ISAba29
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter baumannii | Acinetobacter baumannii |
DNA section
IS Length : 1309 bp
Ends
IR Length : 26/27
IRL : TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCG
IRR : TGAACCGTACCGGGTTTATCGGAGACTTTTTTATTTAAGTTAGGCCACCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAGCTTAAA | AAG | TGTACTTGAT | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGACTCAATATTCTGAGAGACTATTCCGATGAAAAAACCAAACTATACCCCCGAAATTAGAGAAAGAGCGGTTCAATT
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCAGCAATCACAGCTATTGCTCCTAAAATCGGTTGTACTCCTGAAACATTGCGTGTTTGGTAT
TTAAAGCATATGGATCAACTAAATCCTGCCAAAGTACAACAGATATCTGACCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCAGAACAATAAAGATCGA
TATGGTGTTGAAGCGATTTGTAGAATTTTACCGATTGCAGCTTCGACCTATTATCGGGCTTTAGATCTCGTTGATAACCCAGAACATCGAGCGAAACGTG
CTCTGCATGATTTACATCATGCAGAGCAAATCAAACGTATTTGGAAAGAAAGTTTAGGTCGATATGGTGTGCGTAAGGTCTGGCAACAATTGAAACGTGA
AGGTTATGTTATCGCACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAACGAGCAGATGACTTAGTAAAACGCAATTTTAATGCAGATCATCCTGACCAGCTGTGGGTCGCTGACTTCACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCGCGAGCAATTGTTGGATGGAAAGTATCAACACGTATGAATACAGATATGGTGCTCGATGC
ACTGGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAACGTGATTCATCATAGTGACAGAGGCGTGCAATATCTTTCCATTCGTTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACCGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCGGATTGGCAAGGTTTAGCGGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAGAGCGTGTACACAGTGCACTGGG
TTATGTATCACCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGATAAACCCGG
TACGGTTCA
ACTAATTGAATCTGAAAAAGATTATCCTTCTACTTGGGCAGCAATCACAGCTATTGCTCCTAAAATCGGTTGTACTCCTGAAACATTGCGTGTTTGGTAT
TTAAAGCATATGGATCAACTAAATCCTGCCAAAGTACAACAGATATCTGACCAAGAAAAAATGAAGCAAATGGAACGTGAAATTAAAGAATTAAAGCGTG
CCAATGAAATTCTACGCAAAGCAGCCGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTTATCCAGAACAATAAAGATCGA
TATGGTGTTGAAGCGATTTGTAGAATTTTACCGATTGCAGCTTCGACCTATTATCGGGCTTTAGATCTCGTTGATAACCCAGAACATCGAGCGAAACGTG
CTCTGCATGATTTACATCATGCAGAGCAAATCAAACGTATTTGGAAAGAAAGTTTAGGTCGATATGGTGTGCGTAAGGTCTGGCAACAATTGAAACGTGA
AGGTTATGTTATCGCACGTTGTACAGTTGCTCGATTGATGCAGAAGCTAGGTATACAAGGTGTTTGGCGTGGTAAGAATAAACAAACCACCCGTAGCCGA
GATGATCAAAAACGAGCAGATGACTTAGTAAAACGCAATTTTAATGCAGATCATCCTGACCAGCTGTGGGTCGCTGACTTCACGTATATTCAAACTCATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCGCGAGCAATTGTTGGATGGAAAGTATCAACACGTATGAATACAGATATGGTGCTCGATGC
ACTGGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAACGTGATTCATCATAGTGACAGAGGCGTGCAATATCTTTCCATTCGTTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACCGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCGGATTGGCAAGGTTTAGCGGATGTACAACTTGCGACACTAAACTGGGTAGATTGGTTCAATAAAGAGCGTGTACACAGTGCACTGGG
TTATGTATCACCTTTTGAGTTTGAAGCAATGTACTATGATAAGATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAAGTCTCCGATAAACCCGG
TACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MKKPNYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRVWYLKHMDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 963 bp | 320 aa | 308 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
NSTQSSRFFRPGGARPPTQIMVDFIQNNKDRYGVEAICRILPIAASTYYRALDLVDNPEHRAKRALHDLHHAEQIKRIWKESLGRYGVRKVWQQLKREGY
VIARCTVARLMQKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVADFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALE
QALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYV
SPFEFEAMYYDKINPLGQVA
VIARCTVARLMQKLGIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVADFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALE
QALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYV
SPFEFEAMYYDKINPLGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKPNYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPETLRVWYLKHMDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAAFFRPGGA
RPPTQIMVDFIQNNKDRYGVEAICRILPIAASTYYRALDLVDNPEHRAKRALHDLHHAEQIKRIWKESLGRYGVRKVWQQLKREGYVIARCTVARLMQKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVADFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFEFEAMYYDKIN
PLGQVA
RPPTQIMVDFIQNNKDRYGVEAICRILPIAASTYYRALDLVDNPEHRAKRALHDLHHAEQIKRIWKESLGRYGVRKVWQQLKREGYVIARCTVARLMQKL
GIQGVWRGKNKQTTRSRDDQKRADDLVKRNFNADHPDQLWVADFTYIQTHSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFEFEAMYYDKIN
PLGQVA
Blast result :
Comments
ISAba29 is 97% (orfAB) aa similar to ISAba19.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba29 is also found in the draft genome of Acinetobacter baumannii Naval-83; ctg7180000004880.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISAba29 is also found in the draft genome of Acinetobacter baumannii Naval-83; ctg7180000004880.
References
1] Hamidian M., Hall R.M. Direct submission