ISAba28
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter baumannii | Acinetobacter baumannii |
DNA section
IS Length : 2740 bp
Ends
IR Length : 22
IRL : GTAAGCGTCCATTTTAGGACGTCCTATCCCAACTAAAGATCGCGCGATAA
IRR : GTAAGCGTCCATTTTAGGACGTATTGACGCCTTGCAGTTTTTGGCCAACC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAAGCGCTCT | CCCGCAAGG | TGAAATTCAG | 9 |
DNA sequence
GTAAGCGTCCATTTTAGGACGTCCTATCCCAACTAAAGATCGCGCGATAAGCTGTGCACTCATCATAACAGGAGAGTAACATGGCAAAGCGAGCTTACCG
CAACGCTGCACAATGGCAGCAATTAATCGACTTATGGCACACATCAGAGCTGAGCATCACTGAATTTTGCCAAACCCATCAACTCAGTACCATGTCATTT
TATAAATGGCGGCAACGCTTGGCTTCACAAACTGAGCAAGCGCCTGTTCGCCAGGACACACAGTTTATCGATTTATCCGCGTTGGCACACCAAGGCAACA
CCCGCTGGCATATTGTGTTGTCACTGGGCAATGGCGTTGAACTGACACTTAGCCAGCAGTAATGTTTATCCCTGCTGCGAACCAAAAAATCTGGCTGTGC
ACCACACCCGTGGATATGCGTAAATCCTTTAACGGTTTAAGTGCCCTGGTGCGCAATAAACTTTGCCATAATCCACAAAGTGGCCAGTACTTTGTATTTA
TCAATGCCCGCAAAACTCAGATGAAGGTGTTGTACTTCGAAAGCTCTGGCTATTGTGTCTGGGCTAAGCGCCTCGAGCAAGGCCAGTTTCCGGTGAAACC
GCATCCGAGTGGGATCCAATCTCTTACCGGCTGCACCCTGCAAATGATCGTTGACGGAATTACCGTACTTCGCCACAAACAAGCGAAGCGCTATGGGCAG
TCAAGCGCTTGATAAGTCTGTATAATTGAGGCTATGAAAATCAAGCCTTCATCCTCATCCAACGCGGCAACAGACGCCGAGACACTGGTTAATTTGACCA
GTGAAATCGCGCGTCTGCAGAGTTTGTTGCAACAGGCTTCTGCAGAGAATCAGGCTTGGTCAGAAAAGTACGGCAAGCTGGAAACCGAGCATACGTCATT
GCGCGAAACCTTCGCCGGGATTCAGCAGCGCTTAGCCTGGTTTGAGAAGCAGCTGTTTGGTCAGAAGTCAGAAAAGCGCGCCCTGGAATTGGGTATGCAG
TTAAGCTTACTGGGTGATATGGTGCCAGCGCTGACCCAGCCTGAAGGCGAAACCGAATATACCACCTACACCCGCAGAAAAGGCAAACAGCGCCCGGATG
ATTGCGTTAATGACAGTGGCCTGCGATTTAACGATAACGTACCGGTGAAAGTGATTACCTTGATACCCGATGAGCTAAAAGGGGAAGAGGCCGACCAGTA
TGAAGTGATTGGCGTAAAAAGCACCTTCCGCTTAGCGCAGCGCCCAGCGAGCTTCGAAGTGCTACGTTATGACCGGCAAATCATCAAACGCAAAGGCAGC
GACACTCTCCTGCCAAGTCAGGCGCCCTTTAATGTATTGGATAAAAGCGTGGCGGATGTGAGCTTTATCGTGGGGATGCTGGTCGATAAATTCCAATACC
ACTTGCCGCTGTATCGCCAGCACCAGCGTTTAGTTGCAGCGGGCATTACTTTAAGCCGCAGCACACTGGGCACCATCGTGGCGCGCGGTATTGATTTACT
CTATCCGATTGTGGATGCGATGCTAAGTAGCATCTTACAAAGCCAGGTGCTGGCAATGGACGAAACGCCGATTAAAGCAGGCAAAGCCGGGCCAGGTAAA
