ISfinder Mirror Site

ISHbo7

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001690	ND	Halogeometricum borinquense	Halogeometricum borinquense DSM 11551

DNA section

IS Length : 1432 bp

Ends

Left end : GGAAACACAAGGAGAAAGCCCCGCCCTTTAGGGCGGGGATGAATCCGACAGTCGATGACACAAACCACCGACGATAGCACGGCCTGATATTCCAACAGAT II struct. : Yes
Right end : TCCACGCGAAAGCGTACTTGAGAACCGAGGAAACGCGCAACCTGAATATCCCCTTCGGGGAATCCTCGTCGCCAGACTCCGTCTGGCGGGCAACCAGAAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CCGTCTGTCGTA	TTAC	GCAAGGCGTTCTG	gaac

DNA sequence

GGAAACACAAGGAGAAAGCCCCGCCCTTTAGGGCGGGGATGAATCCGACAGTCGATGACACAAACCACCGACGATAGCACGGCCTGATATTCCAACAGAT
ACTTAGGATTCCGCACCTAATTACATTTGTAATGGTCACGGTGACTGTCACCGCGAAGTTCCACAACCCATCCCTCTCACGGCGCAAAGAGTGGCAACAC
GCCGCTCGCCTCTACCGTGACACCAAACAGTTCTGTATCGACGGATGGGAAAACGACGACTTCGACAAGTCAGTGACCACGGCCAGCATCGACAACGCCC
TCTACTCGGCTATCCAGAACCAAGCCATCCGAGAGGCGAAATCCGACCACAACAAGGACGGAGAGGTTCGCTACCGAGAGAGTCAACCATTCGCCGTCAA
CAATCAGAACTGGGAACTCGACACGACCGAGAATGGCACGGTCGTCGTCGGCTTCCCCTGCGTCTCCCAATGGTGGTACACCCCGATAGAGGTGTACGAC
GATATTGCCGACCCCGTAGACCGACTGGTCGAGGGTGATGCCGACAAGACCCGCCTACAGGTCTACCGTCGCGGAGACGACTGGTACTGTACGTTCAATA
TCGAGTACGACGCTGACACGTCGGGTGAGACACCCATCGGTGTCGATATTGGTGAACGACACATCCTCGCGGTGACGGCCTACGGCGAAGACGAGTCGAT
GCTGGTGTCGGGTGGTGAGGCGAAGTATGTTCGACGCAAATATCGTTCCCTACGCGATTCGCTTTCGGAAGCGGGTGCGCTTCGCGCACGCAACCGTGTG
GGTGACAAAGAACAGCGTCGAATCAAGGACTTGAACCACAAACTCTCCCGTCGCCTTATCACGTTCGCGGAACAGTTCGAGAATCCCGTCATTCGGATGG
AAGACCTCGAAGGCATCCGCGAGAACAGTTCGTGGTCGGGAGTTCACTCGTGGCACTTCCACCAACTCCAACAGTTCATCACGTACAAAGCCGAACGCGC
TGGTATCCGCGTCGAGGCGGTCGATGCGTACCATACCAGCCAGCGGTGTTCGGCCTGTGGGTCGATGGGCAATCGTGATGGCGACCACTTTTCGTGTTCG
GAGTGTGGTCGAGGACGCCACGCTGACCTGAACGCGTCGGAGAACATTGCACAACGGGAGGGTGAACCATGCACGGGCTAACGCTTCGGGCGAGTCGAAC
CGTGCTACCTCGCTGGTTGAATAGCCAGTCGTCATTGACGCCCGCTGTATGCGGGGAGGAAGGCCCCATTGACAGGGCTACACGCGCTGGAAAGCACGTC
CTTGGTCACAACTGGACGGCTACCGCTGACGTTCCACGCGAAAGCGTACTTGAGAACCGAGGAAACGCGCAACCTGAATATCCCCTTCGGGGAATCCTCG
TCGCCAGACTCCGTCTGGCGGGCAACCAGAAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1047 bp	349 aa	132	1178	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MVTVTVTAKFHNPSLSRRKEWQHAARLYRDTKQFCIDGWENDDFDKSVTTASIDNALYSAIQNQAIREAKSDHNKDGEVRYRESQPFAVNNQNWELDTTE
NGTVVVGFPCVSQWWYTPIEVYDDIADPVDRLVEGDADKTRLQVYRRGDDWYCTFNIEYDADTSGETPIGVDIGERHILAVTAYGEDESMLVSGGEAKYV
RRKYRSLRDSLSEAGALRARNRVGDKEQRRIKDLNHKLSRRLITFAEQFENPVIRMEDLEGIRENSSWSGVHSWHFHQLQQFITYKAERAGIRVEAVDAY
HTSQRCSACGSMGNRDGDHFSCSECGRGRHADLNASENIAQREGEPCTG

Blast result :

Comments

ISHbo7 is 95% aa similar to ISHli4 orfB.

References

1] Malfatti,S., Tindall,B.J., Schneider,S., Fahnrich,R., Lapidus,A., Labuttii,K., Copeland,A., Glavina Del Rio,T., Nolan,M., Chen,F., Lucas,S., Tice,H., Cheng,J.F., Bruce,D., Goodwin,L., Pitluck,S., Anderson,I., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., D'haeseleer,P., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Chain,P. (2009) Stand Genomic Sci 1 (2), 150-159.