ISAs18
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP022426 | ND | Aeromonas salmonicida | Aeromonas media WS Aeromonas salmonicida Aeromonas salmonicida subsp. pectinolytica 34mel |
DNA section
IS Length : 1261 bp
Ends
IR Length : 14/19
IRL : CTTAACCATCCCCTCATATGAGCGAGGCGTGGCGCGGCTTCTGAGGTATG
IRR : CTCAGAGATCCCCTCATTTTTAGAGACTCAAATAATTCCAAGTGTGCGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCATTTTCC | AAACATTGC | ACGTGCCACG | 9 |
| CCATGGAACA | GGGTTCATC | ATGAGTCATC | 9 |
| CCCCTGAGTT | CCAGCCCCC | AGTCTGCTGC | 9 |
| GCGATGATGC | TCTCCAGAT | AACAACAACT | 9 |
| GACGGATCAC | CTCTGCGAT | AGCACTCATG | 9 |
| TGAATTCGCG | GGTAGTGATA | 0 | |
| CACACAACCT | CATTTTCCC | CTCACAAATC | 9 |
| ATGACGTCTTGT | AGCCAAAAC | ACCACCTGACGT | 9 |
| TTCACACAACCTCATTTTCCC | AGCCAAAACACCACCTGACGT | 0 | |
| ATGACGTCTTGTAGCCAAAAC | CATTTTCCCCTCACAAATCCC | 0 |
DNA sequence
CTTAACCATCCCCTCATATGAGCGAGGCGTGGCGCGGCTTCTGAGGTATGATCGCCATCCTTGACCAGCTAATACCCAACAGACTATCCCCATGTTTAAC
CACGAACTAATCCTCAACCACATCGACGATATATTTGGACAGGACATGCATGCCAAGCGTGTTCTTTCCCTCGCCAATGCTACCCTCGGGGTTATCGAGT
CAGGTTCACTGGCCATCCACGCCATCGGTAGTGGCCTCTCCTTGGCCAACGGTCTCGAACGTAAACATGCGGTTAAACAGGTGGATCGCCTGCTCACTAA
CACCAAGCTCAATGTCTGGAGCCTCTTTGACGATTGGGTGCCCTATGTGGTCGGTGAACGTACCGAGATCGTAGTCTCCATGGACTGGACCGAATTCGAT
GCCGATGATCACAGTACCTTGGTTATCAGCCTGCAAACCAACCACGGTCGCAGTACTCCGCTGCTGTGGAAAACCCATCGGAAATCGTTGCTCAAAGGGC
AACGCAATGCCCATGAGAAAGAGCTGCTCACCAAGCTCAAGCAGACGTTGCCAGAGGGTATCTTCGTGACGGTAGTAGCTGACCGCGGCTTTGGTTACAT
GGAGCTGTTCGAACGCCTGGAGCAAGAGCTAGGCTTTGCTTATCTCATTAGATTTAAAGGAAATGTTTTGGTTGGTTACCCTGGTGTAGAGCAGGTTCCA
GCTCGGGACTGGCTAACGCCGACCGGGCGCACCCGAACCCTCAAAGCGGTGGAGCTAACTGGGCAGCGACAGCCTGTCGAACGGGTCTATTGTTGTCATA
AGAGTGGCATGAAAGACGCGTGGTTTCTGGCCAGTAACCGGACCGACCTGAGCGCCGCCAAGGCGTTGCAACTATACGGCCAGCGCTGGGGTATCGAGAC
CAGTTTTCGTGATATCAAAGATTACAAATTCGGCATGGGTATGGGAGATGTGCACATCAGTAATCCGGTCCGGCGCGACCGACTGTTTTTATTCAGTGCC
CTGGCCATCGTCTTGCTGACGCTGCTGGGTAAGGCTGGAGACAGTGTCGGTCTAGAGAGAACGATCAAGGTCAATACCAGCAAGAGCCGAACCTATTCGT
TCTTTCGGCAAGGGGTCATCTATTACCAGTTGCTACCTAAAATGAAAGAAGCCAATGCGGTCTTGTTGATGGATAAATTTATCTACTACCTGAAGCAACA
CCGCTTATATACCCGCACACTTGGAATTATTTGAGTCTCTAAAAATGAGGGGATCTCTGAG
CACGAACTAATCCTCAACCACATCGACGATATATTTGGACAGGACATGCATGCCAAGCGTGTTCTTTCCCTCGCCAATGCTACCCTCGGGGTTATCGAGT
CAGGTTCACTGGCCATCCACGCCATCGGTAGTGGCCTCTCCTTGGCCAACGGTCTCGAACGTAAACATGCGGTTAAACAGGTGGATCGCCTGCTCACTAA
CACCAAGCTCAATGTCTGGAGCCTCTTTGACGATTGGGTGCCCTATGTGGTCGGTGAACGTACCGAGATCGTAGTCTCCATGGACTGGACCGAATTCGAT
