ISAba30

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii D4
DNA section
IS Length : 1769 bp

Ends


Left end : CCCTGTGCGCGGCAAGCCCCGCCGTTCAGGGCGGGGAAGGATAGCGCGGACGGCGTAGCCGTCTTTGGGTTTAGTGGGGTGTTTGCTGCTGTTCGATGTA II struct. : Yes
Right end : TCGCCAGCAGCAGGAACCCACCGAAGCGACTCAGACCAGGCTCATTGCCGCGTCTGAGCGCCGTAGGAATCTCCGGCCTTCAGGCCGGGGAGGATGTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CTAACTGACGGAATTCTTTTCAA

DNA sequence

CCCTGTGCGCGGCAAGCCCCGCCGTTCAGGGCGGGGAAGGATAGCGCGGACGGCGTAGCCGTCTTTGGGTTTAGTGGGGTGTTTGCTGCTGTTCGATGTA
CTGGCGCACAATGGAAATCGGCGCGCCACCACAACTTGAGGCGAAGTAGGACGGCGACCACAGAACGCCCTTCCAGTAGCGTTTCTGGATGTCGGGCCGC
TCCTTGCGCAGCAAACGGCTGGACACGCCTTTGAGGCTGTTCACAAGGTTGGAGACGGCAACCTTGGGCGGGTACTCCACCAACAGGTGAACATGATCGT
CCTCGCCGTCCATCTCAATTAGCTGCGCCTCGAAGTCGGTGCAAACCTTGGCGAAGATCGTTCTCAGCCGGTTGATGGCGTCGCCGTCGAACACTCTGCG
GCGATATTTCGCCACAAAGACCAAATGGACGTGCATCTTGAAAACGCAGTGCCGTCCAAGTCGAATATCGTTGTCATCACTCATAGACCAAAAGTATGAT
TGAGGTATGAAACGACTTCAAGCCTTCAAGTACGAAATGGTACCAAACGGCCAGCAGGAACGGCAGATGCGCCGCTTCGCTGGCTCGTGCCGGTTCGTCT
ACAACAAGGCACTGGCGTTGCAGAAGGAGCGCTACGAGCGCGGTGAGAAGAAGCTCGGCTATGCCGGGCTGTGCAAGCTACTCACCGAGTGGCGCAATAG
CTCGGAAATGGCATGGTTGGCTGATGCGCCGGTTCACCCGCTGCAACAGACGCTCAAGGACTTGGAGCGGGCGTATTCCAACTTCTTTGCCAAGCGCGCC
GACTTCCCGCGCTTCAAAAAGAAGGGGTGCCACAGTAGCTTTCGCTATCCCGATCCGAAACAAATCAAGCTGGAGCAGCACAACAGCCGCATCTTTCTGC
CCAAGCTTGGCTGGATTCGCTACCGCAAGAGCCGCGATGTGCTGGGGGCGGTGAAGAACGTCACTGTCAGCCTCTCGGGCGGCAAGTGGTTCGTGTCCAT
TCAAACCGAACGAGAGGTCGAGCAGCCCATCCCGCAGGGTGGCGCGGTCGGTATCGACATGGGCATTGCCCGGTTCGCTACGATCTCGGACGGCAAGTTC
TACGCTCCTCTAAACAGCTTCAAGCGACACGAAAAGCGCTTGCGCTGCGCGCAGCAAGCGCTCTCCCGCAAGGTGAAATTCAGCAACAACTGGCAGAAGG
CGAAAGCCCGTATCCAGCGCATCCATTCCCGCATCGGCAACGCCCGCCGCGACTTCCTGCACAAGACCTCGACCGCGATCAGCCAAAACCACGCGATGGT
GTGTATCGAGGACTTGCAGGTACGGAACATGTCCAAGTCGGCGGCGGGTACTCCCGAACAGCCGGGAAGAAACGTTCGGGCCAAGTCCGGCCTGAACAAA
TCCATCCTCGATCAGGGCTGGTTCGAGTTCCGTCGCCAACTGGATTATAAACTGGCGTGGAATGGTGGGTGGCTCGTTGCCGTGCCGCCTAAGAACACCA
GCCGAACCTGCCCGTGTTGTGCTCATGTGTCGGCGGATAATCGCCAGACACAAGAGCGGTTCGAGTGCATAGAGTGCGGGTTCGAGGGTAATGCCGATGT
GGTCGGCGCGATCAATGTACTAAGGGCGGGGCACGCCCGGTTAGCCTGTGAAGTGAGCGATGCAGTAGGGTCGCCAGCAGCAGGAACCCACCGAAGCGAC
TCAGACCAGGCTCATTGCCGCGTCTGAGCGCCGTAGGAATCTCCGGCCTTCAGGCCGGGGAGGATGTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
414 bp137 aa48471-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MSDDNDIRLGRHCVFKMHVHLVFVAKYRRRVFDGDAINRLRTIFAKVCTDFEAQLIEMDGEDDHVHLLVEYPPKVAVSNLVNSLKGVSSRLLRKERPDIQ
KRYWKGVLWSPSYFASSCGGAPISIVRQYIEQQQTPH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1221 bp406 aa5071727+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRLQAFKYEMVPNGQQERQMRRFAGSCRFVYNKALALQKERYERGEKKLGYAGLCKLLTEWRNSSEMAWLADAPVHPLQQTLKDLERAYSNFFAKRADF
PRFKKKGCHSSFRYPDPKQIKLEQHNSRIFLPKLGWIRYRKSRDVLGAVKNVTVSLSGGKWFVSIQTEREVEQPIPQGGAVGIDMGIARFATISDGKFYA
PLNSFKRHEKRLRCAQQALSRKVKFSNNWQKAKARIQRIHSRIGNARRDFLHKTSTAISQNHAMVCIEDLQVRNMSKSAAGTPEQPGRNVRAKSGLNKSI
LDQGWFEFRRQLDYKLAWNGGWLVAVPPKNTSRTCPCCAHVSADNRQTQERFECIECGFEGNADVVGAINVLRAGHARLACEVSDAVGSPAAGTHRSDSD
QAHCRV

 

Blast result :
Comments
ISAba30 is 87% (orfA) and 75% (orfB) aa similar to IS609.
References
1] Hamidian, M., Hall, R.M (2014) Direct submission .