ISAs6
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000644 | ND | Aeromonas salmonicida | Aeromonas salmonicida subsp. salmonicida A449 |
DNA section
IS Length : 0 bp
Ends
IR Length : 21/26
IRL : TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTAGAGTCGCCAC
IRR : TGTAGTGGTCAACTAAATCCGGACACTTGATTAGGCCGATCCTCGGCCTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCACCTGAAC | CTCGCACCGA | 0 | |
| TCGCTCAGGG | CGGTGTGTGG | 0 | |
| CCCGGCTGTT | ATTGTCGCCG | 0 |
DNA sequence
TGTAGTGGTCTAATCATCCCGGACACCATTTTAGGTGGTAGAGTCGCCACCATAATCTGAGGTGTTCAATGACAAGATTGCGTCGCTCATTTACTGCCGA
GTTCAAACTGGAAGCCGCATCCCTGGTGGTCGACCAGGGGTATTCAGTCCCCGAAGCCAGCCGTTCGCTGGATGTCGGTGAAACCGTACTTCGCCGCTGG
GTTCAGCAGCTTCAATCCGAACGCTGTGGCACTACCCCAATCAGCAAAGCGCTGACTCCGGAGCAGCAGAAAATCCAGGAGCTGGAAGCCCGTATCAACC
GGCTCGAGCGCGAAAAGGACATACTAAAAAAGGCTACTGCTCTCTTGATGGCGGACGAGTTCACACGTACGCGCTGATAGACCGATTAAGGGAGCAAGCC
CCCATCACCTTGGTCTGCTGCGCTTTTGATGTTCCCAAGTCCTGTTTTTACGACTATTTAGCCCGCAAGCGGACGATTAACCGTGAGCGGATGAAACAAC
GAAGCGAGTTGCGCCGTTTGTTCAAAGAGAGCCGGGATTCCGCAGGCAGCCGCGCCTTGATGTCGATGATGCGAGAGCTGGGGTATCAGATTGGCCGATT
CAAAGTCCGTAACCTGATGAAGGAAGCTGGGCTGGCGTCCAAACAGCCGGGCGCACACCGTTATAAGGTTGCATGCTCTGAACGACCGGATATTCCGAAT
CTGCTGGCCCGCGAATTTGATGTTCCGCAACCCAATCAGGTGTGGTGTGGCGATATCACCTACGTCTGGACAAGTGCTCGTTGGCATTACTTAGCAGTGG
TGCTTGACCTTCATACCCGGCGAGTCGTGGGCTGGGCGATGTCTGACAAGCCTGATGCAGAGCTGGCAATCAAAGCACTGGAGATGGCCTATCAGCAGCG
GGGTTGCCCATCCGGTGTGCTGTTTCACTCCGACCAGGGCAGTCAATATGGCAGCCGGGCATTTCGGCAACGGTTGTGGCGCTATCGCATGACCCAGAGT
ATGAGCCGGCGTGGAAACTGCTGGGATAACGCCCCGATGGAGCGGTTGTTCAGAAGCCTGAAGTCAGAGTGGCTTCCTGCGACGGGATACGTGAGCCTGC
GGGAAGCGAAGCGGGATATCAGTTATTACCTGATGGATTACTACAACTGGCGGCGTCCACATCAACACAATGACGGGATACCGCCAGCAGAGGCCGAGGA
TCGGCCTAATCAAGTGTCCGGATTTAGTTGACCACTACA
GTTCAAACTGGAAGCCGCATCCCTGGTGGTCGACCAGGGGTATTCAGTCCCCGAAGCCAGCCGTTCGCTGGATGTCGGTGAAACCGTACTTCGCCGCTGG
GTTCAGCAGCTTCAATCCGAACGCTGTGGCACTACCCCAATCAGCAAAGCGCTGACTCCGGAGCAGCAGAAAATCCAGGAGCTGGAAGCCCGTATCAACC
GGCTCGAGCGCGAAAAGGACATACTAAAAAAGGCTACTGCTCTCTTGATGGCGGACGAGTTCACACGTACGCGCTGATAGACCGATTAAGGGAGCAAGCC
CCCATCACCTTGGTCTGCTGCGCTTTTGATGTTCCCAAGTCCTGTTTTTACGACTATTTAGCCCGCAAGCGGACGATTAACCGTGAGCGGATGAAACAAC
GAAGCGAGTTGCGCCGTTTGTTCAAAGAGAGCCGGGATTCCGCAGGCAGCCGCGCCTTGATGTCGATGATGCGAGAGCTGGGGTATCAGATTGGCCGATT
CAAAGTCCGTAACCTGATGAAGGAAGCTGGGCTGGCGTCCAAACAGCCGGGCGCACACCGTTATAAGGTTGCATGCTCTGAACGACCGGATATTCCGAAT
CTGCTGGCCCGCGAATTTGATGTTCCGCAACCCAATCAGGTGTGGTGTGGCGATATCACCTACGTCTGGACAAGTGCTCGTTGGCATTACTTAGCAGTGG
TGCTTGACCTTCATACCCGGCGAGTCGTGGGCTGGGCGATGTCTGACAAGCCTGATGCAGAGCTGGCAATCAAAGCACTGGAGATGGCCTATCAGCAGCG
