ISAs26

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1798 bp

Ends


Left end : CTTAAATGCGAAAGGGGACTCCCTCCGACGTAGGTGGGAGGGGAATTTCGCCGGGGCGTCAGCCCCGCTGGTTTTGAATGTATTGCTTGATCACCTCCAA II struct. : Yes
Right end : CCCGATGAAGCAGGAACCCGGTACTGCGCGAGCAGCTATCACCCAGTAATGGGAATCCCCCTCCTGTGACGCGAAGCGCTAAGGTGGGGGAGGATGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TTTGCGTCAAAGCCATCGCTCGCAGAATTCAA

DNA sequence

CTTAAATGCGAAAGGGGACTCCCTCCGACGTAGGTGGGAGGGGAATTTCGCCGGGGCGTCAGCCCCGCTGGTTTTGAATGTATTGCTTGATCACCTCCAA
CGGTGCTCCGCCGCAAGAGCCCGCAAAGTAAGAGCGTGACCAGAGTACGGGTTTGCTGTAAGCACCTCTCAAGTCCAGAAATTCATTACGCAGTCGCCTT
GATGTGACCGCCTTTAACGAGTTCACCAGTACCGATAGCTGCACCGTTGGCGCGTACTCTATCAGCATGTGAACATGATCAGCCTCTCCGTCACACTCTT
TCAGTTCCGCACCAAAATCTCTGCAAACCTCTGCCGCACACTGACCGAAGGAGGCGTGATGCAGATCCCCCAATATCGGCTTTCGATACTTGGTGATAAA
CACCAAATGTACGTGCAAAGCGTAGGCCGCATGGCGTGATTTATTAATTTTAGTGAATTGCATTCTGTCACTCAATCGGGCTTAATAGATATGGTAATAA
TGCTACACACTGAGCGCTAAATGCAAATTCTGAAAGCCTACAAATTCCGACTCGAACCGACCGAACAACAAGCGCAACGCCTGCGCCAGTTGGGCGGTTG
TGCTCGTTATGTCTGGAATCACGGGCTGGCAGAAACCAAACGCATTCTGGATGCTGGCGAAAAACTGCCTTCTGCCTTTGAGCTGAACAGGATGCTCACC
AGTTGGAAGAAAGACCCAGAAATGGCCTTTCTGGCCGAGGCATATACCGACAACCTCCAGCAGAAGCTCAAGGATTTGCATGGCGCGTGGATGCGCTGCT
TTGATAAGAAACTCGCCGCCAAGGCACCCGTATTCAAGAAAAAAAGCGACAACCGCGACGCTATCCGGTTCGTCAACTTCGACAAGTATTGCCGCCTTGA
AAAGGGTCGCGTGAAGCTGCCTGCCGGGCTGGCGTGGGTCAAGTTCCGTCAGTCCCAGCCAGTTGAAGGGAAAATTAAAAACGCGACCGTCAGCCAAGCA
GCCGGGCACTGGTATATCTCCTTTCAGGTCGAGCAGGAGACGCTTGAGCCAATTCACCCCTCCGGCTCAATGGTCGGGCTAGATGCGGGGATCGCCAAGC
TCGCAACCCTGTCAGACGGCACGGTGTTTGAGCCCGTCAACAGCTTCAAAACCAATCAGACCAAGCTGGCAAGGCTCCAACGCCAGTTGAGCAAAAAGGT
AAAGTTCAGCAGCAACTGGAAGAAACAAAAAGCCAAGATCCAGCGGCTGCACTCACATATCGCCAATACCCGCCGCGATTACCTTCACAAGGTCACAACG
ACTATCAGCAAAAACCACGCGATGATCGTCATTGAGGATTTGAAGGTTGCCAGCATGTCAAAATCAGCAGCGGGCACGACCAGCCAGCCGGGGCGCAACG
TCCGGGCAAAATCAGGGCTGAATCGTTCGATATTAGATCAGGGCTGGTTTGAGCTGCGCCGCCAGCTCGAATATAAGCAGGCATGGCGAGGCGGTCAGGT
GTTGGCGATAAACCCGGCCTACACCAGCCAGCAGTGCGCCTGTTGTGGGCATACTGCGAAGGATAACCGCCAGACGCAAAGCCAGTTTAAATGCGCTGCG
TGTGGTTATGAGGCGAACGCCGATGTGAACGGCGCTCGCAATATTTTAGCGGCGGGACACGCCGTTTTAGCCTGTGGAGGCGTGGTGCAATCAGGCCGCC
CGATGAAGCAGGAACCCGGTACTGCGCGAGCAGCTATCACCCAGTAATGGGAATCCCCCTCCTGTGACGCGAAGCGCTAAGGTGGGGGAGGATGTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
405 bp134 aa46359-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MQFTKINKSRHAAYALHVHLVFITKYRKPILGDLHHASFGQCAAEVCRDFGAELKECDGEADHVHMLIEYAPTVQLSVLVNSLKAVTSRRLRNEFLDLRG
AYSKPVLWSRSYFAGSCGGAPLEVIKQYIQNQRG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1227 bp409 aa5211747+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MQILKAYKFRLEPTEQQAQRLRQLGGCARYVWNHGLAETKRILDAGEKLPSAFELNRMLTSWKKDPEMAFLAEAYTDNLQQKLKDLHGAWMRCFDKKLAA
KAPVFKKKSDNRDAIRFVNFDKYCRLEKGRVKLPAGLAWVKFRQSQPVEGKIKNATVSQAAGHWYISFQVEQETLEPIHPSGSMVGLDAGIAKLATLSDG
TVFEPVNSFKTNQTKLARLQRQLSKKVKFSSNWKKQKAKIQRLHSHIANTRRDYLHKVTTTISKNHAMIVIEDLKVASMSKSAAGTTSQPGRNVRAKSGL
NRSILDQGWFELRRQLEYKQAWRGGQVLAINPAYTSQQCACCGHTAKDNRQTQSQFKCAACGYEANADVNGARNILAAGHAVLACGGVVQSGRPMKQEPG
TARAAITQ

 

Blast result :
Comments
ISAs26 is 90% aa (ORFA : the transposase) and 82% aa (ORFB) similar to ISEc41.
References
1] Friedlhem Pfeiffer (2015) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20