ISAba27
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP009534 | ND | Acinetobacter baumannii | Acinetobacter baumannii plasmid pMMA2 Acinetobacter baumannii AB030 Acinetobacter baumannii AbH12O-A2 |
DNA section
IS Length : 883 bp
Ends
IR Length : 17
IRL : AGACCTCTTGCGAAAGTAAAAACCACCTCAATCTAAATTTCATGAGATAT
IRR : AGACCTCTTGCGAAAGTCGTTATTGCAAGTTCATCCGATTTACCAGTGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTATCAAGAC | ATT | GATAATGCCG | 3 |
| TTAAATAATT | ATT | GATAAAATAT | 3 |
| ATGAACGTCT | ATT | AAATAATCCT | 3 |
| ATTAGAAATT | ATT | GAACAGCAGC | 3 |
DNA sequence
AGACCTCTTGCGAAAGTAAAAACCACCTCAATCTAAATTTCATGAGATATCAGAAATTAAATCGTTTTTCAGATTCTGAATTCCAGCGCTTGGTTGGTGT
ACCTCGACCAGTTTTTAGTGAAATGATCGAAGTTTTAAAAGAAGTAGAATCACTTAAAAAGAAATCTGGGCGTCCTCATACTTTAGCTATAGAGGATCAA
TTATTATTAACACTCAATTACTTACGGAATTACAGCACTCAATTGGAGTTGGCTGCAAATTACCATATCGCTGAAAGTAATGTGAATCGAACCATTAAAA
AGGTTGAAGATGCATTGATGAAATCAAGACGGTTTACTCTGCCAAAACGAAGCATTACCACAGCAGACGAACAGTTTAACTGGGTCATTATTGATGCGAC
AGAATGTTCAATAGAACGTCCGAAAAAAAAATCAGAGTAAGTTCTACAGTGGTAAAAAGAAGAAACATACGCTAAAAGCACAAGTGATCTATCATCCGAA
CAGCAAACAAATCATAGGGCTAGATATATCGAATGGCAGTCAGCATGACATTAAATTGGCAAGAAGAACGGTTAAAAAATTCAAACATTGTAAATATGTT
ATGACCGATTTAGGGTACTACGGATTAGAGCAAGATGGCTTTAAATTATTGATGCCAATAAAGAAAAAGAAGAATTTACCCTTATTTGATGTTGAGAAAA
AGTACAATAAAATGATTGGAAAAATACGAGTTGTGATCGAACATATTAATAGTCAATTGAAAACATTTAGAATATTAAGTGAACGCTATCGAAATAGACG
GAAAAGATTCGGTTTACGCATTAACTTAATCGCTGCACTGGTAAATCGGATGAACTTGCAATAACGACTTTCGCAAGAGGTCT
ACCTCGACCAGTTTTTAGTGAAATGATCGAAGTTTTAAAAGAAGTAGAATCACTTAAAAAGAAATCTGGGCGTCCTCATACTTTAGCTATAGAGGATCAA
TTATTATTAACACTCAATTACTTACGGAATTACAGCACTCAATTGGAGTTGGCTGCAAATTACCATATCGCTGAAAGTAATGTGAATCGAACCATTAAAA
AGGTTGAAGATGCATTGATGAAATCAAGACGGTTTACTCTGCCAAAACGAAGCATTACCACAGCAGACGAACAGTTTAACTGGGTCATTATTGATGCGAC
AGAATGTTCAATAGAACGTCCGAAAAAAAAATCAGAGTAAGTTCTACAGTGGTAAAAAGAAGAAACATACGCTAAAAGCACAAGTGATCTATCATCCGAA
CAGCAAACAAATCATAGGGCTAGATATATCGAATGGCAGTCAGCATGACATTAAATTGGCAAGAAGAACGGTTAAAAAATTCAAACATTGTAAATATGTT
ATGACCGATTTAGGGTACTACGGATTAGAGCAAGATGGCTTTAAATTATTGATGCCAATAAAGAAAAAGAAGAATTTACCCTTATTTGATGTTGAGAAAA
AGTACAATAAAATGATTGGAAAAATACGAGTTGTGATCGAACATATTAATAGTCAATTGAAAACATTTAGAATATTAAGTGAACGCTATCGAAATAGACG
GAAAAGATTCGGTTTACGCATTAACTTAATCGCTGCACTGGTAAATCGGATGAACTTGCAATAACGACTTTCGCAAGAGGTCT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 399 bp | 132 aa | 42 | 440 | + | No |
Description : First part of the transposase
ORF sequence :
MRYQKLNRFSDSEFQRLVGVPRPVFSEMIEVLKEVESLKKKSGRPHTLAIEDQLLLTLNYLRNYSTQLELAANYHIAESNVNRTIKKVEDALMKSRRFTL
PKRSITTADEQFNWVIIDATECSIERPKKKSE
PKRSITTADEQFNWVIIDATECSIERPKKKSE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 450 bp | 149 aa | 415 | 864 | + | No |
Description : Second part of the transposase
ORF sequence :
NVRKKNQSKFYSGKKKKHTLKAQVIYHPNSKQIIGLDISNGSQHDIKLARRTVKKFKHCKYVMTDLGYYGLEQDGFKLLMPIKKKKNLPLFDVEKKYNKM
IGKIRVVIEHINSQLKTFRILSERYRNRRKRFGLRINLIAALVNRMNLQ
IGKIRVVIEHINSQLKTFRILSERYRNRRKRFGLRINLIAALVNRMNLQ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 823 bp | 273 aa | 42 | 864 | + | Yes |
Chemistry : DDE
ORF sequence :
MRYQKLNRFSDSEFQRLVGVPRPVFSEMIEVLKEVESLKKKSGRPHTLAIEDQLLLTLNYLRNYSTQLELAANYHIAESNVNRTIKKVEDALMKSRRFTL
PKRSITTADEQFNWVIIDATECSIERPKKNQSKFYSGKKKKHTLKAQVIYHPNSKQIIGLDISNGSQHDIKLARRTVKKFKHCKYVMTDLGYYGLEQDGF
KLLMPIKKKKNLPLFDVEKKYNKMIGKIRVVIEHINSQLKTFRILSERYRNRRKRFGLRINLIAALVNRMNLQ
PKRSITTADEQFNWVIIDATECSIERPKKNQSKFYSGKKKKHTLKAQVIYHPNSKQIIGLDISNGSQHDIKLARRTVKKFKHCKYVMTDLGYYGLEQDGF
KLLMPIKKKKNLPLFDVEKKYNKMIGKIRVVIEHINSQLKTFRILSERYRNRRKRFGLRINLIAALVNRMNLQ
Blast result :
Comments
ISAba27 is 71% aa similar to ISNemu1B.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
ISAba27 disrupted blaOXA-23 in A. baumannii AbH12O-A2.
There are 4 copies in Acinetobacter baumannii strain AbH12O-A2, and 76 copies in Acinetobacter baumannii strain AB030.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
ISAba27 disrupted blaOXA-23 in A. baumannii AbH12O-A2.
There are 4 copies in Acinetobacter baumannii strain AbH12O-A2, and 76 copies in Acinetobacter baumannii strain AB030.
References
1] Zander E. (2014) Direct submission