ATGAAGCAAAGTTACTTCTGGCCGGTGTATGGCGATAAGGATGAAATTGTTTTTACCTTCTCCACCAGCCGCGGGCGGCAACATATAGAAGAGATAGTAA
AGCATCGCTTTAAAGGCACCTTATTAAGCGATGGCTACAGCGCCTATGCCAGTTATATCAAAGCCAATGAAGGCTTAACCCATGCCCAGTGCTGGGTGCA
CAGTCGCCGGCAATTTATTGAAGCGGAAAACAGCTGGCCAGCGCCGGCCAAAGAAGCGATTAACCTAATCGCTAAGCTGTATGAGATAGAAGAGACTATC
CGAAACCAAAAGCTCACGGATGACAAAAAACGCCAATACCGCCTTACACACAGCAAACCGGTGGTTGACCGCTTCTTTACGTGGTGTGATGATACCCTGC
ATAACCTGACGCTGCTGCCCAAAGACCCCCTCTCCAAAGCGATTGGCTATGTACAAAGCAAAGAAATGGCACTGCGCGTCTTCCTGGAAGACCCTGATGT
ACCGCTGGATACAAACCATCTGGAGCGGGCGCTTCGGCCCATACCCATGGGGAGAAAAAATTGGTTGTTCTGTTGGACAGAAATCGGTGCAGAGCATGTT
GGCGTTATCCAAAGCCTGATAGTGACCTGCCGCTTACATAATATCAATGTAAACGATTATCTGACCGATGTGCTGCTGCGCATCAGCCAACACCCTGCCA
GCCTGGTGCACGAACTGACGCCACGGTATTGGAAAACTAAGTTCGCTGATAATCCGCTGCGCTCTGACCTCTTTGCTTTACCCACTACTTAGGTAGAATA
GCCTCCAGACGGAGGCGATATGCGCATAGAAGACCTAACCTTAGACGAATTACACGACCTGAACGAGCTTATCTGCAAGCGGATAGGGTACTTGCGTTTG
CAAAACGATATCACTGTTCTAAGCCAGCTACGTTTAGGCCAAAAAGTGCACTTCAAGGCCAAAGAAGGCCAAGTGTTCGGGGTGGTCATCAAAATCAACA
AGAAATCAGTGATGGTGGTCAGCGACGATAATCGCCAGTGGAAAATCCCACCAGGGCTGGTGCAGATCATGAAAGACGTCTAGTCAGGATGGTTGGCCAA
AAACTGCAAGGCGTCAATACGTCCTAAAATGGACGCTTAC
CAACGCTGCACAATGGCAGCAATTAATCGACTTATGGCACACATCAGAGCTGAGCATCACTGAATTTTGCCAAACCCATCAACTCAGTACCATGTCATTT
TATAAATGGCGGCAACGCTTGGCTTCACAAACTGAGCAAGCGCCTGTTCGCCAGGACACACAGTTTATCGATTTATCCGCGTTGGCACACCAAGGCAACA
CCCGCTGGCATATTGTGTTGTCACTGGGCAATGGCGTTGAACTGACACTTAGCCAGCAGTAATGTTTATCCCTGCTGCGAACCAAAAAATCTGGCTGTGC
ACCACACCCGTGGATATGCGTAAATCCTTTAACGGTTTAAGTGCCCTGGTGCGCAATAAACTTTGCCATAATCCACAAAGTGGCCAGTACTTTGTATTTA
TCAATGCCCGCAAAACTCAGATGAAGGTGTTGTACTTCGAAAGCTCTGGCTATTGTGTCTGGGCTAAGCGCCTCGAGCAAGGCCAGTTTCCGGTGAAACC
GCATCCGAGTGGGATCCAATCTCTTACCGGCTGCACCCTGCAAATGATCGTTGACGGAATTACCGTACTTCGCCACAAACAAGCGAAGCGCTATGGGCAG
TCAAGCGCTTGATAAGTCTGTATAATTGAGGCTATGAAAATCAAGCCTTCATCCTCATCCAACGCGGCAACAGACGCCGAGACACTGGTTAATTTGACCA
GTGAAATCGCGCGTCTGCAGAGTTTGTTGCAACAGGCTTCTGCAGAGAATCAGGCTTGGTCAGAAAAGTACGGCAAGCTGGAAACCGAGCATACGTCATT
