ISAba24
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP006963 | ND | Acinetobacter baumannii | Acinetobacter baumannii MDR-ZJ06 Acinetobacter baumannii AC29 Acinetobacter baumannii AC12 Acinetobacter baumannii PKAB07 Acinetobacter baumannii TYTH-1 Acinetobacter baumannii MDR-TJ Acinetobacter baumannii AC30 plasmid pAC30b Acinetobacter baumannii NCGM 237 DNA Acinetobacter baumannii BJAB07104 plasmid p2BJAB07104 Acinetobacter baumannii MDR-ZJ06 plasmid pMDR-ZJ06 |
DNA section
IS Length : 2421 bp
Ends
IR Length : 21/22
IRL : GTAAGCGTAACGCCAACCACACATTCCAATCCTAACTGATCAACTTTACC
IRR : GTAAGCGTAACACCAACCACACCTTGCGGTGTGGCTGTTGATCAGTAAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGAATCGTT | TTATCAAG | AACAGATTGA | 8 |
DNA sequence
GTAAGCGTAACGCCAACCACACATTCCAATCCTAACTGATCAACTTTACCCTGTCTGCCATTATCAACTGCCAGGGTAATCATCATGCACCGCACCAAAG
AACAATGGTTCGCTTTATTCGCCGAGCAAGCCCAAAGTGGCCTCACCCAAACTGAATTTGCAAAACGTCAGGGCATCATTCCTACATATTTTAGTTATCG
CAAGAGACAATTGTTGGCGTTTCAGGATGATGACGGTGGCTTTATTGAGCTGACGCCACAGGGCTTGCAGCTGAATAAATCCATTTTACTACGTAAGCGT
GATGTGGAATTGCAGTTTCCAGCCGGCACTGATGTGCTGTGGTTAGCGGCCTTGCTCAGAGCGCTCTGACCATGAAAATGTTTCTTGATGTGCCCCAGGT
CTATTTGCATAAACAGCCGGTCGACTTTCGTAAATCTATTAATGGTTTATCGTTGCTAGTTGAGCAAGAGATGCAGCTCTCGCCTTTTAGTGGCGCACTG
TTTGTATTTTGCAATCAGCAGCGTACCCGGTTAAAAGTACTGTACTGGGATAGCACAGGCTTTTGCTTGTGGTACAAGCGGCTTGAAAAAGACAAGTTCC
GCTGGCCAAGGCAGATGCCTGGCAAAATCCTGTCTATTAGCGAACAACAGTGGCATTGGCTGTTGCAAGGTGTCGATATTCAAAAAATCACCGCCCATCA
GCCACTGCATTACCAGAGTGTGGGCTGATATGACGATCGGCTATAAATCCTTGTGATCGCATCCAAAACAATCTAAATCTTACCAAGCACTTAGTCCAAT
TGGCCGTTACAATAGCGGCCATGAAAAAGACACCTCAATCCTTACCTGATGATGTTGAACTGCTCAAGAAAATGCTGCTTGAGCAGACGCAAAAGCTTGA
GCGGAAACTGGCGCAAAAAGATAAACGCATCGCCAAATTAGAGCGGGTTAACCAGCAACTGTCGGACAAGCTGCAGCAATTGCTGGAGCGTTACAACCTG
AAGAAGTACCAGAAATTTAGCCCCAGCAGCGAGACCTACGAAGGTGATGGCGAAGTCTTAAATGAGGCCGAACAGCTGATTGAAGACGAGTCAGTAGCGA
CAGAGCCAGATAATCAGACGACCGAGAGCGCTGAGAAAGCCGCTAAGCCACGCCGCCCACGGATTGCCCCTGAGCTGCCGCGCGTTGATGTTATCCATGA
TATTGACGATAAAACCTGTACCTGCTGCGGCCATGCGCTGCATCAAATGGGTGAAGAAGTCAGTGAGCAACTCGAGTTCGTCCCAGCACAAATCCGGGTG
CTTAGAAACGTGCGGCTGAAATACAGCTGCCGGCAGTGTGAGACCAACGGCACCAAGGTCGAGATAAAAATTGCCGATGTGCCGCCAGCAGTTATCCCGA
AAAGCATGGCCACACCGAGTCTGCTTGCCCAAATCATCAGCAGCAAAATGCACTATGGCTTGCCGCTGTACCGTCAGGAGCAGTTGTTTAATCAGGCCGG
CATTGAGCTTAGCCGTCAAACGATGAGCCGCTGGCTGATAAGCTGTGCAGAGAAACTGAAACCGTTACTGGCGTTAATGAAAGCTGAGCTTCTGAAACAG
CCGGTGCTATGGGCAGATGAAACCACGGTTAATGTGCTGGATGTTGAGAAAAGCACCTGCTACATGTGGGTATATGGCTGTGGCTTAGAGCAAAGTACTG
GCCCCAAACTGGTGCTGTTTGATTATCAGGATAGTCGCAGCAGCATACATCCCACCGACTTCCTGGCGGGGTTTGCCGGCTATCTGCAGGTTGATGGTTA
