ISAs17
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP022426 | ND | Aeromonas salmonicida | Aeromonas salmonicida Aeromonas hydrophila ML09-119 Aeromonas hydrophila J-1 Aeromonas hydrophila pc104A Aeromonas hydrophila AL09-71 Aeromonas salmonicida subsp. pectinolytica 34mel |
DNA section
IS Length : 1333 bp
Ends
IR Length : 29/41
IRL : TAGATTGGCCCCTTGAAGATCCAGACACTTCGCCCCTCTTAAATTAAGAG
IRR : TGGATTTGACCCCATAGGTCCAGACACTTTTTCCCGCTTAGGGTTGTGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGATGCCAT | GCCAG | GTGGTGTTCC | 5 |
| GTCATAGGCT | CCATC | CGCATAAACA | 5 |
| ATAAGGTTGG | TACTCTACCT | 0 |
DNA sequence
TAGATTGGCCCCTTGAAGATCCAGACACTTCGCCCCTCTTAAATTAAGAGGTAGTCTTGGAACTATGTTCAGTACTAGTCGCAATGAGAAGATGGTCGAA
GTGTTAACAGGGGCTGAGCGCCGTCGACGCCGGACTCCGCAGGAAAAGATCTCCATCGTGCAGCAGACCTTTGAGCCCGGTATGACCGTCTCTCATGTCG
CTCGCTTGCACGACGTCAACGCCAACCAGCTTTTCAAATGGCGTAAGCAATTCCAGGAAGGGTCTCTCACGGCGATTTCCGCCGGTGAGGATGTTGTTCC
AGCCTCAGAGTTGGCTGCTGCCATCAAGCAGATCCGAGAGCTCCAGCGTTTGCTCGGGAAGAAGACCATGGAGAACGAGATCCTTAAAGAAGCCGTGGAG
TATGGTCGAGCAAAAAAATGGATAGCGCATGCGCCCTTGTTGCCAGGGGACGACGATTAAGGGCTGTGAGCCAGGCCCTTAATGTATCGCGTGCGCAGCT
ATCCGTTCGTGTTCATCGAAAACCGGGTTGGCAAGATGGTCGTCATCATCGCCAGCGTGATGATGCAGCCTTACTTGGCCGTATCATGGAGGCGATGGCA
GAGCTTCCCTCCTATGGCTATCGCCGGGTCTGGGCGCTGTTACGGCGTCAGTCAGAGGCAACACGACAGCCTGTGGTAAACGCCAAGCGGGTATATCGCG
TCATGCGCGATCATGGCCTGCTGCTGGAAAGAAAACCGGCGGCACCATTGGCCCAGCAGGCACATAAGGGGCGTGTTGCCGTCAAAGAGAGCAATCGACG
TTGGTGCTCGGATGGCTTTGAATTTCGCTGTGATAACGGTGAAAAACTACGGGTCACGTTCGCCATGGACTGCTGCGATAGGGAAGCGTTGGACTGGGCT
GCCAGTACCGGGGGTTACGACAGTGACACGGTGCAACATGTCATGCTGAGGTCTGTCGAACGTCGCTTCGGGGATTCGTTGCCGGCATCACCGGTGGAAT
GGCTGACAGATAATGGTTCGGCATATCGAGCCCATGAGACCCGTGCGTTTGCACGAGAAATCGGGTTGGAGCCCAGAACGACTGCGGTGAGAAGCCCACA
GAGCAACGGTATAGCGGAGAGCTTCGTGAAGACGATGAAGCGAGACTATATCGAGATGATGCCAAAACCGGATAGCCGAACAGCGGTAGGCAATTTGGCT
ATCGCGTTTGAGCATTATAACGAGCACCACCCGCACAGCGCTTTGGGATACCGTTCACCCCGGGAATATCTGCGCAGTCGGGTATCACAACCCTAAGCGG
GAAAAAGTGTCTGGACCTATGGGGTCAAATCCA
GTGTTAACAGGGGCTGAGCGCCGTCGACGCCGGACTCCGCAGGAAAAGATCTCCATCGTGCAGCAGACCTTTGAGCCCGGTATGACCGTCTCTCATGTCG
CTCGCTTGCACGACGTCAACGCCAACCAGCTTTTCAAATGGCGTAAGCAATTCCAGGAAGGGTCTCTCACGGCGATTTCCGCCGGTGAGGATGTTGTTCC
AGCCTCAGAGTTGGCTGCTGCCATCAAGCAGATCCGAGAGCTCCAGCGTTTGCTCGGGAAGAAGACCATGGAGAACGAGATCCTTAAAGAAGCCGTGGAG
TATGGTCGAGCAAAAAAATGGATAGCGCATGCGCCCTTGTTGCCAGGGGACGACGATTAAGGGCTGTGAGCCAGGCCCTTAATGTATCGCGTGCGCAGCT
ATCCGTTCGTGTTCATCGAAAACCGGGTTGGCAAGATGGTCGTCATCATCGCCAGCGTGATGATGCAGCCTTACTTGGCCGTATCATGGAGGCGATGGCA
GAGCTTCCCTCCTATGGCTATCGCCGGGTCTGGGCGCTGTTACGGCGTCAGTCAGAGGCAACACGACAGCCTGTGGTAAACGCCAAGCGGGTATATCGCG
TCATGCGCGATCATGGCCTGCTGCTGGAAAGAAAACCGGCGGCACCATTGGCCCAGCAGGCACATAAGGGGCGTGTTGCCGTCAAAGAGAGCAATCGACG