GCGCGAAACCTTCGCCGGGATTCAGCAGCGCTTAGCCTGGTTTGAGAAGCAGCTGTTTGGTCAGAAGTCAGAAAAGCGCGCCCTGGAATTGGGTATGCAG
TTAAGCTTACTGGGTGATATGGTGCCAGCGCTGACCCAGCCTGAAGGCGAAACCGAATATACCACCTACACCCGCAGAAAAGGCAAACAGCGCCCGGATG
ATTGCGTTAATGACAGTGGCCTGCGATTTAACGATAACGTACCGGTGAAAGTGATTACCTTGATACCCGATGAGCTAAAAGGGGAAGAGGCCGACCAGTA
TGAAGTGATTGGCGTAAAAAGCACCTTCCGCTTAGCGCAGCGCCCAGCGAGCTTCGAAGTGCTACGTTATGACCGGCAAATCATCAAACGCAAAGGCAGC
GACACTCTCCTGCCAAGTCAGGCGCCCTTTAATGTATTGGATAAAAGCGTGGCGGATGTGAGCTTTATCGTGGGGATGCTGGTCGATAAATTCCAATACC
ACTTGCCGCTGTATCGCCAGCACCAGCGTTTAGTTGCAGCGGGCATTACTTTAAGCCGCAGCACACTGGGCACCATCGTGGCGCGCGGTATTGATTTACT
CTATCCGATTGTGGATGCGATGCTAAGTAGCATCTTACAAAGCCAGGTGCTGGCAATGGACGAAACGCCGATTAAAGCAGGCAAAGCCGGGCCAGGTAAA
ATGAAGCAAAGTTACTTCTGGCCGGTGTATGGCGATAAGGATGAAATTGTTTTTACCTTCTCCACCAGCCGCGGGCGGCAACATATAGAAGAGATAGTAA
AGCATCGCTTTAAAGGCACCTTATTAAGCGATGGCTACAGCGCCTATGCCAGTTATATCAAAGCCAATGAAGGCTTAACCCATGCCCAGTGCTGGGTGCA
CAGTCGCCGGCAATTTATTGAAGCGGAAAACAGCTGGCCAGCGCCGGCCAAAGAAGCGATTAACCTAATCGCTAAGCTGTATGAGATAGAAGAGACTATC
CGAAACCAAAAGCTCACGGATGACAAAAAACGCCAATACCGCCTTACACACAGCAAACCGGTGGTTGACCGCTTCTTTACGTGGTGTGATGATACCCTGC
ATAACCTGACGCTGCTGCCCAAAGACCCCCTCTCCAAAGCGATTGGCTATGTACAAAGCAAAGAAATGGCACTGCGCGTCTTCCTGGAAGACCCTGATGT
ACCGCTGGATACAAACCATCTGGAGCGGGCGCTTCGGCCCATACCCATGGGGAGAAAAAATTGGTTGTTCTGTTGGACAGAAATCGGTGCAGAGCATGTT
GGCGTTATCCAAAGCCTGATAGTGACCTGCCGCTTACATAATATCAATGTAAACGATTATCTGACCGATGTGCTGCTGCGCATCAGCCAACACCCTGCCA
GCCTGGTGCACGAACTGACGCCACGGTATTGGAAAACTAAGTTCGCTGATAATCCGCTGCGCTCTGACCTCTTTGCTTTACCCACTACTTAGGTAGAATA
GCCTCCAGACGGAGGCGATATGCGCATAGAAGACCTAACCTTAGACGAATTACACGACCTGAACGAGCTTATCTGCAAGCGGATAGGGTACTTGCGTTTG
CAAAACGATATCACTGTTCTAAGCCAGCTACGTTTAGGCCAAAAAGTGCACTTCAAGGCCAAAGAAGGCCAAGTGTTCGGGGTGGTCATCAAAATCAACA
AGAAATCAGTGATGGTGGTCAGCGACGATAATCGCCAGTGGAAAATCCCACCAGGGCTGGTGCAGATCATGAAAGACGTCTAGTCAGGATGGTTGGCCAA
AAACTGCAAGGCGTCAATACGTCCTAAAATGGACGCTTAC
Protein section
ORF number : 4
