ISAs15

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1062 bp

Ends


IR Length : 19/20

IRL : GGCGTTGTTTCCCAAATCAGAGTGACCTGAATTGAACTTATGGTGGAGAC
IRR : GGCGTTGTTGCCCAAATCAGCCATTAGGCCACAACTTCCCCAGCCCCAGG

Insertion site


Left flankDirect repeatRight flankDR Length
ACAAAACCTTACGCTATCACCCTGAAATAAGAG9

DNA sequence

GGCGTTGTTTCCCAAATCAGAGTGACCTGAATTGAACTTATGGTGGAGACGCTGATCTGATCACCAAATCCCTCTTCAAATCAGCATCATGGGCAAGTCG
CTTCACGCCATATCCAACTGGTCACAGTACAACCGTTCCCTGGTCAAACGCGGCTCGCTAACGTTCTGGGTTGATGAGAATGTCGTTCATAACTGGTTCC
ATCAAGAACATCATGGCCGACGTGGACGAAGCGCCCTCTACACCGACCAGACCATCTGTACCTTTCTCATGCTCAAAGGCATTTTCAACCTCTCCCTGCG
GGCGACACAGGGCCTGCTCGACTCACTGTTCGAGCTGATGAATGTGCCGTTGTGTGCCCCCGACTACAGCTGTGTCAGCAAGCGCGCCAGTACTGTGAAG
GTGGCATACCGCCAGCCACCCAAGGGCAAGGTTACCGATTTGGTTATCGATTCCACCGGCTTGAAAGTGTTTGGCGAGGGCGAATGGAAGGTCAGAAAGC
ATGGCTATGACAAGCGCCGGGTCTGGCGCAAATTGCATCTGGCGGTGGACCCGGCGACCCACGACATCGTGGCAGCCGAGGCCTCGTTGGAAAATGTTCA
TGATGCCGAGGTGTTGCCGACCCTGTTCAATCCGCTACGCCGAGCACTTGGGACCGTGTATGCCGATGGCGCATATGACACCAAGGCCTGCCATGAACTG
ATAAAGCGCAAAGGAGCCAAGGCCTGTATTCCGCCGCGCAAGAATGCCGGTTTATGGGAAAAGGGGCATCCACGCAATGAAGCTGTTCTGGTGATGCGCA
AGGAGGGGCTGACTCACTGGAAGCAGCAGTCCGGGTATCATCGGCGCTCATTGGCCGAGACGGCGATGTATAGGTTCAAACAATTGATGACGGGAAAAAT
CAGTCTACGTAACTACAACGGACAAGTGGGAGAGATCATGGCCTACGTGTGTGCGATGAACAAGCTGAATGCCCTTGGTCTGCCGGTTAGAGAGCCCCAA
GTGTAGCTATCACCTGGGGCTGGGGAAGTTGTGGCCTAATGGCTGATTTGGGCAACAACGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
918 bp305 aa891006+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGKSLHAISNWSQYNRSLVKRGSLTFWVDENVVHNWFHQEHHGRRGRSALYTDQTICTFLMLKGIFNLSLRATQGLLDSLFELMNVPLCAPDYSCVSKRA
STVKVAYRQPPKGKVTDLVIDSTGLKVFGEGEWKVRKHGYDKRRVWRKLHLAVDPATHDIVAAEASLENVHDAEVLPTLFNPLRRALGTVYADGAYDTKA
CHELIKRKGAKACIPPRKNAGLWEKGHPRNEAVLVMRKEGLTHWKQQSGYHRRSLAETAMYRFKQLMTGKISLRNYNGQVGEIMAYVCAMNKLNALGLPV
REPQV

 

Blast result :
Comments
ISAs15 is 91% aa similar to ISAs4.
References
1] Pfeiffer, F. (2014) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20