ISAs25

  • Family IS1634
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas media WS
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1722 bp

Ends


IR Length : 20/21

IRL : CCGTCATTCCGCACCTGATTTTGCTATTTTCAGTGCTTGACGGCGATCGG
IRR : CCGACATTCCGCACCTGATTTAGGAATAAATTTGCCGATAGCGCTCACCC

Insertion site


Left flankDirect repeatRight flankDR Length
ATAATTAAAACTTTAGAGCTCTACCTCACT6
TCATGACCATTATTATAGGGGGAGGAGGGG6

DNA sequence

CCGTCATTCCGCACCTGATTTTGCTATTTTCAGTGCTTGACGGCGATCGGCCGTTCTGATCTATTACTCTCTTTTGCAGGTAGATCATCATGGCCGCACC
CGCTATCTCGATCCGTAACCTCGATCACCTTGGTTTGGTCGCCGCACTCTGCCAGGAACTGGGCATTGCCCGCATGATCGATGCCTTGCTCCCCAAAACC
CCGCCTTTCAAGGTCTCACATGGTGAAGCCTTGGTCGCCATGATAGTGAACGGCCTCGGCTTTCACAGCAGCACCCTGCACATGTTCCCGCAATTCTTCG
CTAACAAGCCGGTTGAGCGCCTGATTGGCCCCGGGATCTGTGCTGATGACCTCAATGATGATGTGCTCGGTCGCTGTCTCGATGCCCTCTTTGAGGCCGA
TGTCAGCGCCTTGTATCAGGTGATGGCGGCGCAAGTTGTCGAGCGGCTGGGGCTCAAAAGCACCGCAGTGCACCTCGATATTACCAGCTTCCACGTCGAT
GGCGCCTATGACTGTGCCGATGGGGATCTGGTCGGTAAGCTGCAACTGGTGCGTGGTTATAGTCGTGATCACCGGCCAGAGCTGAATCAAGTGATCCTTG
AGCTCATCTGTGAAAATCAGGCCGGCCTACCTGTCTATATGCAGGCCCTGAGCGGCAATAGTAATGACACCAAAGCCTTTGCCCAGACCGTTCGTCGCCA
TTTGAACAGTCTCAAGGCCGCGCAGGAGTGCCGTTATCTGGTCGGCGATGCCGCACTGTACTGCGCCGATACCTTGCAATTACTGGCGCAACAGCAGCAA
CTATTTGTGACCCGGGTGCCCGTCACTCTCAATGAGGCAAAGCAGGCCGTGGCAACCATAGGTGCACAACCGCTGACCGCTCTGGGCAATGGCTATCATG
GCCGTTGGCAACACGCGAATTATGCCGGGGTCGCGCAACGTTGGTTGCTGGTGCGCAGCGAACAGGCCAGCCACCGCGAGCAGCAGACATTGGCCAAAAA
CCTGCTCAAAGACAGTACCCGCGAGCTCAAAGCCTTCGCCAAATTGTGTGCCCGACGCTTTGCTTGTGAGGCGGATGCCCAAGCTGAGTTGAGCTGTTTT
ACCGCCAGCTTGATGCTGCTGCAACTGGATGCCGAAGTGGTAGGTGAACCCGTTTATACAGGACGTGGCAGACCCAAGCGTGGCGAAGAGCCAATTGGCC
ATCAGTTTCAAATCACTGGGTTGGCGGCAACTAGCCTGGCTTGCGTTGAAGAGGCCCGCAACCAGACCGGGGTGTTCATCCTGGCGACCAATGACCACAG
CGATACGCTGACCATGGCCGAACTGTTGGCCACCTATAAAGCGCAGCAGAATGTCGAACGGGGCTTTCGTTTTCTCAAGAGCCCGGAGTTTTTAACCTCC
TCACTGTACCTGAAAAAGCCGGAGCGGATTGAAGCGTTGCTCATGGTGATGACCTGTAGCCTGATGATTTACGCAGCGTTGGAGCACCGGATCCGGCAGG
GATTAGTGGAGCAGAATCGCAGTGTGGCTGATATGAAAAAGAAACCGACCCAGCAGCCGACGGCCCGTTGGATCTTTTTGCGCTTTGGTGGCATCCATGA
ATATCGACTGGGAGAAGCTCCGCCACAAGTAACGGAGCTAACAGGAGACCAGCAGATCATTTTGGAGGTATTGGGTGAGCGCTATCGGCAAATTTATTCC
TAAATCAGGTGCGGAATGTCGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1614 bp537 aa901703+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAAPAISIRNLDHLGLVAALCQELGIARMIDALLPKTPPFKVSHGEALVAMIVNGLGFHSSTLHMFPQFFANKPVERLIGPGICADDLNDDVLGRCLDAL
FEADVSALYQVMAAQVVERLGLKSTAVHLDITSFHVDGAYDCADGDLVGKLQLVRGYSRDHRPELNQVILELICENQAGLPVYMQALSGNSNDTKAFAQT
VRRHLNSLKAAQECRYLVGDAALYCADTLQLLAQQQQLFVTRVPVTLNEAKQAVATIGAQPLTALGNGYHGRWQHANYAGVAQRWLLVRSEQASHREQQT
LAKNLLKDSTRELKAFAKLCARRFACEADAQAELSCFTASLMLLQLDAEVVGEPVYTGRGRPKRGEEPIGHQFQITGLAATSLACVEEARNQTGVFILAT
NDHSDTLTMAELLATYKAQQNVERGFRFLKSPEFLTSSLYLKKPERIEALLMVMTCSLMIYAALEHRIRQGLVEQNRSVADMKKKPTQQPTARWIFLRFG
GIHEYRLGEAPPQVTELTGDQQIILEVLGERYRQIYS

 

Blast result :
Comments
ISAs25 is 94% aa similar to ISShes12.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20