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 81 | 362 | + | No |
AG : IS66 TnpA
ORF sequence :
MAKRAYRNAAQWQQLIDLWHTSELSITEFCQTHQLSTMSFYKWRQRLASQTEQAPVRQDTQFIDLSALAHQGNTRWHIVLSLGNGVELTLSQQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 351 bp | 116 aa | 362 | 712 | + | No |
AG : IS66 TnpB
ORF sequence :
MFIPAANQKIWLCTTPVDMRKSFNGLSALVRNKLCHNPQSGQYFVFINARKTQMKVLYFESSGYCVWAKRLEQGQFPVKPHPSGIQSLTGCTLQMIVDGI
TVLRHKQAKRYGQSSA
TVLRHKQAKRYGQSSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1659 bp | 552 aa | 734 | 2392 | + | No |
Chemistry : DDE
ORF sequence :
MKIKPSSSSNAATDAETLVNLTSEIARLQSLLQQASAENQAWSEKYGKLETEHTSLRETFAGIQQRLAWFEKQLFGQKSEKRALELGMQLSLLGDMVPAL
TQPEGETEYTTYTRRKGKQRPDDCVNDSGLRFNDNVPVKVITLIPDELKGEEADQYEVIGVKSTFRLAQRPASFEVLRYDRQIIKRKGSDTLLPSQAPFN
VLDKSVADVSFIVGMLVDKFQYHLPLYRQHQRLVAAGITLSRSTLGTIVARGIDLLYPIVDAMLSSILQSQVLAMDETPIKAGKAGPGKMKQSYFWPVYG
DKDEIVFTFSTSRGRQHIEEIVKHRFKGTLLSDGYSAYASYIKANEGLTHAQCWVHSRRQFIEAENSWPAPAKEAINLIAKLYEIEETIRNQKLTDDKKR
QYRLTHSKPVVDRFFTWCDDTLHNLTLLPKDPLSKAIGYVQSKEMALRVFLEDPDVPLDTNHLERALRPIPMGRKNWLFCWTEIGAEHVGVIQSLIVTCR
LHNINVNDYLTDVLLRISQHPASLVHELTPRYWKTKFADNPLRSDLFALPTT
TQPEGETEYTTYTRRKGKQRPDDCVNDSGLRFNDNVPVKVITLIPDELKGEEADQYEVIGVKSTFRLAQRPASFEVLRYDRQIIKRKGSDTLLPSQAPFN
VLDKSVADVSFIVGMLVDKFQYHLPLYRQHQRLVAAGITLSRSTLGTIVARGIDLLYPIVDAMLSSILQSQVLAMDETPIKAGKAGPGKMKQSYFWPVYG
DKDEIVFTFSTSRGRQHIEEIVKHRFKGTLLSDGYSAYASYIKANEGLTHAQCWVHSRRQFIEAENSWPAPAKEAINLIAKLYEIEETIRNQKLTDDKKR
QYRLTHSKPVVDRFFTWCDDTLHNLTLLPKDPLSKAIGYVQSKEMALRVFLEDPDVPLDTNHLERALRPIPMGRKNWLFCWTEIGAEHVGVIQSLIVTCR
LHNINVNDYLTDVLLRISQHPASLVHELTPRYWKTKFADNPLRSDLFALPTT
Blast result :ORF 4
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 87 aa | 2420 | 2683 | + | No |
Annotation : Description :
ORF sequence :
MRIEDLTLDELHDLNELICKRIGYLRLQNDITVLSQLRLGQKVHFKAKEGQVFGVVIKINKKSVMVVSDDNRQWKIPPGLVQIMKDV
Blast result :
Comments
ISAba28 is 97%(orfA), 98%(orfB), 93%(orfC) and 96%(orfD) aa identical to ISSpu21.
References
1] Hamidian M., Hall R.M (2014) Direct submission