ISAs19
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP022426 | ND | Aeromonas salmonicida | Klebsiella pneumoniae Aeromonas salmonicida subsp. salmonicida A449 Aeromonas salmonicida Aeromonas hydrophila YL17 Aeromonas salmonicida subsp. pectinolytica 34mel |
DNA section
IS Length : 1198 bp
Ends
IR Length : 22/33
IRL : TGTATGGAGATGGTATTCACCTGACAGTTGCCCACAAAAGGGAGTGGTCA
IRR : TGTACAGATCTAGTTATCACCTGACACCGGCCTCAAACCGGTGTCTTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACCATGGCC | ATCTTCC | AGATGTGAAA | 7 |
| CAACTCTCTC | TATAAACTTA | 0 | |
| GTAAGGGAAA | TATAAAC | AAAAGAGTTC | 7 |
| TAACTATTCG | TTTGGTG | TTTATTGGTT | 7 |
| GTAAGGGAAATATAAAC | TTTGGTGTTTATTGGTT | 0 | |
| TAACTATTCGTTTGGTG | TATAAACAAAAGAGTTC | 0 | |
| TGCTCCAGAA | CTTCGTT | GTTATGCAGA | 7 |
DNA sequence
TGTATGGAGATGGTATTCACCTGACAGTTGCCCACAAAAGGGAGTGGTCATACGGCTTCAAACCGGATAACAAAACGGTACCCCTACCGTGTTATCTGGA
GTCACCGTATGACCACCAACGAGAAAGTAGCACGTCGCAAACTCAGCCTGCTAGAACTCGCCAAAGAGCTCAATAACGTCAGCAAAGCCTGCAAGCTCAT
CGGCTACAGTCGTCAGCAGTTCTACGAAATCCGTCGTAATTACCAGACCTATGGCGCCGAGGGCTTGCTCGACAAACTGCCCGGCTGTAAAGGGGCTCAT
CCCAACCGGGTCGCTCCCGAGATTGAGCAGGCCATCCTCGATTATTCCCTCACCAGGCCTACTCACGGCCCGCTGCGGGTCGCGCAAGAACTAGCTCTAC
AAGGTATCAACGTCAGTGCCGGCGGCGTGCGTGGCGTGTGGCAACGTCACGATTTGCTCTCCAAACACGACCGCTTGTTACGTCTCGAGAAAACCCATCG
GGAACAAACGATTGAGCTCAATGACGAGCAAATTCGCCTGCTCGAGCGCTTCAGTCCCGAGTTCCGAGAACGTCAGATAGAGGTCCACTACACCGGGGAA
CTGGTGGCGGTCGACACCTTCTTCGTCGGCGCGCTCAAGGGGGTCGGTAAGGTGTATCTGCAAACCGTGCTGGACTGCTATAGCCGCCACGCCTGGGGGC
GGCTCTACACCAGCAAGCTGCCCATCACCTCGGTCCATGTGCTCAACGAAACGGTGCTGCCATTCTTTGAAACCCACGAGGCACGGGTCTATACCATCCT
GTCGGACAACGGGCGCGAGTTCTGCGGGCGCCCTGACCATCATCCCTACGAGCTGTTCCTGCAACTGGAGGGGATTGAGCACCGGACAACCAAGGTGCGC
AGGCCGCAGAGCAACGGCTTTATCGAGCGGCTACATCGAACCTTGCTGGATGAACACTTCCGTATCAAGGGGAGAACGACTTGGTATGAGTCGGTGGAGC
AGATGCAGACAGACCTGGATAGCTATCTGGAACACTACAACACCCAGCGGCCACATCAGGGCAGGATGATGGAGGGTCAGACCCCCTACAGCATGTTCAA
GAAGGGTCTGAAATTGATACCGAAGGAAGTGCGCACTAAAGTAGCGTAAACAAGACACCGGTTTGAGGCCGGTGTCAGGTGATAACTAGATCTGTACA
GTCACCGTATGACCACCAACGAGAAAGTAGCACGTCGCAAACTCAGCCTGCTAGAACTCGCCAAAGAGCTCAATAACGTCAGCAAAGCCTGCAAGCTCAT
CGGCTACAGTCGTCAGCAGTTCTACGAAATCCGTCGTAATTACCAGACCTATGGCGCCGAGGGCTTGCTCGACAAACTGCCCGGCTGTAAAGGGGCTCAT
CCCAACCGGGTCGCTCCCGAGATTGAGCAGGCCATCCTCGATTATTCCCTCACCAGGCCTACTCACGGCCCGCTGCGGGTCGCGCAAGAACTAGCTCTAC
AAGGTATCAACGTCAGTGCCGGCGGCGTGCGTGGCGTGTGGCAACGTCACGATTTGCTCTCCAAACACGACCGCTTGTTACGTCTCGAGAAAACCCATCG
