ISAs22
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP022426 | ND | Aeromonas salmonicida | Aeromonas media WS Aeromonas salmonicida Aeromonas salmonicida subsp. pectinolytica 34mel |
DNA section
IS Length : 1198 bp
Ends
IR Length : 12/12
IRL : GGTAATCCCCCCGAAAAACTGAGGTGCTCATAAGTAGAATTTTCTCGTAT
IRR : GGTAATCCCCCCATTTTTTGGAGCACTCAAAAGTAGAGGTCATGCGGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTCTCCATGTAG | GAGG | CACGCGCTATGA | 4 |
| CAGTTTTCAAGC | CATT | GACTTGAAAAGA | 4 |
DNA sequence
GGTAATCCCCCCGAAAAACTGAGGTGCTCATAAGTAGAATTTTCTCGTATCTTGAACGCAGGAGGTTCTACATGAAAACATCACGCTTTAGCGATGCACA
GATCATCGCCATCCTCAAGCAGGCCGAGTCTGGTTCACCCGTCCCTGAGCTCTGCCGTGAGCATGGCATCAGCTCCGCCACCTTCTACAAGTGGCGCTCC
AAATTCGGTGGCATGGATGCCTCTCTCATGGCGCGCCTCAAAGAGCTGGAAGAGGAAAATCGTCGCCTTAAGAAGATGTATGCCGAGGAGCGCCTAAAGG
CTGAGATCATCTCCGAGGCGATGGCAAAAAAGTGGTGACGCCATCTGCCCGCAGGGAGATGGCGACCAAGGCTGTGGCTCATTACGCCATTTCTATCCGT
CTGGCTTGCCAGGTGTTCAAGGTGAGCGAGGGTTGCTACCGTTATCAGCCCGTGCTGGCTGATGAAAACCAGGAGATTGCCGACTGGCTGCTGCGCCTCA
CCGCCAACCAGCGCAACTGGGGTTTCGGACTGTGCTTCCTGTACCTGCGTAACGTCAAAGGCTTTGGCTGGAACCACAAGCGGGTCTACCGGATCTATCG
GGCGCTAGAGCTCAATCTGCGGATAAAACCCAAGAAGCGGCTAGTGCGGGCCAAGCCGGAGCCGTTGGCAGTCCCTGACCAACCGAATCACTGTTGGTCG
ATGGACTTTATGCATGACCAGCTCAGCGATGGTCGTTCGGTGCGGTTGTTTAATGTGATCGATGACTTCAATCGCGAAGCACTGTGCATCGAGGTGGATT
TTTCCTTGCCTGCCTCGCGAGTCAAACGGGTGCTGGAGCAGGTCATCGAGTGGCGGGGCAAACCAGCCGCAATCCGCTGTGATAATGGTCCGGAATACAT
CAGCGAAGAGCTCAAGCAATGGGCGCAGCAGAAGAGGATTGCGCTGAGGTATATCCAGCCAGGCAACCCGCAGCAGAATGCCTACATAGAGCGATTTAAC
CGCACTGTGCGTTACGACTGGTTGGGCCATTACCTGTTTGAGTCACTGAATGAGTTACAGGAATTTGCCACGAACTGGCTGTGGGTTTATAACCATGAGC
GGCCCAATATGGCGCTGGGCGGCTACACGCCGAAACAGCGCTTGGCACAGGCCGCATGACCTCTACTTTTGAGTGCTCCAAAAAATGGGGGGATTACC
GATCATCGCCATCCTCAAGCAGGCCGAGTCTGGTTCACCCGTCCCTGAGCTCTGCCGTGAGCATGGCATCAGCTCCGCCACCTTCTACAAGTGGCGCTCC
AAATTCGGTGGCATGGATGCCTCTCTCATGGCGCGCCTCAAAGAGCTGGAAGAGGAAAATCGTCGCCTTAAGAAGATGTATGCCGAGGAGCGCCTAAAGG
CTGAGATCATCTCCGAGGCGATGGCAAAAAAGTGGTGACGCCATCTGCCCGCAGGGAGATGGCGACCAAGGCTGTGGCTCATTACGCCATTTCTATCCGT
CTGGCTTGCCAGGTGTTCAAGGTGAGCGAGGGTTGCTACCGTTATCAGCCCGTGCTGGCTGATGAAAACCAGGAGATTGCCGACTGGCTGCTGCGCCTCA
CCGCCAACCAGCGCAACTGGGGTTTCGGACTGTGCTTCCTGTACCTGCGTAACGTCAAAGGCTTTGGCTGGAACCACAAGCGGGTCTACCGGATCTATCG
