ISAs24

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1394 bp

Ends


IR Length : 16/21

IRL : ---ttAATGTGATGGTCAGCCTGACCCACAGCCCAGCAGGCTACGCTTGC
IRR : cataaaAATCTATGGTCAGCCTGAACTTTGCAAGGCCAGATTGATGATGA

Insertion site


Left flankDirect repeatRight flankDR Length
AGTGCCGGGTGCTGGAATTC0
TATCCCGGGAGTGGCGTTTA0
TGCCGGCGGCGTCTGCAAAA0
TATCGCGGGTGTGCAGAGAC0
TATCGGGGGAGCTGCCATTC0
CAGGCCGGGTTCTATTGCAA0
CTTGGCCGGTGGCAGAGGCG0
GATTGCCGGTGCCGCCGCAC0
TGCGGGGCGTGCAGAAATGA0
CCAGGCCGGGTCTGGCCCGC0

DNA sequence

TTAATGTGATGGTCAGCCTGACCCACAGCCCAGCAGGCTACGCTTGCTGGGCTGTTTCTTTTCTACTTCAGGAGAGCATCATGGCAACTATCGCACTCTT
TGGACTCGACATCGGCAAGCACTCCTTTCATCTCGTCGGACAGGATGCCAACGGCAAACCCGTCTTCAAACGCCAGTTCACTCGTTCTAGCCTCATTCAT
TACCTTGCTCAGCACCCCGCTTGTCGCATCGTGATGGAAGCCTGCTGTGGCGCACACTGGCTAGCCCGTCAACTCGCTGGTTTCGGCCATCAGGTGCAAT
TGATCGCACCACAGTATGTTCGCCCTTTTGTCACCGGCAACAAGAACGACTTCCTTGATGCACACGCCATCTGTGAAGCGGCCTCCCGCCCCTCCATGCG
CTATGTCGGCGTCAAAACACCCGAACAGCAGGCCAGCTCGGCACTGCATCGACTGCGCGAAGCCCGTATTGCCGACCGGGTGCAAACCGCTAATCAAATC
CACGCCCTGCTGCTCGAGTTTGGCATTGTGCTGCCGCTAGGGGCACGGGGCATCAAGCAAGTCCCTGATATCGTTATGAATGCTAGTACTGACCTCCCAC
ACGCTGCACGCCGGATCCTCTCGGCACAGTATGAGCACTATCGCTACCTGGCAGAGTTGATTGCCGGGCTGGATAGCCAGATTGGAGCGCAAGCCAAGGC
CGATGCGTTGACCAGTCGATTGATGACCATTCCCGGCATAGGCCCCATCACTGCAAGCCAGCTGGCTGCCGATGCAGGCAACGCGCAGAGCTATGGCTCG
GCACGCCATTTTGCCGCGTCCCTTGGGCTGGTACCGCGCCAACACTCCACGGGTGGTAAGAGCAAACTGCTGGGGATCAGCAAACGGGGGGACAAATCGC
TGCGGCGGTTACTGGTGCAGTGCGCTCGGGTGGTGATGCAGAACGCTGCCAAATGGCAAAGTGCGGTGGCGCAGTGGACGCAGCAGTTGATGACACGACG
GCACTCGAATGTGGTGGCCTGTGCGTTGGCCAACAAGCTGGCACGGATCGTATGGGCAGTGCTAGTGAAGGGGGAGGAGTATCGTCCGAACCCCAATGCT
GCCTGAAGGTAAGTAACACATCGCCGAGTTTCCCCGGCGACATGAAGTAACACGCCTGCTTTTGCGATGTTCAGAAAATGATGACGACAACTGGCCCAAC
GGCCTGAGGGTAAACCTGACATAAAAAACAGTCTTCAAGACTGACGGTTTTTTGAGGTCCCTCAGGCGCGGCTCACATCATGGATCGGGCATGATAGCCC
ACCAAGAATCCGGATAGATTAGAGCAAGCCTGCCCTGTCAGTACTCATCATCAATCTGGCCTTGCAAAGTTCAGGCTGACCATAGATTTTTATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1026 bp341 aa811106+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MATIALFGLDIGKHSFHLVGQDANGKPVFKRQFTRSSLIHYLAQHPACRIVMEACCGAHWLARQLAGFGHQVQLIAPQYVRPFVTGNKNDFLDAHAICEA
ASRPSMRYVGVKTPEQQASSALHRLREARIADRVQTANQIHALLLEFGIVLPLGARGIKQVPDIVMNASTDLPHAARRILSAQYEHYRYLAELIAGLDSQ
IGAQAKADALTSRLMTIPGIGPITASQLAADAGNAQSYGSARHFAASLGLVPRQHSTGGKSKLLGISKRGDKSLRRLLVQCARVVMQNAAKWQSAVAQWT
QQLMTRRHSNVVACALANKLARIVWAVLVKGEEYRPNPNAA

 

Blast result :
Comments
ISAs24 is 73% aa similar to ISHar6.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20