GGGTTGCCCATCCGGTGTGCTGTTTCACTCCGACCAGGGCAGTCAATATGGCAGCCGGGCATTTCGGCAACGGTTGTGGCGCTATCGCATGACCCAGAGT
ATGAGCCGGCGTGGAAACTGCTGGGATAACGCCCCGATGGAGCGGTTGTTCAGAAGCCTGAAGTCAGAGTGGCTTCCTGCGACGGGATACGTGAGCCTGC
GGGAAGCGAAGCGGGATATCAGTTATTACCTGATGGATTACTACAACTGGCGGCGTCCACATCAACACAATGACGGGATACCGCCAGCAGAGGCCGAGGA
TCGGCCTAATCAAGTGTCCGGATTTAGTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 69 | 377 | + | No |
Description : First part of the transposase
ORF sequence :
MTRLRRSFTAEFKLEAASLVVDQGYSVPEASRSLDVGETVLRRWVQQLQSERCGTTPISKALTPEQQKIQELEARINRLEREKDILKKATALLMADEFTR
TR
TR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1050 bp | 349 aa | 182 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
NRTSPLGSAASIRTLWHYPNQQSADSGAAENPGAGSPYQPARARKGHTKKGYCSLDGGRVHTYALIDRLREQAPITLVCCAFDVPKSCFYDYLARKRTIN
RERMKQRSELRRLFKESRDSAGSRALMSMMRELGYQIGRFKVRNLMKEAGLASKQPGAHRYKVACSERPDIPNLLAREFDVPQPNQVWCGDITYVWTSAR
WHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFRQRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPA
TGYVSLREAKRDISYYLMDYYNWRRPHQHNDGIPPAEAEDRPNQVSGFS
RERMKQRSELRRLFKESRDSAGSRALMSMMRELGYQIGRFKVRNLMKEAGLASKQPGAHRYKVACSERPDIPNLLAREFDVPQPNQVWCGDITYVWTSAR
WHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFRQRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPA
TGYVSLREAKRDISYYLMDYYNWRRPHQHNDGIPPAEAEDRPNQVSGFS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1163 bp | 387 aa | 69 | 1231 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRLRRSFTAEFKLEAASLVVDQGYSVPEASRSLDVGETVLRRWVQQLQSERCGTTPISKALTPEQQKIQELEARINRLEREKDILKKGYCSLDGGRVHT
YALIDRLREQAPITLVCCAFDVPKSCFYDYLARKRTINRERMKQRSELRRLFKESRDSAGSRALMSMMRELGYQIGRFKVRNLMKEAGLASKQPGAHRYK
VACSERPDIPNLLAREFDVPQPNQVWCGDITYVWTSARWHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFR
QRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPATGYVSLREAKRDISYYLMDYYNWRRPHQHNDGIPPAEAEDRPNQVSGFS
YALIDRLREQAPITLVCCAFDVPKSCFYDYLARKRTINRERMKQRSELRRLFKESRDSAGSRALMSMMRELGYQIGRFKVRNLMKEAGLASKQPGAHRYK
VACSERPDIPNLLAREFDVPQPNQVWCGDITYVWTSARWHYLAVVLDLHTRRVVGWAMSDKPDAELAIKALEMAYQQRGCPSGVLFHSDQGSQYGSRAFR
QRLWRYRMTQSMSRRGNCWDNAPMERLFRSLKSEWLPATGYVSLREAKRDISYYLMDYYNWRRPHQHNDGIPPAEAEDRPNQVSGFS
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISAs6 is 81% aa similar to IS222.
ISAs6 is 81% aa similar to IS222.
References
1] Reith,M.E., Singh,R.K., Curtis,B., Boyd,J., Bouevitch,A., Kimball,J., Munholland,J., Murphy,C., Sarty,D., Williams,J., Nash,J., Johnson,S. and Brown,L. (2007) Direct submission to GenBank