGAGGAAGAGGAGA
AGTGAGTTGACCCAATCTGAACAGTCGAGTTAGAAACGAGATGACGTGAGCGCGTTAAGGATCAGGGTGTTCACGTTGGCCGGAATGGCTGTTCAACTTC
ACCGGAATACGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1542 bp513 aa2221763+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSAKRIAMRKIREVLRLRLEADLSVRQISCCTKVSVGSIQKLLKSAQALGLTWPLPTELDDGRLAALFYPQADTTISARHVVPDWPSVHQELKRKGVSKQ
LLWEEYTQRYPNSCYSYSQFCDRYKSWCQLQKRSMRQIHKAGEKLFIDYCGPTVPIVSPTTGEVRQAQVFVAVLGASNYTFAEATWSQSLPDWLQSHVRA
FEFFGGTPALLIPDNLKSGVSKACRYDPELNPSYQQLAEHYQVAVMPARPYKPKDKAKAEVGVQIVERWILARLRHQTFFSLAELNQCIRALLNELNERP
FKQLPGNRRDAFEQLDRPALGPLPVHPYRYVAIKTVKVNIDYHVSYEQHHYSVPHQYVGQQLELHAGDTLLQVYHQQQLVASHPRKTIPGMSTLPEHMPE
RHSKQQRWTPGRLKQWAADIGPSTLCWVSERLEEKAHAEQAYRLCLGLLSLSREYPPSRVEACCQLANREGLVRLKQLKSVLTSGRDQLPASPLSYPELP
QEHENIRGPQSFH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17782533+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTQQTLTRLRNLKLTGMADAVQQQLEHASTYEGLPFIERLSLLVEHEQLSREQRKQTRLVKQARLKLQATVQELDYQSARNLERSQVANLAQGEWLRRGQ
NLLITGPCGCGKTYLACALGYQACQQGHSTRYYRLPRLLLELSQAKVDGSYARLLGQLARIELLILDDWGLEAVNAEQRNALLEIMDDRYGKYSTIVVSQ
LPTEEWYASLGDNTLADAILDRLMHNAHRLSLKGESMRKRRSELTQSEQSS

 

Blast result :
Comments
ISAs29 is 69% (orfA, the transposase) and 69% (orfB) aa simiar to ISAfe12.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20