ISEal1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Escherichia albertii | Escherichia albertii CB9786 |
DNA section
IS Length : 1245 bp
Ends
IR Length : 30/39
IRL : TGATCCTACCCGCGAATAGTGGACACGGGACTAAGTGAGTAAACTCTTAA
IRR : TGATCTTACCCACGTAATGTGGACACAGTGCTAAGCGAGATTCTGGTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTGGTGAAG | TATAACATGC | 0 |
DNA sequence
TGATCCTACCCGCGAATAGTGGACACGGGACTAAGTGAGTAAACTCTTAATCCAGAGGTGACTCATGACAAAATCAGTATCAACCAGTAAGAAGCCCCGC
AAGCAACACACGCCTGAATTTCGCAACGAAGCACTGAAGCTTGCGGAACGTATAGGCGTCGCTGCTGCTGCCCGTGAACTCAGCCTGTACGAATCCCAGC
TCTATGCATGGCGCAGCAAGATTAACAATGCCCGCTGTTCTTCCGAACGTGAGCAGGAAATGTCCGTTGAAATTGCCCGTCTTAAGCGGCAACTGGCGGA
ACGGGACGAGGAACTGGCTATCCACCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTTATCGAAAAACATCGGGCTGAGTTCA
GCGTCAAAGCCATGTGCCGTGTACTTCGGGTTGCCCGCAGCGGCTGGTATGCCTGGCTCCTGCGTCGCCATCAAATAAGCCCGCGCCAGCAGTTCCGGCT
TACCTGTGATGAGGCTGTCCGTAACGCATTCGTTGAGGCAAAACAGCGCTATGGTGCGCCGCGTCTTGCTGATGAGTTGCCGGAGTACAACATTAAAACC
ATTGCTGCCAGTCTGCGTCGTCAGGGGTTGCGGGCGAAAGCGACCCGCAAGTTCAGTCCGGTCAGTTACCGTGAGCACGGCTTGCCCGTGTCAGAGAATC
TGCTGAAACAGGATTTTTACGCCAGCGGCCCAAACCAGAAGTGGGCAGGTGACATCACCTACTTACGTACGGATGAAGGCTGGCTGTATCTGGCGGTGGT
GATTGACCTGTGGTCGCGAGCCGTCATTGGCTGGTCGATGTCGCTGCGTATGACGGCACAACTTGCCTGCGATGCACTGCAAATGGCGCTCTGGCGGCGT
AAACATCCGGAAAATGTCATTGTTCACACCGACCGTGGTGGCCAGTACTGTTCAGAGGATTATCAGACCTTGCTGGAACGTCATAATCTGCGGGGGAGCA
TGAGTGCAAAGGGCTGCTGCTATGACAATGCCTGTGTGGAAAGCTTCTTTCACTCGTTGAAAGTGGAGTGTATCCACGGAGAACGCTTTGCCAGCCGGGA
AATAATGCGGACGATAGTGTTTAATTATATCGAGTGTGATTACAATCGCTGGCGTCGCCACAGCGCGTGTGGCGGTCTCAGCCCGGAACAATTTGAAAAC
CAGAATCTCGCTTAGCACTGTGTCCACATTACGTGGGTAAGATCA
AAGCAACACACGCCTGAATTTCGCAACGAAGCACTGAAGCTTGCGGAACGTATAGGCGTCGCTGCTGCTGCCCGTGAACTCAGCCTGTACGAATCCCAGC
TCTATGCATGGCGCAGCAAGATTAACAATGCCCGCTGTTCTTCCGAACGTGAGCAGGAAATGTCCGTTGAAATTGCCCGTCTTAAGCGGCAACTGGCGGA
ACGGGACGAGGAACTGGCTATCCACCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTTATCGAAAAACATCGGGCTGAGTTCA
GCGTCAAAGCCATGTGCCGTGTACTTCGGGTTGCCCGCAGCGGCTGGTATGCCTGGCTCCTGCGTCGCCATCAAATAAGCCCGCGCCAGCAGTTCCGGCT
TACCTGTGATGAGGCTGTCCGTAACGCATTCGTTGAGGCAAAACAGCGCTATGGTGCGCCGCGTCTTGCTGATGAGTTGCCGGAGTACAACATTAAAACC
ATTGCTGCCAGTCTGCGTCGTCAGGGGTTGCGGGCGAAAGCGACCCGCAAGTTCAGTCCGGTCAGTTACCGTGAGCACGGCTTGCCCGTGTCAGAGAATC
TGCTGAAACAGGATTTTTACGCCAGCGGCCCAAACCAGAAGTGGGCAGGTGACATCACCTACTTACGTACGGATGAAGGCTGGCTGTATCTGGCGGTGGT
GATTGACCTGTGGTCGCGAGCCGTCATTGGCTGGTCGATGTCGCTGCGTATGACGGCACAACTTGCCTGCGATGCACTGCAAATGGCGCTCTGGCGGCGT
