ISAs27

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas media WS
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 2628 bp

Ends


IR Length : 37/49

IRL : TGCATATTCCCACGCATGGCGATCGCTCAATAACATTCATGCCGATCACG
IRR : TGGCTAATAACATCATGTCGATCAGCCAATAACATCATGCCGATCACTTT

Insertion site


Left flankDirect repeatRight flankDR Length
CTACTCCGTAGCCAGGAACACAAAA5
CCCTTTATATCCTGACACGCTTGCC5
CCTGACCATCATCCCTACGAGCTGT5
GCCCAGCACAGTTGGCGCTGGGCGC5
TCGGCAGCCAGCTGGCTTGCAGTGA5
CTCAAACATGAAGAAATGAAATGAC5
TGAACCTTGACGTCATACATCCCCA5
ATCATCTGGCCATGCGACTCATTGAG6
CCCACCAGCTCACATTCGAAGGAGTA6
CCTCAGCTTGTTTGAGGATGGCGAT5
GTACGCCTCAACTGGCGCGACATTC5
AGCCTCAGCTTGTTTGAACTGGCGCGACATTCGC0
TGGTACGCCTCAACTGGTTTGAGGATGGCGATGA0
TGTCTCAGCCGATGCCGGCTACCAA5
CGAAGTGGTGCTCGTCGATGAGCTCA6
TTTGTCTCAGCCGATGCCATACTCTTTGCCAGAC0
ACGAAGTGGTGCTCGTCGATGCCGGCTACCAAGG0
CACTGAGTTGTGCCCTCCTCGTCGATGAGCTCAC0

