ISAba29

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii
DNA section
IS Length : 1309 bp

Ends


IR Length : 26/27

IRL : TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCG
IRR : TGAACCGTACCGGGTTTATCGGAGACTTTTTTATTTAAGTTAGGCCACCT

Insertion site


Left flankDirect repeatRight flankDR Length
GCAGCTTAAAAAGTGTACTTGAT3

DNA sequence

TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCGATGAAAAAACCAAACTATACCCCCGAAATTAGAGAAAGAGCGGTTCAATT
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCAGCAATCACAGCTATTGCTCCTAAAATCGGTTGTACTCCTGAAACATTGCGTGTTTGGTAT
TTAAAGCATATGGATCAACTAAATCCTGCCAAAGTACAACAGATATCTGACCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCAGAACAATAAAGATCGA
TATGGTGTTGAAGCGATTTGTAGAATTTTACCGATTGCAGCTTCGACCTATTATCGGGCTTTAGATCTCGTTGATAACCCAGAACATCGAGCGAAACGTG
CTCTGCATGATTTACATCATGCAGAGCAAATCAAACGTATTTGGAAAGAAAGTTTAGGTCGATATGGTGTGCGTAAGGTCTGGCAACAATTGAAACGTGA
AGGTTATGTTATCGCACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAACGAGCAGATGACTTAGTAAAACGCAATTTTAATGCAGATCATCCTGACCAGCTGTGGGTCGCTGACTTCACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCGCGAGCAATTGTTGGATGGAAAGTATCAACACGTATGAATACAGATATGGTGCTCGATGC
ACTGGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAACGTGATTCATCATAGTGACAGAGGCGTGCAATATCTTTCCATTCGTTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACCGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCGGATTGGCAAGGTTTAGCGGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAGAGCGTGTACACAGTGCACTGGG
TTATGTATCACCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGATAAACCCGG
TACGGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
318 bp105 aa51368+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKPNYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRVWYLKHMDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFAQAEL
DRPHK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
963 bp320 aa3081270+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NSTQSSRFFRPGGARPPTQIMVDFIQNNKDRYGVEAICRILPIAASTYYRALDLVDNPEHRAKRALHDLHHAEQIKRIWKESLGRYGVRKVWQQLKREGY
VIARCTVARLMQKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVADFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALE
QALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYV
SPFEFEAMYYDKINPLGQVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1220 bp406 aa511270+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKPNYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRVWYLKHMDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFRPGGA
RPPTQIMVDFIQNNKDRYGVEAICRILPIAASTYYRALDLVDNPEHRAKRALHDLHHAEQIKRIWKESLGRYGVRKVWQQLKREGYVIARCTVARLMQKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVADFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFEFEAMYYDKIN
PLGQVA

 

Blast result :
Comments
ISAba29 is 97% (orfAB) aa similar to ISAba19.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba29 is also found in the draft genome of Acinetobacter baumannii Naval-83; ctg7180000004880.
References
1] Hamidian M., Hall R.M. Direct submission