ISAs14

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas salmonicida
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1062 bp

Ends


IR Length : 17

IRL : GGCGTTGTTTCCCAAATAGATGCAGCTTGAATGGAACTAACTGAGGACTC
IRR : GGCGTTGTTTCCCAAATCAACCATCAAGCCACAACTGCCCCAGCCCCAGG

Insertion site


Left flankDirect repeatRight flankDR Length
GCATTGATATGGGGTAACAGCACATCCAGCTCA9

DNA sequence

GGCGTTGTTTCCCAAATAGATGCAGCTTGAATGGAACTAACTGAGGACTCGGTGATCTGATCACCACCATCCTCTTCAAATCTGCATCATGGGCACGTCG
CTTCACCCCATCACCAACTGGCCTCAATACAACAAATCATTGATTAACCGTGGTTCACTCACCTTCTGGGTTGATGCCGAGGCCATGAACAACTGGTTTC
ACCACGGCCATCATGGGTGCCGGGGCCGCAGCCAACTCTACACTGACCAGACCATCTGCACCTTCCTGATGCTCAAGGGGATTTTCAACCTCACTCTGCG
GGCCACTCAGGGGCTACTCGACTCCCTGTTCGAGTTGATGAATGTGCCGCTGTGCGCACCGGACTATAGCTGTGTAAGTAAGCGAGCCCGCACGGTGAAA
GTGGCCTATCGACAGCCCCCCAAGGGACGTATCACCGACCTGGTTATCGACTCGACCGGCCTGAAGGTATTTGGTGAAGGCGAGTGGAAGGTCAGAAAAC
ACGGCACCGAGAAGCGGCGAGTGTGGCGCAAGTTGCATCTGGCGGTAGACCCTGTGACCCACGATATCGTGGCGGCGGAAGTCTCACTGGAAAATGTCCA
TGATGCCGAGGTGTTGCCCACCTTGCTCAACCCTCTGCGGCGTAAGCTGGGACATGTTTATGCGGATGGAGCCTATGACAGCAAAGCCAGCCATCAGCTT
ATCTCGTGCAAAGGGGCAACAGCCTGTATCCCGCCTCGCAAGAACGCTGGACTCTGGAAAAAGGGACATCCAAGAAATGAGGCTGTGCTGGTAATGCGCA
AGGAAGGGCTGGCGCACTGGAAGAAGATATCGGGGTATCACCGTCGCTCACTGGCCGAGACGGCAATGTATCGGTTCAAGCAGTTGCTGGCGGGAAAAAT
CAGTCTGCGTAACTACAACGGCCAAGTGGGAGAAGTCATGGCGTATGTCTGCGCCATAAACAAGCTGAATACACTGGGCATGCCTGTCAGAAAGCCCCGA
GTGTAACGGTCACCTGGGGCTGGGGCAGTTGTGGCTTGATGGTTGATTTGGGAAACAACGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
918 bp305 aa891006+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGTSLHPITNWPQYNKSLINRGSLTFWVDAEAMNNWFHHGHHGCRGRSQLYTDQTICTFLMLKGIFNLTLRATQGLLDSLFELMNVPLCAPDYSCVSKRA
RTVKVAYRQPPKGRITDLVIDSTGLKVFGEGEWKVRKHGTEKRRVWRKLHLAVDPVTHDIVAAEVSLENVHDAEVLPTLLNPLRRKLGHVYADGAYDSKA
SHQLISCKGATACIPPRKNAGLWKKGHPRNEAVLVMRKEGLAHWKKISGYHRRSLAETAMYRFKQLLAGKISLRNYNGQVGEVMAYVCAINKLNTLGMPV
RKPRV

 

Blast result :
Comments
ISAs14 is 96% aa similar to ISAs4, and 97% to ISAs13.
In the genome, this transposon has been targetted by another transposon.
References
1] Pfeiffer, F. (2014) Direct submission

2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20