ISAs23

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1032 bp

Ends


IR Length : 23/28

IRL : GGCTCTGTCCCAATTGTGTGTTGAAAGGCTAAGCTTGAAGTAGGCCATCA
IRR : GGCTATGTCCCAATTACGTGTTGCATGGGGTGCCCCAGCGCCTCATGCAA

Insertion site


Left flankDirect repeatRight flankDR Length
ACCAGCAAGGCGATATAAAAGGGGGATCCCTC8

DNA sequence

GGCTCTGTCCCAATTGTGTGTTGAAAGGCTAAGCTTGAAGTAGGCCATCACCTTGAGGTCTACCATCATGGATACCCCAGCCTTTCAACACCTGTTATCC
CTCTTTCCACTGCTTACCCTGCGCCAGCGCCGTGTTGCCCAGCAGGAGCTCACGGCGCCACACGCCATTACCTTACTCAATACCCTGTTACCAGCATGCA
GCGGTTGCCCTCATTGTCAGGCCGAACCTGCTCAGCTGGCGCCTTGGGGATGGAGTCGGGGACTGCGCCGCTACCGTTGCAAACTGTGTCTGCGCACCAG
CACCGTGCTCACCAAGACGCCCCTGGCCCGGTTGCGTAAAGCCCAGTGCTGGGAAGACTACGCGCAAGCCCTCATTGAAGGGCTGACCGTCCGTCAGGCA
GCGCTGCGTTGCGGCGTCAGCAAAAATACCGCCTTCCTCTGGCGTCACCGCTTCTTGCGCGCGATGGCAACCCATCAAGCCACACGGGAAGAGGGCATCG
TTGAGGTTGATGAGACCTTCTTCCTCGAATCGTTCAAGGGGCAACGGGGACTGCCGCGTCCGCCACGGCATCGCGGTGGTAAAGGGCGAACCCGTGGAAC
CGGGCCGGATTACATTCCGGTGATGGTGGTGCAAGACCGGGCAGGCCACCATGCGGACTTTCAGCTGGATAAGATGAATGCCGAAACAGTGATAGCGGTC
TTGAAGCCCTTGGTTTCACCTGACGCGGTACTGTGTAGCGATGGTGCGGGCGTATATGCCAGTTTCAGCCAAGCGCAGGGGGTAACTCATCAGGTGGTGC
ACAATCGTCGGGGCGAACGGGTGGTTGGTGCGTATCACATCCAGCATGTAAACGGATATCACCATCGGTTGAAAGAGTGGATGGAGCGGTTCCATGGCGT
GGCGACCCACTACCTGAAGAATTACCTGGGTTGGCGCAGGATGCTGGAACGTTACGGAAGGGGGGTGAATATCAAAGTGTGTTTGCATGAGGCGCTGGGG
CACCCCATGCAACACGTAATTGGGACATAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
963 bp320 aa681030+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDTPAFQHLLSLFPLLTLRQRRVAQQELTAPHAITLLNTLLPACSGCPHCQAEPAQLAPWGWSRGLRRYRCKLCLRTSTVLTKTPLARLRKAQCWEDYAQ
ALIEGLTVRQAALRCGVSKNTAFLWRHRFLRAMATHQATREEGIVEVDETFFLESFKGQRGLPRPPRHRGGKGRTRGTGPDYIPVMVVQDRAGHHADFQL
DKMNAETVIAVLKPLVSPDAVLCSDGAGVYASFSQAQGVTHQVVHNRRGERVVGAYHIQHVNGYHHRLKEWMERFHGVATHYLKNYLGWRRMLERYGRGV
NIKVCLHEALGHPMQHVIGT

 

Blast result :
Comments
ISAs23 is 90% aa similar to ISAhy1.
References
1] Friedlhem Pfeiffer (2015) Direct submission.
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20