ISAs17

  • Family IS3
  • Group IS2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas hydrophila ML09-119
Aeromonas hydrophila J-1
Aeromonas hydrophila pc104A
Aeromonas hydrophila AL09-71
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1333 bp

Ends


IR Length : 29/41

IRL : TAGATTGGCCCCTTGAAGATCCAGACACTTCGCCCCTCTTAAATTAAGAG
IRR : TGGATTTGACCCCATAGGTCCAGACACTTTTTCCCGCTTAGGGTTGTGAT

Insertion site


Left flankDirect repeatRight flankDR Length
GGGATGCCATGCCAGGTGGTGTTCC5
GTCATAGGCTCCATCCGCATAAACA5
ATAAGGTTGGTACTCTACCT0

DNA sequence

TAGATTGGCCCCTTGAAGATCCAGACACTTCGCCCCTCTTAAATTAAGAGGTAGTCTTGGAACTATGTTCAGTACTAGTCGCAATGAGAAGATGGTCGAA
GTGTTAACAGGGGCTGAGCGCCGTCGACGCCGGACTCCGCAGGAAAAGATCTCCATCGTGCAGCAGACCTTTGAGCCCGGTATGACCGTCTCTCATGTCG
CTCGCTTGCACGACGTCAACGCCAACCAGCTTTTCAAATGGCGTAAGCAATTCCAGGAAGGGTCTCTCACGGCGATTTCCGCCGGTGAGGATGTTGTTCC
AGCCTCAGAGTTGGCTGCTGCCATCAAGCAGATCCGAGAGCTCCAGCGTTTGCTCGGGAAGAAGACCATGGAGAACGAGATCCTTAAAGAAGCCGTGGAG
TATGGTCGAGCAAAAAAATGGATAGCGCATGCGCCCTTGTTGCCAGGGGACGACGATTAAGGGCTGTGAGCCAGGCCCTTAATGTATCGCGTGCGCAGCT
ATCCGTTCGTGTTCATCGAAAACCGGGTTGGCAAGATGGTCGTCATCATCGCCAGCGTGATGATGCAGCCTTACTTGGCCGTATCATGGAGGCGATGGCA
GAGCTTCCCTCCTATGGCTATCGCCGGGTCTGGGCGCTGTTACGGCGTCAGTCAGAGGCAACACGACAGCCTGTGGTAAACGCCAAGCGGGTATATCGCG
TCATGCGCGATCATGGCCTGCTGCTGGAAAGAAAACCGGCGGCACCATTGGCCCAGCAGGCACATAAGGGGCGTGTTGCCGTCAAAGAGAGCAATCGACG
TTGGTGCTCGGATGGCTTTGAATTTCGCTGTGATAACGGTGAAAAACTACGGGTCACGTTCGCCATGGACTGCTGCGATAGGGAAGCGTTGGACTGGGCT
GCCAGTACCGGGGGTTACGACAGTGACACGGTGCAACATGTCATGCTGAGGTCTGTCGAACGTCGCTTCGGGGATTCGTTGCCGGCATCACCGGTGGAAT
GGCTGACAGATAATGGTTCGGCATATCGAGCCCATGAGACCCGTGCGTTTGCACGAGAAATCGGGTTGGAGCCCAGAACGACTGCGGTGAGAAGCCCACA
GAGCAACGGTATAGCGGAGAGCTTCGTGAAGACGATGAAGCGAGACTATATCGAGATGATGCCAAAACCGGATAGCCGAACAGCGGTAGGCAATTTGGCT
ATCGCGTTTGAGCATTATAACGAGCACCACCCGCACAGCGCTTTGGGATACCGTTCACCCCGGGAATATCTGCGCAGTCGGGTATCACAACCCTAAGCGG
GAAAAAGTGTCTGGACCTATGGGGTCAAATCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
369 bp122 aa92460+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MVEVLTGAERRRRRTPQEKISIVQQTFEPGMTVSHVARLHDVNANQLFKWRKQFQEGSLTAISAGEDVVPASELAAAIKQIRELQRLLGKKTMENEILKE
AVEYGRAKKWIAHAPLLPGDDD

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
909 bp302 aa3881296+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RSRGVWSSKKMDSACALVARGRRLRAVSQALNVSRAQLSVRVHRKPGWQDGRHHRQRDDAALLGRIMEAMAELPSYGYRRVWALLRRQSEATRQPVVNAK
RVYRVMRDHGLLLERKPAAPLAQQAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCCDREALDWAASTGGYDSDTVQHVMLRSVERRFGDSLPA
SPVEWLTDNGSAYRAHETRAFAREIGLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNLAIAFEHYNEHHPHSALGYRSPREYLRSRVS
QP

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1205 bp401 aa921296+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVEVLTGAERRRRRTPQEKISIVQQTFEPGMTVSHVARLHDVNANQLFKWRKQFQEGSLTAISAGEDVVPASELAAAIKQIRELQRLLGKKTMENEILKE
AVEYGRAKKMDSACALVARGRRLRAVSQALNVSRAQLSVRVHRKPGWQDGRHHRQRDDAALLGRIMEAMAELPSYGYRRVWALLRRQSEATRQPVVNAKR
VYRVMRDHGLLLERKPAAPLAQQAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCCDREALDWAASTGGYDSDTVQHVMLRSVERRFGDSLPAS
PVEWLTDNGSAYRAHETRAFAREIGLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNLAIAFEHYNEHHPHSALGYRSPREYLRSRVSQ
P

 

Blast result :
Comments
ISAs17 is 84% aa similar to IS2.
ORFAB has been generated in silico, assuming a translational frameshifting mechanism (as described
for IS2).
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20