ISAs30

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1426 bp

Ends


IR Length : 16/18

IRL : TAATGCCGATCAGTTAAGGATCCATTGACCGATCCTGCTGATGTGTAAAA
IRR : TAATGCCAGTCAGTTAAGCCTGACTGGCATTGTTTTTATTGGCTTTTTTT

Insertion site


Left flankDirect repeatRight flankDR Length
AATAAACAGGCCCTTCATGGGCCTGTTTTGAT12
GTAAAAAAGCCCCTCGCGGGGCTTTTTTCATT12
CAAAAACAGGGCCTGATGGCCCTGTTTTTGTT12
ACGAAAAGACCGCCCGATGGGCGGTCTTTTGC12
TAAAAAGGAGCGCAAACTGGCGCTCCTTTTTA12
GCGAAAGGCGGACCCTATGCCCTCTCCATCAA12
TAAAAAGAGCGACCCAAGGGTCGCTCTTTTTC12
TAAAAAAGGGCGGCGTGAAGCCGCCCTTTTCT12
AGTAAAAGGACCCGAAGGTCCTTTGCTGTTTG12
TGAAAAAGAGGGCCTTTTGGCCCTCTTTTCTA12
TAATTCAGAGGGGTCGGGGCCTGCCATTTGGC12
TAAAAAGGGCTCCTGTCGGGAGCCCTTTTTC11
GAGACAGGGGCCCCCGATGGACGGCTATTTT11
TGAAAAAGGGACCTTTCGGTCCCTTTTTCTA11
TCAAAAAAGGCGCCAAAGGGCGCCGTTTTTC11
ACAAAAAAGGCGAACATTATGTTCGCCTTTTTT13

DNA sequence

TAATGCCGATCAGTTAAGGATCCATTGACCGATCCTGCTGATGTGTAAAAATCCGAAACCTGTTGATTTGGTTTCGGATTTTTTATGCGTATCGAACAGG
CTCTCGATTTCCTTCACGCCAGCAATGCTGCTCAGTTTGAGTCACTCTCCGATCTCATTCCCAACGAGCTCATCACTACCCTGCTCGCCGAAGAGGGCGT
CGCTACCCTGCGCCGTCGCCGCATGCCCATGGAGCGTCTGGTCTGGGCCATCATCGGCATGGCCATGTTCCGCCACGTCCCCATGACCCAGCTGGTCAAT
CAGCTCGATATCCTCTTGCCCGGTGACCGCCCCTTTGTCGCCCCCAGCGCCTTCCTGCAAGCTCGCCAAAAGCTTGGCCACAAGAGCATTGAGCGGCTGT
TTCACGAAACCGCCACAGGCTGGCACCAGCAGGCCAATCACCCCGGCTGGGCCGGGCTGCAACTGCTCGCCGTTGATGGCGTGATGTGGCGCACGCCTGA
TACCCCGGACAATGCCGCCGCCTTTGCCAAGCCCGGTACCCAGCATGGGGAGACCGCTTATCCGCAGGTGCGCATGCTCTGCCAGATGGAGCTGACCAGT
CATCTGCTTGTTCAGGCTGTCATGGAGAGCTGCGCCGTCAACGAAATGGCGCTGGCCGAACAGCTCATTGCACGCACACCGGACCACTCGCTCACCCTGT
TCGACAAGGGGTTTTACTCCCTCGGTCTGCTACACGCCTGGCAAACTGCTGGCAGCGAGCGTCACTGGCTGCTGCCGCTCAAAAAGGGCACGCAGTACGA
GGTGGTACATAAGCTGGGGCGCCAGGATGTACTGGTGCAGCTCAAGACCAGTCCACAGGCACGCAAGAAGTGGCCGCAACTGCCGGACACAGTGGAAGCC
CGCTTGCTGACCCGCACTATCAACGGCAAGGAGCGGCAGGTACTGACCTCAATGGTCGACCCGATGCGCTTTCCGGGTGCCGAGATTGTCGAGCTCTACA
GCCATCGCTGGGAAATCGAGTTGGGCTATCGGGAGATGAAGCACAGCCTGCAACAACACCGACTGACCCTGCGTAGCAAGAAGGCAATGGGGATACGGCA
AGAGCTGTGGGGCGTGTTGTTGGCGTACAACCTGCTGCGCAGCCAGATGGTGAAGATGGCGGCGAGTCTAAAGGGATACACAGCAAGCCAGCTGAGCTTT
CACATGGCATCGGTGTACCTCATCCATGAACTGAGTTGCATGCCGTACATGTCGCCGGGGACTATCCCGAAGCGGGTGGCGGAGCTGGAGCAGCAGGCGG
GGCAGTTTGTGCTGCCGGCACGAAGGGAACGAAGCTACCCCCGCTGCGTGAAGCCAAGGCCACAGAAATACAGTGTAAAAAAAGCCAATAAAAACAATGC
CAGTCAGGCTTAACTGACTGGCATTA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1329 bp442 aa851413+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRIEQALDFLHASNAAQFESLSDLIPNELITTLLAEEGVATLRRRRMPMERLVWAIIGMAMFRHVPMTQLVNQLDILLPGDRPFVAPSAFLQARQKLGHK
SIERLFHETATGWHQQANHPGWAGLQLLAVDGVMWRTPDTPDNAAAFAKPGTQHGETAYPQVRMLCQMELTSHLLVQAVMESCAVNEMALAEQLIARTPD
HSLTLFDKGFYSLGLLHAWQTAGSERHWLLPLKKGTQYEVVHKLGRQDVLVQLKTSPQARKKWPQLPDTVEARLLTRTINGKERQVLTSMVDPMRFPGAE
IVELYSHRWEIELGYREMKHSLQQHRLTLRSKKAMGIRQELWGVLLAYNLLRSQMVKMAASLKGYTASQLSFHMASVYLIHELSCMPYMSPGTIPKRVAE
LEQQAGQFVLPARRERSYPRCVKPRPQKYSVKKANKNNASQA

 

Blast result :
Comments
ISAs30 is 71% aa similar to ISSba6.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20