TTGGTGCTCGGATGGCTTTGAATTTCGCTGTGATAACGGTGAAAAACTACGGGTCACGTTCGCCATGGACTGCTGCGATAGGGAAGCGTTGGACTGGGCT
GCCAGTACCGGGGGTTACGACAGTGACACGGTGCAACATGTCATGCTGAGGTCTGTCGAACGTCGCTTCGGGGATTCGTTGCCGGCATCACCGGTGGAAT
GGCTGACAGATAATGGTTCGGCATATCGAGCCCATGAGACCCGTGCGTTTGCACGAGAAATCGGGTTGGAGCCCAGAACGACTGCGGTGAGAAGCCCACA
GAGCAACGGTATAGCGGAGAGCTTCGTGAAGACGATGAAGCGAGACTATATCGAGATGATGCCAAAACCGGATAGCCGAACAGCGGTAGGCAATTTGGCT
ATCGCGTTTGAGCATTATAACGAGCACCACCCGCACAGCGCTTTGGGATACCGTTCACCCCGGGAATATCTGCGCAGTCGGGTATCACAACCCTAAGCGG
GAAAAAGTGTCTGGACCTATGGGGTCAAATCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 369 bp | 122 aa | 92 | 460 | + | No |
Description : First part of the transposase
ORF sequence :
MVEVLTGAERRRRRTPQEKISIVQQTFEPGMTVSHVARLHDVNANQLFKWRKQFQEGSLTAISAGEDVVPASELAAAIKQIRELQRLLGKKTMENEILKE
AVEYGRAKKWIAHAPLLPGDDD
AVEYGRAKKWIAHAPLLPGDDD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 909 bp | 302 aa | 388 | 1296 | + | No |
Description : Second part of the transposase
ORF sequence :
RSRGVWSSKKMDSACALVARGRRLRAVSQALNVSRAQLSVRVHRKPGWQDGRHHRQRDDAALLGRIMEAMAELPSYGYRRVWALLRRQSEATRQPVVNAK
RVYRVMRDHGLLLERKPAAPLAQQAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCCDREALDWAASTGGYDSDTVQHVMLRSVERRFGDSLPA
SPVEWLTDNGSAYRAHETRAFAREIGLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNLAIAFEHYNEHHPHSALGYRSPREYLRSRVS
QP
RVYRVMRDHGLLLERKPAAPLAQQAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCCDREALDWAASTGGYDSDTVQHVMLRSVERRFGDSLPA
SPVEWLTDNGSAYRAHETRAFAREIGLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNLAIAFEHYNEHHPHSALGYRSPREYLRSRVS
QP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 401 aa | 92 | 1296 | + | Yes |
Chemistry : DDE
ORF sequence :
MVEVLTGAERRRRRTPQEKISIVQQTFEPGMTVSHVARLHDVNANQLFKWRKQFQEGSLTAISAGEDVVPASELAAAIKQIRELQRLLGKKTMENEILKE
AVEYGRAKKMDSACALVARGRRLRAVSQALNVSRAQLSVRVHRKPGWQDGRHHRQRDDAALLGRIMEAMAELPSYGYRRVWALLRRQSEATRQPVVNAKR
VYRVMRDHGLLLERKPAAPLAQQAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCCDREALDWAASTGGYDSDTVQHVMLRSVERRFGDSLPAS
PVEWLTDNGSAYRAHETRAFAREIGLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNLAIAFEHYNEHHPHSALGYRSPREYLRSRVSQ
P
AVEYGRAKKMDSACALVARGRRLRAVSQALNVSRAQLSVRVHRKPGWQDGRHHRQRDDAALLGRIMEAMAELPSYGYRRVWALLRRQSEATRQPVVNAKR
VYRVMRDHGLLLERKPAAPLAQQAHKGRVAVKESNRRWCSDGFEFRCDNGEKLRVTFAMDCCDREALDWAASTGGYDSDTVQHVMLRSVERRFGDSLPAS
PVEWLTDNGSAYRAHETRAFAREIGLEPRTTAVRSPQSNGIAESFVKTMKRDYIEMMPKPDSRTAVGNLAIAFEHYNEHHPHSALGYRSPREYLRSRVSQ
P
Blast result :
Comments
ISAs17 is 84% aa similar to IS2.
ORFAB has been generated in silico, assuming a translational frameshifting mechanism (as described
for IS2).
ORFAB has been generated in silico, assuming a translational frameshifting mechanism (as described
for IS2).
References
1] F. Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20