ISAs13

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP022426 ND Aeromonas salmonicida
Aeromonas punctata
Aeromonas salmonicida
Aeromonas caviae
Aeromonas salmonicida subsp. pectinolytica 34mel
DNA section
IS Length : 1062 bp

Ends


IR Length : 17/20

IRL : GGCGTTGTTGCCCAAATCGATGCAGCTTGAATGGAACTAACTGAGGACTT
IRR : GGCGCTGTTTCCCAAATCAACCATCAAGCCACAACTGCCCCAGCCCCAAG

Insertion site


Left flankDirect repeatRight flankDR Length
AACGCGGTTTAACCATAACAACAGACCTTTTCA9
CTGGTTTTGACCGATATTTCACGGTCATCTCAC9
GCTTCTTGATCTGGTGTAAATCCATCCAAAGCA9
GAATTTCTTTGAGTTTTCTCACAGTGATAGGCC9

DNA sequence

GGCGTTGTTGCCCAAATCGATGCAGCTTGAATGGAACTAACTGAGGACTTGGTGATCTGATCACCACCATCTCCTTCAAGTCTGCATCATGAGCACGTCG
CTTCACCCCATCACCAACTGGCCTACATACAACAAATCATTGATTAACCGTGGTTCACTCACCTTCTGGGTTGATGCCGAGGCCATGAACAACTGGTTTC
ACCACGGCCATCATGGGCGGCGGGGCCGCAGCCAACTATACACTGACCAGACCATCTGCACCTTCCTGATGCTCAAGGGGATTTTCAACCTCACTCTGCG
GGCCACCCAGGGACTACTCGACTCCCTGTTCGAGTTGATGAACGTACCGCTTTGTGCCCCCGATTACAGCTGTGTCAGCAAGCGAGCACGCACGGTGGCG
GTGGCTTATCGACAGCCCTCTAAGGGGCGCATCACTGACTTGGTTATCGATTCGACGGGCCTGAAGGTATTTGGTGAAGGCGAGTGGAAGGTCAGAAAAC
ACGGCGCCGAGAAGCGGCGAGTGTGGCGCAAGTTGCATCTGGCGGTAGACCCGGTGACCCACGATATCGTGGCGGCGGAAGTCTCGCTGGAAAATGTCCA
TGATGCCGAGGTGTTGCCGACCTTGCTCAACCCACTGCGGCGCAAGCTGGGACGTGTTTATGCGGATGGCGCCTATGACAGCAAAGCCAGCCATCAGCTA
ATCTCGCGCAAAGGGGCGACAGCCTGTATCCCGCCTCGCAAGAACGCTGGGCTATGGGAAAAAGGACATCCAAGAAATGAGGCTGTGCTGGTGATGCGCA
AGGAAGGGCTGGCGCACTGGAAGAAGATATCGGGGTATCACCGTCGCTCGCTGGCGGAAACGGCGATGTATCGGTTCAAGCAGTTGCTGGCGGGGAAAAT
CAGTCTGCGTAACTACAATGGCCAAGTGGGAGAAGTCATGGCGTATGTCAGTGCCATAAACAAACTGAATACCCTTGGTCTGCCTGTCAGAAAGCCCCGA
GTGTAACGGTCACTTGGGGCTGGGGCAGTTGTGGCTTGATGGTTGATTTGGGAAACAGCGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
918 bp305 aa891006+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSTSLHPITNWPTYNKSLINRGSLTFWVDAEAMNNWFHHGHHGRRGRSQLYTDQTICTFLMLKGIFNLTLRATQGLLDSLFELMNVPLCAPDYSCVSKRA
RTVAVAYRQPSKGRITDLVIDSTGLKVFGEGEWKVRKHGAEKRRVWRKLHLAVDPVTHDIVAAEVSLENVHDAEVLPTLLNPLRRKLGRVYADGAYDSKA
SHQLISRKGATACIPPRKNAGLWEKGHPRNEAVLVMRKEGLAHWKKISGYHRRSLAETAMYRFKQLLAGKISLRNYNGQVGEVMAYVSAINKLNTLGLPV
RKPRV

 

Blast result :
Comments
ISAs13 is 97% aa similar to ISas4.
References
1] Pfeiffer, F. (2014) Direct submission
2] F. Pfeiffer, M.A. Zamora-Lagos, M. Blettinger, A. Yeroslaviz, A. Dahl, S. Gruber, B.H. Habermann (2018) BMC Genomics 19 (1), 20