AAACATCCGGAAAATGTCATTGTTCACACCGACCGTGGTGGCCAGTACTGTTCAGAGGATTATCAGACCTTGCTGGAACGTCATAATCTGCGGGGGAGCA
TGAGTGCAAAGGGCTGCTGCTATGACAATGCCTGTGTGGAAAGCTTCTTTCACTCGTTGAAAGTGGAGTGTATCCACGGAGAACGCTTTGCCAGCCGGGA
AATAATGCGGACGATAGTGTTTAATTATATCGAGTGTGATTACAATCGCTGGCGTCGCCACAGCGCGTGTGGCGGTCTCAGCCCGGAACAATTTGAAAAC
CAGAATCTCGCTTAGCACTGTGTCCACATTACGTGGGTAAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 65 | 364 | + | No |
Description : First part of the transposase
ORF sequence :
MTKSVSTSKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWRSKINNARCSSEREQEMSVEIARLKRQLAERDEELAIHQKAATYFAKRLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 286 | 1215 | + | No |
Description : Second part of the transposase
ORF sequence :
AATGGTGRGTGYPPKGRDILREAPEMKYVFIEKHRAEFSVKAMCRVLRVARSGWYAWLLRRHQISPRQQFRLTCDEAVRNAFVEAKQRYGAPRLADELPE
YNIKTIAASLRRQGLRAKATRKFSPVSYREHGLPVSENLLKQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSLRMTAQLACDALQM
ALWRRKHPENVIVHTDRGGQYCSEDYQTLLERHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGERFASREIMRTIVFNYIECDYNRWRRHSACGGLSP
EQFENQNLA
YNIKTIAASLRRQGLRAKATRKFSPVSYREHGLPVSENLLKQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSLRMTAQLACDALQM
ALWRRKHPENVIVHTDRGGQYCSEDYQTLLERHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGERFASREIMRTIVFNYIECDYNRWRRHSACGGLSP
EQFENQNLA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 65 | 1215 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKSVSTSKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWRSKINNARCSSEREQEMSVEIARLKRQLAERDEELAIHQKGRDILREAPEM
KYVFIEKHRAEFSVKAMCRVLRVARSGWYAWLLRRHQISPRQQFRLTCDEAVRNAFVEAKQRYGAPRLADELPEYNIKTIAASLRRQGLRAKATRKFSPV
SYREHGLPVSENLLKQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSLRMTAQLACDALQMALWRRKHPENVIVHTDRGGQYCSEDY
QTLLERHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGERFASREIMRTIVFNYIECDYNRWRRHSACGGLSPEQFENQNLA
KYVFIEKHRAEFSVKAMCRVLRVARSGWYAWLLRRHQISPRQQFRLTCDEAVRNAFVEAKQRYGAPRLADELPEYNIKTIAASLRRQGLRAKATRKFSPV
SYREHGLPVSENLLKQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSLRMTAQLACDALQMALWRRKHPENVIVHTDRGGQYCSEDY
QTLLERHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGERFASREIMRTIVFNYIECDYNRWRRHSACGGLSPEQFENQNLA
Blast result :
Comments
ISEal1 is 91% aa similar to ISEc16.
There is one copy in E. albertii strain CB9786.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
There is one copy in E. albertii strain CB9786.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Tadasuke Ooka, Tetsuya Hayashi (2015) Direct submission.