ISPsme1
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KJ920385 | Y | Pseudomonas mendocina | Pseudomonas mendocina LM7 |
DNA section
IS Length : 1064 bp
Ends
IR Length : 21/25
IRL : CTGAATTCATAGAATAAGTGCAACGTTTGAAACGATAGGCAGAGAGTCAG
IRR : CTGAATTCAACACATAAGTGCAACGCTCACCCCTGCATAAACTTCGTACG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCCAGTGTTT | GCTTC | AAATACTAAG | 5 |
DNA sequence
CTGAATTCATAGAATAAGTGCAACGTTTGAAACGATAGGCAGAGAGTCAGCCACCGGTAGAATCCAGGCTCTCACACCGAAGGATTCAAGCGCAGATGAC
TACGCATTACCGGCAGCTGACTCAGGGCCAACGTTACCAGATTGAGGCCGGCCTGAGCGCCGGAAAAAGCCAGGCAAGCATCGCGAAACAGGTTGGCGTA
CATCCCTCGACGATCAGTCGTGAGGTTCGCCGCAACAGCTCCCAGAAGACCTACAAAGCGGTTGCTGCGACCCATGAAAGTGATGCCCGTCGAGCGCAGG
CGCGCAAGCACTGCAAGCCATTAACGTGGTTCAGCCATTATCTGCCGCTCTGGCTAAAACACGGTATGAGCCCGGAGCAGATTGCTCAGCGCCTGAAGCA
AGAGCAGCCGGAGCGCGCGGTCAGTCACGAGTGGATTTATCGGTTTATCGCCACCGACAAACACGCAGGTGGCGAGCTATACAAGTATTTGCGGCACCGC
AGGAAGCTGTACCGAAAACGCTACGGAAGCCACGACCGGCGCGGACAGTTGCGTAATCGCGTATCGATAACCGAGCGCCCTGCTGAGGTCGAAGCCCGCG
AGCGTCTAGGTGATTGGGAGGGTGACACGGTGCATGGCGTAGGCGGCAATCTGGTCACGCTGGTTGATCGTAAAAGCGGCTACCTGAGCGCCTATCCGGT
CAAGCGCAGGACGCGCCGGCAGGTAACGCGGGCGATCAATCTGTTGCTCAAGGGGCATGCTGTTCACACGCTGACGCTGGATAACGGGAAGGAGTTTGCC
GGGCATGAACGCATTGCGCATCGGAGCCAGTGCCAAGTCTTTTTTGCAGAACCCTATTCATCCTGGCAGCGAGGCACTAATGAAAATACCAACGGCCTGC
TCCGGCAGTATTTTCCCAAGGGCGGCGACTTCAGCAAGCTGACCGTCGAGGCGGTCAGTCGCGTGGTAACGAAGATCAATTTACGCCCGCGAAAACGCCT
GGGCTGGAAGACGCCGTACGAAGTTTATGCAGGGGTGAGCGTTGCACTTATGTGTTGAATTCAG
TACGCATTACCGGCAGCTGACTCAGGGCCAACGTTACCAGATTGAGGCCGGCCTGAGCGCCGGAAAAAGCCAGGCAAGCATCGCGAAACAGGTTGGCGTA
CATCCCTCGACGATCAGTCGTGAGGTTCGCCGCAACAGCTCCCAGAAGACCTACAAAGCGGTTGCTGCGACCCATGAAAGTGATGCCCGTCGAGCGCAGG
CGCGCAAGCACTGCAAGCCATTAACGTGGTTCAGCCATTATCTGCCGCTCTGGCTAAAACACGGTATGAGCCCGGAGCAGATTGCTCAGCGCCTGAAGCA
AGAGCAGCCGGAGCGCGCGGTCAGTCACGAGTGGATTTATCGGTTTATCGCCACCGACAAACACGCAGGTGGCGAGCTATACAAGTATTTGCGGCACCGC
AGGAAGCTGTACCGAAAACGCTACGGAAGCCACGACCGGCGCGGACAGTTGCGTAATCGCGTATCGATAACCGAGCGCCCTGCTGAGGTCGAAGCCCGCG
AGCGTCTAGGTGATTGGGAGGGTGACACGGTGCATGGCGTAGGCGGCAATCTGGTCACGCTGGTTGATCGTAAAAGCGGCTACCTGAGCGCCTATCCGGT
CAAGCGCAGGACGCGCCGGCAGGTAACGCGGGCGATCAATCTGTTGCTCAAGGGGCATGCTGTTCACACGCTGACGCTGGATAACGGGAAGGAGTTTGCC
GGGCATGAACGCATTGCGCATCGGAGCCAGTGCCAAGTCTTTTTTGCAGAACCCTATTCATCCTGGCAGCGAGGCACTAATGAAAATACCAACGGCCTGC
TCCGGCAGTATTTTCCCAAGGGCGGCGACTTCAGCAAGCTGACCGTCGAGGCGGTCAGTCGCGTGGTAACGAAGATCAATTTACGCCCGCGAAAACGCCT
GGGCTGGAAGACGCCGTACGAAGTTTATGCAGGGGTGAGCGTTGCACTTATGTGTTGAATTCAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 96 | 1058 | + | No |
Chemistry : DDE
ORF sequence :
MTTHYRQLTQGQRYQIEAGLSAGKSQASIAKQVGVHPSTISREVRRNSSQKTYKAVAATHESDARRAQARKHCKPLTWFSHYLPLWLKHGMSPEQIAQRL
KQEQPERAVSHEWIYRFIATDKHAGGELYKYLRHRRKLYRKRYGSHDRRGQLRNRVSITERPAEVEARERLGDWEGDTVHGVGGNLVTLVDRKSGYLSAY
PVKRRTRRQVTRAINLLLKGHAVHTLTLDNGKEFAGHERIAHRSQCQVFFAEPYSSWQRGTNENTNGLLRQYFPKGGDFSKLTVEAVSRVVTKINLRPRK
RLGWKTPYEVYAGVSVALMC
KQEQPERAVSHEWIYRFIATDKHAGGELYKYLRHRRKLYRKRYGSHDRRGQLRNRVSITERPAEVEARERLGDWEGDTVHGVGGNLVTLVDRKSGYLSAY
PVKRRTRRQVTRAINLLLKGHAVHTLTLDNGKEFAGHERIAHRSQCQVFFAEPYSSWQRGTNENTNGLLRQYFPKGGDFSKLTVEAVSRVVTKINLRPRK
RLGWKTPYEVYAGVSVALMC
Blast result :
Comments
ISPsme1 is 94% aa similar to ISPpu17. ISPsme1 has been identified by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis ISPsme1 is present in 2 copies in the genome of Pseudomonas mendocina strain LM7. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM25, Pseudomonas sp. ZM2.
References
1] Szuplewska, M., and Bartosik, D. (2014) Direct submission.