ISAeme1

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1256 bp

Ends


IR Length : 15/18

IRL : CAGGGCAAGATCGCGAACGTTTTTTAAGCAGGGCATGAGGATTTGACCGA
IRR : CAGGGCGAGAGCGTGAACATATTGTTAAATTGATGCCAGTTCAGCTAACC

Insertion site


Left flankDirect repeatRight flankDR Length
CCTCAATTATAGTCTGTGCGGCCCACTGACTTTG10
AAAAAACAGTCAGGGTTTACGCTAATCGAATTGA10
ACGCTTGCCTTAGCTGGAAACCCTGCTGCTTATC10
AATAATGGCCAGCGATGACCAGCTGGTCCAGGCG10
TATAACAACTGGTTCAAGTCGTTCGCTTCGCCAGGGCAAGATCG20

DNA sequence

CAGGGCAAGATCGCGAACGTTTTTTAAGCAGGGCATGAGGATTTGACCGACCGAACAGGGTGTGATCTTATACTCTCTTTTACGGGAGTTTCGTCATGAA
CCATCAAGCGGCATTTACCCAATTCTTCTCCGAACTTGAAGACCCTCGTCAGACAACCAAAATCGCGTACCCCTTCTTCGATGTGGTTTTTCTCACCGTC
TGTGCTGCTATTGGTGGGGCTCAAGGTTGGGAAGATATCGAGCTTTTCGGCGAGGCACATCTAGAATGGTTTCAGCGTAATGGATTGTTTCATAACGGAT
TACCTGTTCACGACACCATAGCCAGGATCATCTCCCGTATTCAACCGGAGCAATTCCAGAACGCCTTTGTCCGCTGGATGCAAGCAACATCAGAACGTAC
CGATGGTGAGCTGATTGCCATTGACGGCAAGACCTTACGCAGCTCTTACGACCGAGACAGTCGTCAGTCTACCATTCATATGGTCAGTGCCTTTGCCGCT
GAGAATCGTCTGGTGCTCGGGCAAGTCAAAACAGATGCGAAATCTAACGAAATCACCGCCATTCCTGAATTACTGGCACTCCTCGATATAAAAGGCTGCC
TGGTATCCATCGATGCCATGGGCTGCCAGACTGAGATAGCCTCAACCATAATCAAGGGCGGTGGCGACTACTTGCTGGCGGTAAAAGGTAACCAGCCCAC
ACTGCATAATGCAGTACGCGCAGCTCTGGGCGAGTCGACCAAGAAGCCACTGAGTGAAGAAACGTTCAGTGTCGAGAAAGAGCACGGTCGCATTGATGGC
CGCGAGTACCATGTGTTACCAGCCGGAGCACTATCAGAGCAATTTCCAGAGTGGAAAGCGCTGAAGAGTGTTGGTGTCGCAATATCCTATCGAATTGAAA
ATATGAAAAAATTTTCGATGGAGTATCGCTACTACATCAGCTCGGCTGAACTGACACCAGAGCAGTTTGCCTCAGCGGTACGAGGGCACTGGGCAATTGA
AAATAGCCTGCATTGGGTTCTGGATGTCGTGATGAATGAGGATGCCTGCCAGATATATCGAGGTGATGCTCCTCAAATATTGGCCTGTGCACGACACATG
GCGCAAAACATGCTGCGAGCCGAAACCAGCCGTAAGGCAAGCTTGCGGAAGAAGCAGCAATTTGCGGGCATGAGCAGCCCATATCTGGAAAAGGTACTGG
ATGCTGGGTTAGCTGAACTGGCATCAATTTAACAATATGTTCACGCTCTCGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1137 bp378 aa961232+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MNHQAAFTQFFSELEDPRQTTKIAYPFFDVVFLTVCAAIGGAQGWEDIELFGEAHLEWFQRNGLFHNGLPVHDTIARIISRIQPEQFQNAFVRWMQATSE
RTDGELIAIDGKTLRSSYDRDSRQSTIHMVSAFAAENRLVLGQVKTDAKSNEITAIPELLALLDIKGCLVSIDAMGCQTEIASTIIKGGGDYLLAVKGNQ
PTLHNAVRAALGESTKKPLSEETFSVEKEHGRIDGREYHVLPAGALSEQFPEWKALKSVGVAISYRIENMKKFSMEYRYYISSAELTPEQFASAVRGHWA
IENSLHWVLDVVMNEDACQIYRGDAPQILACARHMAQNMLRAETSRKASLRKKQQFAGMSSPYLEKVLDAGLAELASI

 

Blast result :
Comments
ISAeme1 is 81% aa similar to ISAs12.
References
1] Chai, B., Wang, H., and Chen, X. (2012) J. Bacteriol. 194, 6693-6694