ISAs28

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas media WS
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 2626 bp

Ends


IR Length : 37/49

IRL : TGCATATTCCCACGCATGGCGATCGCTCAATAACATTCATGCCGATCACT
IRR : TGGCTAATAACATCATGTCGATCAGCCAATAACATCATGCCGATCACCGT

Insertion site


Left flankDirect repeatRight flankDR Length
ATATCACCTACTTGCCGTCACTCGT5
ACAGGATACTGATGGGCTTGAGGTGA6

DNA sequence

TGCATATTCCCACGCATGGCGATCGCTCAATAACATTCATGCCGATCACTCAATAACATCCATGCCGATCACTCAATAACATCCCAAGCCGATCACTTTT
TACCCTCGATGGGAAACCATGATCGGCATCCATGTTATTGGTGACTTTTTCTCCCCGCTTCGTTAAACCCGCACTTTCCCATCTGCTCCAACAGAGGAAC
GTGCATGTCTGCGAGGCCTATCACTGTGAGAAAACTCAAAGAAATTCTTCGTCTGAAATATCAGGCTCAATTGGCTCATCGGCAGATCGCCCTCAGCCTG
CATATCTCACCCAGCACCGTATCCTATTACCTCAACCGCGCTGCTCAACTGGGCATCCTGACCTGGCCCCTGTCTGAGTCCTGGGATGATGTCTCCCTGG
CGCGCGCTTTCCTCAACACACGCGCCGTCCATAACACTCAGCACAAACCCACGCCCAACTGGGCCTTGCTGCATCAGGAGCTCAAAAAAACAGGTCAAAA
AGGCGTGACCCTCGAGCTGCTATGGCAGGAGTACGCCGAGCGCCATTCTGGCTCCCACTACAGCTACAACCACTTCTGTCGGCTCTATAAAGAGTGGGCG
GCCCTGCTGCAACCCTCGATGCGGCAGACCCATATTGCGGGCGAAAAACTCTTTGTCGACTACTGCGGCCAGACCATGCCGATTGTCGATCCGCACACCG
GCGAAATCCTTTACCGGGCCCAGATCTTTGTCGCCGTGCTGGGTGCTTCCAACTACACCTTTGCCGAGGCCACCCGCTCCCAGCAGCTCGAGGACTGGGT
CATGAGCCACAAGCGCGCTTTCGAGTTCTTTGGCGGTGTGCCGCAATTGGTGGTACCCGATTGCCTGAAGAGTGCCGTCAGCGTGGCGCGCAATACCGAT
CCGGATCTCAACCCCACCTACCAGATGCTGGCGGCCCATTTCGGTACGGCGATCATGCCCGCGCGGCCCCGCAAGCCGAAGGATAAGTCCAAGGCAGAGG
TGGGCGTGCAGATCGTCACCCGCTGGATCCTGGCGGTGCTGCGTCATGAAACCTTCCATAGCCTGGCCCAGCTCAATCAGCGGATGGGTGAACTGCTGAC
CCGGCTCAACCAGAAACCCTTCCAGAAGCTTCCTGGCTGCCGTGCCTCACAGTTTGCCGAGTTCGATGCTCCGGCGCTCAAGCCGCTGCCGGATTATCCC
TATCACTACACCAAAGTGATGTTGGTACTGGTTGGTAAGGACTACCACGTCGATCTGGAAAAGCACTATTACTCCGTGCCCCATGCCTTGCAGGGCAAGC
GGCTGGAGGCTCATCTGGCCGAACATACCGTCACGCTGTACCACGCCGGCAAGGTCGTGGCCGAGCACCCGCGGAGTCATCGCAAAGGTCAGCACAGCAG
CCTGCCTGCCCATATGCCCGCGCATCACCAGGCGCTGCTGGAATGGTCGCCCGAGCGTCTGCTCGGCTGGGCGGTATCCATCGGACCCTACACCACGCAT
TGGGTGGATGGTTTTATCCGGAATGTGGCACACCCGGCGCAAGCGGTGCGACCTTGTCTGGCCTTGCTGGGGCAGAGCAAAACCTATGGCAAGGAGCGGC
TGGAAGCGGCCTGCCTCCGGGGTTACCTGACCGGCGCCAACCGGCTGCACAACATCCGCAGCATGCTCAAGAACGGCCTCGAGCAACAACCCATCGCCAG
