ISAs7
- Family IS3
- Group IS51
Isoform ISAs5Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000644 | ND | Aeromonas salmonicida | Aeromonas salmonicida subsp. salmonicida A449 Aeromonas salmonicida subsp. salmonicida A449 plasmid 5 |
DNA section
IS Length : 1232 bp
Ends
IR Length : 22/26
IRL : TGAATCGCCCCCAGTTCCCTAGACACCTCACAGCCTTAAAATGAGGCAAC
IRR : TGAATCGCCCCTGGTTTTCTAGACACTCTGTTGCTGACAAATAAACTGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCGACTTCA | ATA | TGCCTGATAT | 3 |
| CACAAAAGCA | AAC | AGACCGAGCG | 3 |
| CGCCGAAGGT | CTCGGGCCTG | 0 | |
| CCATGATGCG | ACC | ATTCGCCATA | 3 |
| CTTGCGCTTC | CTG | ATGCGGATCC | 3 |
| CCAACCCGAT | ACC | ATTCTCGGTC | 3 |
| GATTCGCAAG | GTG | TTCAAGAAAC | 3 |
| CATTCCAACG | GAC | ATTTATGCTT | 3 |
| TCAATAAGAC | CACAATGGTG | 0 | |
| TTGATGGATT | CGCTGATCCT | 0 | |
| TAGTGGAGTA | CTAGATTCCG | 0 | |
| TCGGATCAAC | GTATTTGATT | 0 | |
| CAATGAGCTG | GTCAAAAGAA | 0 |
DNA sequence
TGAATCGCCCCCAGTTCCCTAGACACCTCACAGCCTTAAAATGAGGCAACTATTGGAGGTGTTATGACCCGTCAGCGTTATCCAGAAACATTCAAAATCG
AAGCGGTTAAACAAATTACCGAGCGTGGCCGGCCGGTGGCTGACGTAGCACGAGCCCTCGGGGTATCCAGCCACAGCCTGTATGCATGGCTCAAGCAGTA
CGGTAAGCCCGCTGATCAGCAGCACGAAGAGTCAGCCTTACAAGCCGAGATCCGCCGTCTGAAAGCCGAACTGCGCCAAGTCACTGAGGAGCGCAACATT
TTAAAGGAGGCCGCCGCGTACTTTGCCAGCGAGTCAAGGAAAGGTACGCGTTCATAACCTCCCGACTCGACCGGTACAGTGTCGTTACCCTGTGCCAGAC
CCTGGGGGTTCACCGAAGTGGCTTCTACGCGTGGCTGAAACAGCCCGCATCGTTACGCCAGCAAGCGGATGAACGGCTGACCGGATTGGTCAAGCAATCC
TGGCTGGAAAGTGGTGCTGTCTACGGTTATCGCAAGATCCATCGCGATCTATGTGACTTGGGGGAAGCCTGCGGCAAGCACCGTGTGGCCAAATTAATGA
GGCGGGAGGGGCTGCGTTCGCAAACTGGCTATCAACGCCGTAAGGGGCATTATGGAGGCAAGAGCCCACAGGCTGCCCCAAACACGCTGGCACGTCAATT
TAAGGTGCCAGCCCCCAACATCTCCTGGGTGACTGACATTACCTACATCCGAACTCAAGAAGGGTGGTTGTACCTGGCGGTGGTGCTCGATCTGTTTTCT
CGCCAGATCATCGGCTGGGCGATGAAGCCACGCATGACGGCAGGCTTGGCGGTTGATGCGTTATTGATGGCGGTTTGGCGACGTAAGCCAACGCAGCCGG
TACTGGTGCATTCGGATCAGGGCAGCCAGTTTACCGGTGGTGAATGGCAGGACTTTCTGAAGGCCCACAACCTGAGTTGCAGTATGAGCCGACGTGGTAA
CTGCCACGACAATGCTGTCGCCGAGAGCTTCTTTCAGTTGCTCAAACGGGAGCGGATCAAGCGCAGGATTTACTCGACAAGAGACGAAGCCCGCAGCGAC
GTATTTGATTATATCGAAATGTTTTACAACCCAATCAGGCGCCATGGTTCCAATGATGGGCTGTCGCCGGTAGAGTTTGAAAGGCAGTTTATTTGTCAGC
AACAGAGTGTCTAGAAAACCAGGGGCGATTCA
AAGCGGTTAAACAAATTACCGAGCGTGGCCGGCCGGTGGCTGACGTAGCACGAGCCCTCGGGGTATCCAGCCACAGCCTGTATGCATGGCTCAAGCAGTA
CGGTAAGCCCGCTGATCAGCAGCACGAAGAGTCAGCCTTACAAGCCGAGATCCGCCGTCTGAAAGCCGAACTGCGCCAAGTCACTGAGGAGCGCAACATT
TTAAAGGAGGCCGCCGCGTACTTTGCCAGCGAGTCAAGGAAAGGTACGCGTTCATAACCTCCCGACTCGACCGGTACAGTGTCGTTACCCTGTGCCAGAC
CCTGGGGGTTCACCGAAGTGGCTTCTACGCGTGGCTGAAACAGCCCGCATCGTTACGCCAGCAAGCGGATGAACGGCTGACCGGATTGGTCAAGCAATCC
TGGCTGGAAAGTGGTGCTGTCTACGGTTATCGCAAGATCCATCGCGATCTATGTGACTTGGGGGAAGCCTGCGGCAAGCACCGTGTGGCCAAATTAATGA