GCCGATGATCACAGTACCTTGGTTATCAGCCTGCAAACCAACCACGGTCGCAGTACTCCGCTGCTGTGGAAAACCCATCGGAAATCGTTGCTCAAAGGGC
AACGCAATGCCCATGAGAAAGAGCTGCTCACCAAGCTCAAGCAGACGTTGCCAGAGGGTATCTTCGTGACGGTAGTAGCTGACCGCGGCTTTGGTTACAT
GGAGCTGTTCGAACGCCTGGAGCAAGAGCTAGGCTTTGCTTATCTCATTAGATTTAAAGGAAATGTTTTGGTTGGTTACCCTGGTGTAGAGCAGGTTCCA
GCTCGGGACTGGCTAACGCCGACCGGGCGCACCCGAACCCTCAAAGCGGTGGAGCTAACTGGGCAGCGACAGCCTGTCGAACGGGTCTATTGTTGTCATA
AGAGTGGCATGAAAGACGCGTGGTTTCTGGCCAGTAACCGGACCGACCTGAGCGCCGCCAAGGCGTTGCAACTATACGGCCAGCGCTGGGGTATCGAGAC
CAGTTTTCGTGATATCAAAGATTACAAATTCGGCATGGGTATGGGAGATGTGCACATCAGTAATCCGGTCCGGCGCGACCGACTGTTTTTATTCAGTGCC
CTGGCCATCGTCTTGCTGACGCTGCTGGGTAAGGCTGGAGACAGTGTCGGTCTAGAGAGAACGATCAAGGTCAATACCAGCAAGAGCCGAACCTATTCGT
TCTTTCGGCAAGGGGTCATCTATTACCAGTTGCTACCTAAAATGAAAGAAGCCAATGCGGTCTTGTTGATGGATAAATTTATCTACTACCTGAAGCAACA
CCGCTTATATACCCGCACACTTGGAATTATTTGAGTCTCTAAAAATGAGGGGATCTCTGAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1143 bp | 380 aa | 92 | 1234 | + | No |
Chemistry : DDE
ORF sequence :
MFNHELILNHIDDIFGQDMHAKRVLSLANATLGVIESGSLAIHAIGSGLSLANGLERKHAVKQVDRLLTNTKLNVWSLFDDWVPYVVGERTEIVVSMDWT
EFDADDHSTLVISLQTNHGRSTPLLWKTHRKSLLKGQRNAHEKELLTKLKQTLPEGIFVTVVADRGFGYMELFERLEQELGFAYLIRFKGNVLVGYPGVE
QVPARDWLTPTGRTRTLKAVELTGQRQPVERVYCCHKSGMKDAWFLASNRTDLSAAKALQLYGQRWGIETSFRDIKDYKFGMGMGDVHISNPVRRDRLFL
FSALAIVLLTLLGKAGDSVGLERTIKVNTSKSRTYSFFRQGVIYYQLLPKMKEANAVLLMDKFIYYLKQHRLYTRTLGII
EFDADDHSTLVISLQTNHGRSTPLLWKTHRKSLLKGQRNAHEKELLTKLKQTLPEGIFVTVVADRGFGYMELFERLEQELGFAYLIRFKGNVLVGYPGVE
QVPARDWLTPTGRTRTLKAVELTGQRQPVERVYCCHKSGMKDAWFLASNRTDLSAAKALQLYGQRWGIETSFRDIKDYKFGMGMGDVHISNPVRRDRLFL
FSALAIVLLTLLGKAGDSVGLERTIKVNTSKSRTYSFFRQGVIYYQLLPKMKEANAVLLMDKFIYYLKQHRLYTRTLGII
Blast result :
Comments
ISAs18 is 68% aa similar to ISAnsp7.
The last four insertion sites correspond to a pair of transposons which occur in opposite orientation. If the current genome configuration would have resulted from a genome inversion, each transposon would have had a 9 bp target duplication prior to the inversion. The genome assembly is definitely correct, so that in the genome as is, these transposons do not have a target duplication.
The last four insertion sites correspond to a pair of transposons which occur in opposite orientation. If the current genome configuration would have resulted from a genome inversion, each transposon would have had a 9 bp target duplication prior to the inversion. The genome assembly is definitely correct, so that in the genome as is, these transposons do not have a target duplication.
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20