GGCGCTAGAGCTCAATCTGCGGATAAAACCCAAGAAGCGGCTAGTGCGGGCCAAGCCGGAGCCGTTGGCAGTCCCTGACCAACCGAATCACTGTTGGTCG
ATGGACTTTATGCATGACCAGCTCAGCGATGGTCGTTCGGTGCGGTTGTTTAATGTGATCGATGACTTCAATCGCGAAGCACTGTGCATCGAGGTGGATT
TTTCCTTGCCTGCCTCGCGAGTCAAACGGGTGCTGGAGCAGGTCATCGAGTGGCGGGGCAAACCAGCCGCAATCCGCTGTGATAATGGTCCGGAATACAT
CAGCGAAGAGCTCAAGCAATGGGCGCAGCAGAAGAGGATTGCGCTGAGGTATATCCAGCCAGGCAACCCGCAGCAGAATGCCTACATAGAGCGATTTAAC
CGCACTGTGCGTTACGACTGGTTGGGCCATTACCTGTTTGAGTCACTGAATGAGTTACAGGAATTTGCCACGAACTGGCTGTGGGTTTATAACCATGAGC
GGCCCAATATGGCGCTGGGCGGCTACACGCCGAAACAGCGCTTGGCACAGGCCGCATGACCTCTACTTTTGAGTGCTCCAAAAAATGGGGGGATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSRFSDAQIIAILKQAESGSPVPELCREHGISSATFYKWRSKFGGMDASLMARLKELEEENRRLKKMYAEERLKAEIISEAMAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 855 bp | 284 aa | 305 | 1159 | + | No |
Description : Second part of the transposase
ORF sequence :
DHLRGDGKKVVTPSARREMATKAVAHYAISIRLACQVFKVSEGCYRYQPVLADENQEIADWLLRLTANQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRA
LELNLRIKPKKRLVRAKPEPLAVPDQPNHCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRVLEQVIEWRGKPAAIRCDNGPEYIS
EELKQWAQQKRIALRYIQPGNPQQNAYIERFNRTVRYDWLGHYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
LELNLRIKPKKRLVRAKPEPLAVPDQPNHCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRVLEQVIEWRGKPAAIRCDNGPEYIS
EELKQWAQQKRIALRYIQPGNPQQNAYIERFNRTVRYDWLGHYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 72 | 1159 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSRFSDAQIIAILKQAESGSPVPELCREHGISSATFYKWRSKFGGMDASLMARLKELEEENRRLKKMYAEERLKAEIISEAMAKKVVTPSARREMATK
AVAHYAISIRLACQVFKVSEGCYRYQPVLADENQEIADWLLRLTANQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRALELNLRIKPKKRLVRAKPEPLA
VPDQPNHCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRVLEQVIEWRGKPAAIRCDNGPEYISEELKQWAQQKRIALRYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
AVAHYAISIRLACQVFKVSEGCYRYQPVLADENQEIADWLLRLTANQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRALELNLRIKPKKRLVRAKPEPLA
VPDQPNHCWSMDFMHDQLSDGRSVRLFNVIDDFNREALCIEVDFSLPASRVKRVLEQVIEWRGKPAAIRCDNGPEYISEELKQWAQQKRIALRYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQEFATNWLWVYNHERPNMALGGYTPKQRLAQAA
Blast result :
Comments
ISAs22 is 96% aa similar to ISKpn10.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20