GGAACAAACGATTGAGCTCAATGACGAGCAAATTCGCCTGCTCGAGCGCTTCAGTCCCGAGTTCCGAGAACGTCAGATAGAGGTCCACTACACCGGGGAA
CTGGTGGCGGTCGACACCTTCTTCGTCGGCGCGCTCAAGGGGGTCGGTAAGGTGTATCTGCAAACCGTGCTGGACTGCTATAGCCGCCACGCCTGGGGGC
GGCTCTACACCAGCAAGCTGCCCATCACCTCGGTCCATGTGCTCAACGAAACGGTGCTGCCATTCTTTGAAACCCACGAGGCACGGGTCTATACCATCCT
GTCGGACAACGGGCGCGAGTTCTGCGGGCGCCCTGACCATCATCCCTACGAGCTGTTCCTGCAACTGGAGGGGATTGAGCACCGGACAACCAAGGTGCGC
AGGCCGCAGAGCAACGGCTTTATCGAGCGGCTACATCGAACCTTGCTGGATGAACACTTCCGTATCAAGGGGAGAACGACTTGGTATGAGTCGGTGGAGC
AGATGCAGACAGACCTGGATAGCTATCTGGAACACTACAACACCCAGCGGCCACATCAGGGCAGGATGATGGAGGGTCAGACCCCCTACAGCATGTTCAA
GAAGGGTCTGAAATTGATACCGAAGGAAGTGCGCACTAAAGTAGCGTAAACAAGACACCGGTTTGAGGCCGGTGTCAGGTGATAACTAGATCTGTACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1041 bp | 346 aa | 109 | 1149 | + | No |
Chemistry : DDE
ORF sequence :
MTTNEKVARRKLSLLELAKELNNVSKACKLIGYSRQQFYEIRRNYQTYGAEGLLDKLPGCKGAHPNRVAPEIEQAILDYSLTRPTHGPLRVAQELALQGI
NVSAGGVRGVWQRHDLLSKHDRLLRLEKTHREQTIELNDEQIRLLERFSPEFRERQIEVHYTGELVAVDTFFVGALKGVGKVYLQTVLDCYSRHAWGRLY
TSKLPITSVHVLNETVLPFFETHEARVYTILSDNGREFCGRPDHHPYELFLQLEGIEHRTTKVRRPQSNGFIERLHRTLLDEHFRIKGRTTWYESVEQMQ
TDLDSYLEHYNTQRPHQGRMMEGQTPYSMFKKGLKLIPKEVRTKVA
NVSAGGVRGVWQRHDLLSKHDRLLRLEKTHREQTIELNDEQIRLLERFSPEFRERQIEVHYTGELVAVDTFFVGALKGVGKVYLQTVLDCYSRHAWGRLY
TSKLPITSVHVLNETVLPFFETHEARVYTILSDNGREFCGRPDHHPYELFLQLEGIEHRTTKVRRPQSNGFIERLHRTLLDEHFRIKGRTTWYESVEQMQ
TDLDSYLEHYNTQRPHQGRMMEGQTPYSMFKKGLKLIPKEVRTKVA
Blast result :
Comments
ISAs19 is 86% aa similar to ISDvu4.
The last four insertion sites correspond to a pair of transposons which occur in opposite orientation. If the current genome configuration would have resulted from a genome inversion, each transposon would have had a 7 bp target duplication prior to the inversion. The genome assembly is definitely correct, so that in the genome as is, these transposons do not have a target duplication.
The last four insertion sites correspond to a pair of transposons which occur in opposite orientation. If the current genome configuration would have resulted from a genome inversion, each transposon would have had a 7 bp target duplication prior to the inversion. The genome assembly is definitely correct, so that in the genome as is, these transposons do not have a target duplication.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20