ISPsp7
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KJ920384 | Y | Pseudomonas sp. | Pseudomonas stutzeri A1501 Pseudomonas sp. ZM2 |
DNA section
IS Length : 1113 bp
Ends
IR Length : 21/27
IRL : GGCGGCGGCAAAAACTGAGTGCAACACTTGACCTTTCCGGTACGGTGCTG
IRR : GGCGGTTGCAGGAGCTGAGCGCAACACGGTTATTGAATTGAAGCCGGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCTGTCAAT | AGAAG | TTTCGCCGAC | 5 |
| AACGCTATTA | GCG | CCATGATCAA | 3 |
| CCCGTTGGAT | GACCATAAGC | 0 |
DNA sequence
GGCGGCGGCAAAAACTGAGTGCAACACTTGACCTTTCCGGTACGGTGCTGCCCCTATGTCTTATACCGAACTCAGCGTTGAAGAGCGCGCCACCATTCAA
ATCGGTCGTACCCAAGGCTTCAGCCTGCGCAGGATTGCCTGCTTGATCAACCGATCCCCTTCGACCATCAGCCGTGAGCTGCGCCGTAACCGAGGTGCTT
GCGGTGGCTACTCGGCCCGTCTGGCCCAGCAGCAAATGCAGACCCGCCGCCAGGTTTGTCGACCGATGCGAAAACTGTTGCCGGGTAGCGAGCGCTTCGA
ACTGGTGACCCATATGCTGCGTGAGCGTTTGTCTCCCGAGCAGATTGCCGGCAAGCTGCGCAGCATGAACATACCCAACCTGCGAGATGCCTACGTCTGT
CGCGAGACGATCTACAACGCGATCTATGCCCTGCCAGTGGGTGAGCTGCGTAAGGAGCTGATCATCTGTCTGCGCCAAGGCAAGACGACGCGCCGGCCGC
GCTCTGGCGGCGTGGATCGGCGCGGCCAGATCCCTGAGATGGTCAGTATTCATGTGCGCCCGCCGGAGATTGAAGACAGGCTGATGCCGGGGCATTGGGA
AGGCGACCTCATCAAGGGTAAGGCCAACGCCTCGTCCGTCGGTACGCTGGTGGAACGCACCAGTGGCTACCTGATGCTGGTGAAGATGAACGACGCGACG
GCGACTTCGGCGCTGGAAGGCTTCAGCGCCGCGCTCAATGGCATGCCGCTGGAGATGCGCAAGAGCATGACTTACGACCAGGGCCGGGAGATGGCGCGAC
ACGCCGAGATCACCCAAAGAACCGGCGTGGCGATCTACTTCTGCGACCCGCACAGCCCCTGGCAGCGCGGCAGCAACGAAAACATCAACGGCCTGATTCG
CCAGTACTTGCCCAAGGGCACAGACCTGTCGGTACATAGCCAAGAGGAGCTGGACGCCATTGCGCTGCAACTGAACATGCGACCGCGCAAGCGCTTCGAC
TTCAAATGCCCCATCGAGGTGATGGGAGAGGTCATGCAAAATGCCATGGCGATGCGGCATGATGCTCCGGCTTCAATTCAATAACCGTGTTGCGCTCAGC
TCCTGCAACCGCC
ATCGGTCGTACCCAAGGCTTCAGCCTGCGCAGGATTGCCTGCTTGATCAACCGATCCCCTTCGACCATCAGCCGTGAGCTGCGCCGTAACCGAGGTGCTT
GCGGTGGCTACTCGGCCCGTCTGGCCCAGCAGCAAATGCAGACCCGCCGCCAGGTTTGTCGACCGATGCGAAAACTGTTGCCGGGTAGCGAGCGCTTCGA
ACTGGTGACCCATATGCTGCGTGAGCGTTTGTCTCCCGAGCAGATTGCCGGCAAGCTGCGCAGCATGAACATACCCAACCTGCGAGATGCCTACGTCTGT
CGCGAGACGATCTACAACGCGATCTATGCCCTGCCAGTGGGTGAGCTGCGTAAGGAGCTGATCATCTGTCTGCGCCAAGGCAAGACGACGCGCCGGCCGC
GCTCTGGCGGCGTGGATCGGCGCGGCCAGATCCCTGAGATGGTCAGTATTCATGTGCGCCCGCCGGAGATTGAAGACAGGCTGATGCCGGGGCATTGGGA
AGGCGACCTCATCAAGGGTAAGGCCAACGCCTCGTCCGTCGGTACGCTGGTGGAACGCACCAGTGGCTACCTGATGCTGGTGAAGATGAACGACGCGACG
GCGACTTCGGCGCTGGAAGGCTTCAGCGCCGCGCTCAATGGCATGCCGCTGGAGATGCGCAAGAGCATGACTTACGACCAGGGCCGGGAGATGGCGCGAC
ACGCCGAGATCACCCAAAGAACCGGCGTGGCGATCTACTTCTGCGACCCGCACAGCCCCTGGCAGCGCGGCAGCAACGAAAACATCAACGGCCTGATTCG
CCAGTACTTGCCCAAGGGCACAGACCTGTCGGTACATAGCCAAGAGGAGCTGGACGCCATTGCGCTGCAACTGAACATGCGACCGCGCAAGCGCTTCGAC
TTCAAATGCCCCATCGAGGTGATGGGAGAGGTCATGCAAAATGCCATGGCGATGCGGCATGATGCTCCGGCTTCAATTCAATAACCGTGTTGCGCTCAGC
TCCTGCAACCGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 56 | 1084 | + | No |
Chemistry : DDE
ORF sequence :
MSYTELSVEERATIQIGRTQGFSLRRIACLINRSPSTISRELRRNRGACGGYSARLAQQQMQTRRQVCRPMRKLLPGSERFELVTHMLRERLSPEQIAGK
LRSMNIPNLRDAYVCRETIYNAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIPEMVSIHVRPPEIEDRLMPGHWEGDLIKGKANASSVGTLVE
RTSGYLMLVKMNDATATSALEGFSAALNGMPLEMRKSMTYDQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELD
AIALQLNMRPRKRFDFKCPIEVMGEVMQNAMAMRHDAPASIQ
LRSMNIPNLRDAYVCRETIYNAIYALPVGELRKELIICLRQGKTTRRPRSGGVDRRGQIPEMVSIHVRPPEIEDRLMPGHWEGDLIKGKANASSVGTLVE
RTSGYLMLVKMNDATATSALEGFSAALNGMPLEMRKSMTYDQGREMARHAEITQRTGVAIYFCDPHSPWQRGSNENINGLIRQYLPKGTDLSVHSQEELD
AIALQLNMRPRKRFDFKCPIEVMGEVMQNAMAMRHDAPASIQ
Blast result :
Comments
ISPsp7 is 94% aa similar to ISUnCu4. ISPsp7 has been identified by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis ISPsp7 is present in 2 copies in the genome of Pseudomonas sp. ZM2. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas sp. LM7, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM13, Pseudomonas sp. LM14, Pseudomonas sp. LM25, Pseudomonas sp. ZM2.
References
1] Szuplewska, M., and Bartosik, D. (2014) Direct submission.