CAAGCGACAGGATCCGCTGCAAGACATCCGTCATGACAATGTCCATGGCGAGCACTACTTCCACTAACCCCCACAGCTCAGGAGAAGCCCATGCAATTAC
ACCTCAATGAACAACTAAGCCGCCTGCACTTGAGCGGTATGAAACAGGCCCTGAACCAGCAGCAGGCCCAGAGCATGCTGTACCTGGATATGGGTTTTGA
AGAGCGGCTGCAACTGTTACTGAGCCATGAGCTGGTGCAACGGGAGCAGAGGAAAACCAACCGTTTGGAGAAGCAAGCCCGGTTCCGGCTCGCCGGACAG
GTGGAGCAGCTCGACTATCGGGCGGGTCGAGGCGTGAGCCAAGCGCAGATCCGCCAACTGCTGGAAGGACACTGGCTGAGTCATCAACAAAATCTGCTGC
TGACGGGCGCGGCAGGCTGTGGCAAGAGTTACCTGGCGTGTGCTCTGGGTCGTTACTTTATCCGGCAAGGAGTGGGCGTGCGGTATTACCGGCTGAAAAC
CCTGCTCGAAGAGATGCGACTGGCACAAGCGGATGGCAGCTATCCGAAACTGCTGGAGCAAGTAAGTCACATAGGAATGTTGATCCTGGATGACTGGGGC
ATGGAGATGCTGGACGCGAGCGAACGCAGCAACCTGCTGGAGATCATCGATACCCGCTACGGCAAAGTCTCAACGATAGTGGCGAGCCAATTGCCGGTGG
AGAAGTGGTACGGAATGATCGGAGAGGCAACATTCGCCGAGGCAATCCTGGACCGATTGATCCACAGAGCAGTGCGCGTGACCTTAACGGGAGAATCGAT
GCGAAAACAGGGTGCCAACTTGACCGAAGCCGATCAGGCGGAGTAAAAGAAAGACGCTATAAACGCGGGCAGAAAAACGGTGATCGGCATGATGTTATTG
GCTGATCGACATGATGTTATTAGCCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1563 bp520 aa2051767+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSARPITVRKLKEILRLKYQAQLAHRQIALSLHISPSTVSYYLNRAAQLGILTWPLSESWDDVSLARAFLNTRAVHNTQHKPTPNWALLHQELKKTGQKG
VTLELLWQEYAERHSGSHYSYNHFCRLYKEWAALLQPSMRQTHIAGEKLFVDYCGQTMPIVDPHTGEILYRAQIFVAVLGASNYTFAEATRSQQLEDWVM
SHKRAFEFFGGVPQLVVPDCLKSAVSVARNTDPDLNPTYQMLAAHFGTAIMPARPRKPKDKSKAEVGVQIVTRWILAVLRHETFHSLAQLNQRMGELLTR
LNQKPFQKLPGCRASQFAEFDAPALKPLPDYPYHYTKVMLVLVGKDYHVDLEKHYYSVPHALQGKRLEAHLAEHTVTLYHAGKVVAEHPRSHRKGQHSSL
PAHMPAHHQALLEWSPERLLGWAVSIGPYTTHWVDGFIRNVAHPAQAVRPCLALLGQSKTYGKERLEAACLRGYLTGANRLHNIRSMLKNGLEQQPIASK
RQDPLQDIRHDNVHGEHYFH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17912546+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MQLHLNEQLSRLHLSGMKQALNQQQAQSMLYLDMGFEERLQLLLSHELVQREQRKTNRLEKQARFRLAGQVEQLDYRAGRGVSQAQIRQLLEGHWLSHQQ
NLLLTGAAGCGKSYLACALGRYFIRQGVGVRYYRLKTLLEEMRLAQADGSYPKLLEQVSHIGMLILDDWGMEMLDASERSNLLEIIDTRYGKVSTIVASQ
LPVEKWYGMIGEATFAEAILDRLIHRAVRVTLTGESMRKQGANLTEADQAE

 

Blast result :
Comments
ISAs28 is 68% (orfA, the transposase) and 74% (orfB) aa simiar to ISShfr5.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20