DNA sequence

TGCATATTCCCACGCATGGCGATCGCTCAATAACATTCATGCCGATCACGTAATAACATCCATGCCGATCACTCAATAACATCCCAAGCCGATCACTTTT
TACCCTCGATGGGAAACCATGATCGGCATCCATGTTATTGGTGACTTTTTCTTCCCGCTTCGTTAAACCCGCACTTTCCCATCTGCTCCAACAGAGGAAC
GTGCATGTCTGCGAGGCCTATCACTGTGAGAAAACTCAAAGAAATTCTTCGCCTTAAATTTCAGGCTCAACTGGCTCATCGGCAGATCGCCCTCAGCCTG
CATATCTCTCCTAGCACCGTATCCTATTACCTCAACCGGGCGGCTCAACTGGGTATCATGACCTGGCCCCTGTCTGAGTCCTGGGATGATGTCTCCCTGG
AGCGCGCATTTCTCAAGACCTGCTCTGTTCATAAAACTCAGCACAAACCCACTCCCAACTGGGCCTTGTTGCATCAGGAACTCAAAAAACCAGGTCAAAA
AGGCGTGACCCTCGAGCTGCTATGGCAGGAGTACGCCGAGCGCCATCCAGGCTCCCACTACAGCTACAACCACTTCTGTCGGCTCTATAAAGAGTGGGCG
GCGCTGCTGCAACCCTCGATGCGGCAGACCCATATTGCGGGCGAAAAACTCTTTGTCGACTACTGCGGCCAGACCATGCCCATTGTCGATCCGCATACCG
GCGAGATCCTTTACCGGGCCCAGATCTTTGTCGCCGTGCTGGGGGCCTCCAACTACACCTTTGCCGAGGCCACCCGCTCCCAGCAGCTCGATGACTGGGT
GATGAGCCACAAGCGCGCTTTCGAGTTCTTTGGCGGTGTGCCGCAATTGGTGGTACCTGATTGCCTCAAGAGTGCCGTCAGCGTGGCGCGCAATACCGAT
CCGGATCTCAACCCTACCTACCAGATGCTGGCGGCCCATTTCGGTACGGCGATCATGCCCGCGCGGCCCCGCAAGCCGAAGGATAAGTCCAAGGCGGAGG
TGGGCGTGCAGATCGTCACCCGCTGGATCCTGGCGGTGCTGCGTCATGAAACCTTCCATAGCCTGGCCCAGCTCAATCAGCGAATGGGTGAACTGCTGAC
CCGGCTCAACCAGAAATCCTTCCAGAAGCTGCCTGGCTGCCGTGCCTCACTGTTTGCCGAGCTCGATGCACCGGCGCTCAAGCCGTTGCCGGATTATCCC
TATCACTACACCAAAGTAGTGCCGGTGCTGGTTGGTAAGGACTACCACGTCGATCTGGAAAAGCACTATTACTCAGTGCCCTATGCCTTGCAGGGCAAGC
GGCTGGAGGCGCATCTGGCCGAGCATACCGTCACGCTGTACCACGCGGGCAAGATCGTGGCCGAACACCAGCGGAGTCATCACAAAGGCCAGCACAGCAG
CCTGCGTGAACACATGCCCGCGCATCATCAGGCACTGCTGGAGTGGTCGCCGGAGCGACTGCTTGGCTGGGCGGCGTCCATCGGCCCCTACACCACGCAT
TGGGTGGATGGCTTTATCCGGCAGGCAGAGCATTCGACGCAAGCGGTGCGCCCTTGCCTGGCCTTGCTGGGGCAGAGCAAAACCTATGGCAAGGAGCGGC
TGGAAGCGGCCTGCCTCCGGGGGCACCTGACCGGCGCCAACCGGCTGCACAACATCCGCAGCATGCTCAAGCATGGCCTGGAGGAGCAACAACTCATTGC
CAACGAGCGACAGGATCCGCTGCAAGACATCCGTCATGACAATGTCCATGGCGAGCACTACTTCCACTAACCCCACGGATCAGGAGAAGCCGATGCAATT
ACACCTCGATGAACAACTAAGCCGCCTGCACTTGAGCGGCATGAAACAGGCTCTGAACCAGCAGCAGGCCCAGAGCATGCTGTACCTGGATATGGGTTTT
GAAGAGCGGCTGCAACTATTACTGAGCCATGAGCTGGTGCAGCGGGAGCAGAGGAAAACCAACCGCCTGGAGAAGCAAGCCCGGTTCCGGCTAGCCGGAC
AGGTGGAGCAGCTCGACTATCGGGCGGGTCGAGGCGTGAGCAAAGCGCAGATCCGCCAACTGCTGGAAGGACACTGGCTGAGTCATCAACAAAACCTGCT
GCTAACGGGCGCGGCAGGCTGTGGCAAGAGTTACCTGGCGTGTGCCCTGGGCCGTTACTTTATCCGGCAAGGAGTGGGCGTGCGGTATTACCGGCTGAAA
ACCCTACTTGAAGAGATGCGACTGGCCCAAGCGGATGGCAGCTATCCGAAACTGCTGGAGCGGGTAAGCAACATAGGGGTACTGATCCTGGATGACTGGG
GCATGGAGATGCTGGACGCGAGCGAACGCAGCAACCTGCTGGAGATCATCGATACCCGCTACGGCAACGTCTCAACGATAGTGGCGAGCCAATTGCCGGT
GGAGAAGTGGTACGGGATGATCGGAGAGGCAACCTTCGCCGAGGCGATCTTGGACCGACTGATCCACAGAGCGGTGCGCGTGACCTTAACGGGAGAATCG
ATGAGAAAACAGGGCGCCAACTTGACCGATGCCGATCAGGCGGAGTAAAAGGAAGACGCTATAAACGCGGGCAGAAAAAAAGTGATCGGCATGATGTTAT
TGGCTGATCGACATGATGTTATTAGCCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1563 bp520 aa2051767+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSARPITVRKLKEILRLKFQAQLAHRQIALSLHISPSTVSYYLNRAAQLGIMTWPLSESWDDVSLERAFLKTCSVHKTQHKPTPNWALLHQELKKPGQKG
VTLELLWQEYAERHPGSHYSYNHFCRLYKEWAALLQPSMRQTHIAGEKLFVDYCGQTMPIVDPHTGEILYRAQIFVAVLGASNYTFAEATRSQQLDDWVM
SHKRAFEFFGGVPQLVVPDCLKSAVSVARNTDPDLNPTYQMLAAHFGTAIMPARPRKPKDKSKAEVGVQIVTRWILAVLRHETFHSLAQLNQRMGELLTR
LNQKSFQKLPGCRASLFAELDAPALKPLPDYPYHYTKVVPVLVGKDYHVDLEKHYYSVPYALQGKRLEAHLAEHTVTLYHAGKIVAEHQRSHHKGQHSSL
REHMPAHHQALLEWSPERLLGWAASIGPYTTHWVDGFIRQAEHSTQAVRPCLALLGQSKTYGKERLEAACLRGHLTGANRLHNIRSMLKHGLEEQQLIAN
ERQDPLQDIRHDNVHGEHYFH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17932548+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MQLHLDEQLSRLHLSGMKQALNQQQAQSMLYLDMGFEERLQLLLSHELVQREQRKTNRLEKQARFRLAGQVEQLDYRAGRGVSKAQIRQLLEGHWLSHQQ
NLLLTGAAGCGKSYLACALGRYFIRQGVGVRYYRLKTLLEEMRLAQADGSYPKLLERVSNIGVLILDDWGMEMLDASERSNLLEIIDTRYGNVSTIVASQ
LPVEKWYGMIGEATFAEAILDRLIHRAVRVTLTGESMRKQGANLTDADQAE

 

Blast result :
Comments
ISAs27 is 68% (orfA, the transposase) and 70% (orfB) aa simiar to ISEc12.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20