GGCGGGAGGGGCTGCGTTCGCAAACTGGCTATCAACGCCGTAAGGGGCATTATGGAGGCAAGAGCCCACAGGCTGCCCCAAACACGCTGGCACGTCAATT
TAAGGTGCCAGCCCCCAACATCTCCTGGGTGACTGACATTACCTACATCCGAACTCAAGAAGGGTGGTTGTACCTGGCGGTGGTGCTCGATCTGTTTTCT
CGCCAGATCATCGGCTGGGCGATGAAGCCACGCATGACGGCAGGCTTGGCGGTTGATGCGTTATTGATGGCGGTTTGGCGACGTAAGCCAACGCAGCCGG
TACTGGTGCATTCGGATCAGGGCAGCCAGTTTACCGGTGGTGAATGGCAGGACTTTCTGAAGGCCCACAACCTGAGTTGCAGTATGAGCCGACGTGGTAA
CTGCCACGACAATGCTGTCGCCGAGAGCTTCTTTCAGTTGCTCAAACGGGAGCGGATCAAGCGCAGGATTTACTCGACAAGAGACGAAGCCCGCAGCGAC
GTATTTGATTATATCGAAATGTTTTACAACCCAATCAGGCGCCATGGTTCCAATGATGGGCTGTCGCCGGTAGAGTTTGAAAGGCAGTTTATTTGTCAGC
AACAGAGTGTCTAGAAAACCAGGGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 64 | 357 | + | No |
Description : First part of the transposase
ORF sequence :
MTRQRYPETFKIEAVKQITERGRPVADVARALGVSSHSLYAWLKQYGKPADQQHEESALQAEIRRLKAELRQVTEERNILKEAAAYFASESRKGTRS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 288 | 1214 | + | No |
Description : Second part of the transposase
ORF sequence :
GAQHFKGGRRVLCQRVKERYAFITSRLDRYSVVTLCQTLGVHRSGFYAWLKQPASLRQQADERLTGLVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRV
AKLMRREGLRSQTGYQRRKGHYGGKSPQAAPNTLARQFKVPAPNISWVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRK
PTQPVLVHSDQGSQFTGGEWQDFLKAHNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQ
FICQQQSV
AKLMRREGLRSQTGYQRRKGHYGGKSPQAAPNTLARQFKVPAPNISWVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRK
PTQPVLVHSDQGSQFTGGEWQDFLKAHNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQ
FICQQQSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 384 aa | 64 | 1214 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRQRYPETFKIEAVKQITERGRPVADVARALGVSSHSLYAWLKQYGKPADQQHEESALQAEIRRLKAELRQVTEERNILKGGRRVLCQRVKERYAFITS
RLDRYSVVTLCQTLGVHRSGFYAWLKQPASLRQQADERLTGLVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGK
SPQAAPNTLARQFKVPAPNISWVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLK
AHNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQSV
RLDRYSVVTLCQTLGVHRSGFYAWLKQPASLRQQADERLTGLVKQSWLESGAVYGYRKIHRDLCDLGEACGKHRVAKLMRREGLRSQTGYQRRKGHYGGK
SPQAAPNTLARQFKVPAPNISWVTDITYIRTQEGWLYLAVVLDLFSRQIIGWAMKPRMTAGLAVDALLMAVWRRKPTQPVLVHSDQGSQFTGGEWQDFLK
AHNLSCSMSRRGNCHDNAVAESFFQLLKRERIKRRIYSTRDEARSDVFDYIEMFYNPIRRHGSNDGLSPVEFERQFICQQQSV
Blast result :
Comments
ISAs7 is an isoform of ISAs5.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAs7 is 82% (orfAB, the transposase) aa similar to ISPen2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAs7 is 82% (orfAB, the transposase) aa similar to ISPen2.
References
1] Reith,M.E., Singh,R.K., Curtis,B., Boyd,J., Bouevitch,A., Kimball,J., Munholland,J., Murphy,C., Sarty,D., Williams,J., Nash,J., Johnson,S. and Brown,L. (2007) Direct submission GenBank