ISAs21

  • Family IS66
  • Group ISBst12
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas media WS
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1530 bp

Ends


IR Length : 23/29

IRL : GTAACTGTTCACCCAGATGGCATTGACAGGAAAAAGTGAGACGATCATCC
IRR : GTAACCGTTCACCGGGACGGTGTTGACAGCACCAACTCCTCCGGGAACGG

Insertion site


Left flankDirect repeatRight flankDR Length
TTTCTACTCCATCATCAACTCTAGTTTG8
GATCCAGCCAGTCCAGGTTGCCCCCGGT8
CCACGCTATTACCTCCCGGA0

DNA sequence

GTAACTGTTCACCCAGATGGCATTGACAGGAAAAAGTGAGACGATCATCCCTTGATCCACTGTTGCCCCTTGCCTGATCCCGCCAGCTTGGCACCATCAC
GTCATGAGCAAACCCATTACGCCACCACCCGGCCTGCCGCTGACCCTCGAGCAAGCCAACGAGCTGATTTACAAGCTGATGGCGCGCATCGCCGAGCTTG
AAGACCGCCTCAACCAAAACAGCAATAACTCCTCCAAGCCCCCCTCCACTGATGGCTCGGGGACCCCGCCTGCGCGCAAGCGCCCCGCTTCCGGCAAGGC
TCGTGGTGCCCAGCCTGGCCACAAGGGGCAGCGGCGTGAACGTCATCCTGAGGACGACCGACTGACGGCAATACCACACTATCCTGCCCGGCAGTGCGCC
TGCTGTGGTGAGCAGGTGGTGTCCCATGACAAGCCCTACCGGGTTCATCAGGTGTTTGACCTGCCCGAGGTCAGCTATGTGGTGACAGAGCATCAGCTGT
ATCGGGGCACCTGCCGTGGGTGCGTCACCACCAGTGAAGCAGCCCTGCCCGATACGGTGAGCCACAGCCAGATGGGGGCTAATCTGCTGAGCTATATCGC
TTTGCAGAGTGGTCAGTTCCACCAGAGCATCAGCAAGATACAGCAGCAGCTCAAGCAGCACTTCGGTTTGAGCTTTAGCCGAGGCGCCATCAGTGAAGCG
CAAGGCCGGGTCAGCGCCATGCTCACCCCGACCCACCAAGCCATCAAACAGCAAGTGCAGTCTGCCGCCATCATCCATGCCGATGAAACCCGGCACCAGC
GGGGCGGTGAGCGACGCTGGATGTGGCTGGCCCTGAGCAAGGTCGCGGTCTGCTTCATGACGGCGTTTGGCCGCGGTCAGGAGGCAGCGAAGCGGTTGCT
GGGCAGTGAGCTTGATGGGGTGCTGGTGACCGACCAGTATGCGGGATACCGCTTCATCGACAGCGCCCAGCGCCAACTGTGCTGGGCGCATGTGCTGCGC
AACGTGGCGGCGATTGCCGAGAGTAGCGACGCCGTCAACCAGCCTATCGGAGCCCGGCTGGTACTGCTGGCTGACACGGTGTTCAGAACCCGGCATGGCT
ATGAACAAGGCTGCTTAAGCCGGGAGCAGTACCAGCGAAGGTTGCAGCGATGTCGGCAAAGCTGGCGAAGGCAGCTGGAGCGGGGCAGTGTACTGTGTTC
CAAGCGCTACCGTGGGCGCTGCCAGTTACTGCTCAAGGACGATGAGATGCTATGGCGTTTTATGGAAAACGACGAGATAGCGCTGACCAACAATGAAGCG
GAGCGGGCGCTGCGTGGCTATGTGCTGTGGCGCAAGGGAAGCTATGGCGTCTGGTCGCATCGAGGCGAGCAGTTCAGGCAGCGTATCCTATCGTTGGTGG
AGACGGCCAAGCGATTGGGCCGCTGCCCGCAGGAGTGGCTGAGGGCAGTTGTCAGGTCCTGCATCGAGAAAACCGACTACCCGTTCCCGGAGGAGTTGGT
GCTGTCAACACCGTCCCGGTGAACGGTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1419 bp472 aa1041522+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKPITPPPGLPLTLEQANELIYKLMARIAELEDRLNQNSNNSSKPPSTDGSGTPPARKRPASGKARGAQPGHKGQRRERHPEDDRLTAIPHYPARQCAC
CGEQVVSHDKPYRVHQVFDLPEVSYVVTEHQLYRGTCRGCVTTSEAALPDTVSHSQMGANLLSYIALQSGQFHQSISKIQQQLKQHFGLSFSRGAISEAQ
GRVSAMLTPTHQAIKQQVQSAAIIHADETRHQRGGERRWMWLALSKVAVCFMTAFGRGQEAAKRLLGSELDGVLVTDQYAGYRFIDSAQRQLCWAHVLRN
VAAIAESSDAVNQPIGARLVLLADTVFRTRHGYEQGCLSREQYQRRLQRCRQSWRRQLERGSVLCSKRYRGRCQLLLKDDEMLWRFMENDEIALTNNEAE
RALRGYVLWRKGSYGVWSHRGEQFRQRILSLVETAKRLGRCPQEWLRAVVRSCIEKTDYPFPEELVLSTPSR

 

Blast result :
Comments
ISAs21 is 91% aa similar to ISUnCu16.
ISAs21 is found in 28 copies in Aeromonas media WS (CP007567).
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20