TGCCGGATATGAGAAAACCCATGCAACCTTAGTCGGCTGCTGGGCACATGCCAGACGTAAGTTCGTTGAAGCACAAAAAGCGCAGGGTAAAGGCAAAACG
GGTAAGGCGGATGTGGCCCTTAACCATATCCAGAAACTCTATGCACTGGAGAGTCAATTAAAAACCGTGCCGCCTGATAAGAAATATGACGCCAGACAAA
CGCTAGCCAAGCCACTGTTAGAACAATTCAAAAGCTGGCTGGATAAATCAAGCGAGAGTGTCACGAAAGAAAGTTTACTGGGAGCCGCTATTACGTATAG
CCTGAACCAGTGGCCCAAGCTGATGCGCTATCTGGATGATGGCAGGTTGAATATCGACAACAATCGCGCTGAGCGCGCCATCAAACCGTTCGTGATTGGT
CGCAAGGCATGGCTGTTTGCCAATACCAAGTCGGGTGCACAGGCCAGTGCCGCATTGTACAGCTTGGTCGAAACCGCCAAAATCAATGGTCTGGAACCCT
ATGATTACTTATGCCGGTTGCTGACAGAGTTGCCCAAAGCCAATACCCACGAGCAATTGCAACAACTACTGCCTTACTGATCAACAGCCACACCGCAAGG
TGTGGTTGGTGTTACGCTTAC
AACAATGGTTCGCTTTATTCGCCGAGCAAGCCCAAAGTGGCCTCACCCAAACTGAATTTGCAAAACGTCAGGGCATCATTCCTACATATTTTAGTTATCG
CAAGAGACAATTGTTGGCGTTTCAGGATGATGACGGTGGCTTTATTGAGCTGACGCCACAGGGCTTGCAGCTGAATAAATCCATTTTACTACGTAAGCGT
GATGTGGAATTGCAGTTTCCAGCCGGCACTGATGTGCTGTGGTTAGCGGCCTTGCTCAGAGCGCTCTGACCATGAAAATGTTTCTTGATGTGCCCCAGGT
CTATTTGCATAAACAGCCGGTCGACTTTCGTAAATCTATTAATGGTTTATCGTTGCTAGTTGAGCAAGAGATGCAGCTCTCGCCTTTTAGTGGCGCACTG
TTTGTATTTTGCAATCAGCAGCGTACCCGGTTAAAAGTACTGTACTGGGATAGCACAGGCTTTTGCTTGTGGTACAAGCGGCTTGAAAAAGACAAGTTCC
GCTGGCCAAGGCAGATGCCTGGCAAAATCCTGTCTATTAGCGAACAACAGTGGCATTGGCTGTTGCAAGGTGTCGATATTCAAAAAATCACCGCCCATCA
GCCACTGCATTACCAGAGTGTGGGCTGATATGACGATCGGCTATAAATCCTTGTGATCGCATCCAAAACAATCTAAATCTTACCAAGCACTTAGTCCAAT
TGGCCGTTACAATAGCGGCCATGAAAAAGACACCTCAATCCTTACCTGATGATGTTGAACTGCTCAAGAAAATGCTGCTTGAGCAGACGCAAAAGCTTGA
GCGGAAACTGGCGCAAAAAGATAAACGCATCGCCAAATTAGAGCGGGTTAACCAGCAACTGTCGGACAAGCTGCAGCAATTGCTGGAGCGTTACAACCTG
AAGAAGTACCAGAAATTTAGCCCCAGCAGCGAGACCTACGAAGGTGATGGCGAAGTCTTAAATGAGGCCGAACAGCTGATTGAAGACGAGTCAGTAGCGA
CAGAGCCAGATAATCAGACGACCGAGAGCGCTGAGAAAGCCGCTAAGCCACGCCGCCCACGGATTGCCCCTGAGCTGCCGCGCGTTGATGTTATCCATGA
TATTGACGATAAAACCTGTACCTGCTGCGGCCATGCGCTGCATCAAATGGGTGAAGAAGTCAGTGAGCAACTCGAGTTCGTCCCAGCACAAATCCGGGTG
CTTAGAAACGTGCGGCTGAAATACAGCTGCCGGCAGTGTGAGACCAACGGCACCAAGGTCGAGATAAAAATTGCCGATGTGCCGCCAGCAGTTATCCCGA
AAAGCATGGCCACACCGAGTCTGCTTGCCCAAATCATCAGCAGCAAAATGCACTATGGCTTGCCGCTGTACCGTCAGGAGCAGTTGTTTAATCAGGCCGG
CATTGAGCTTAGCCGTCAAACGATGAGCCGCTGGCTGATAAGCTGTGCAGAGAAACTGAAACCGTTACTGGCGTTAATGAAAGCTGAGCTTCTGAAACAG
CCGGTGCTATGGGCAGATGAAACCACGGTTAATGTGCTGGATGTTGAGAAAAGCACCTGCTACATGTGGGTATATGGCTGTGGCTTAGAGCAAAGTACTG
GCCCCAAACTGGTGCTGTTTGATTATCAGGATAGTCGCAGCAGCATACATCCCACCGACTTCCTGGCGGGGTTTGCCGGCTATCTGCAGGTTGATGGTTA
TGCCGGATATGAGAAAACCCATGCAACCTTAGTCGGCTGCTGGGCACATGCCAGACGTAAGTTCGTTGAAGCACAAAAAGCGCAGGGTAAAGGCAAAACG
GGTAAGGCGGATGTGGCCCTTAACCATATCCAGAAACTCTATGCACTGGAGAGTCAATTAAAAACCGTGCCGCCTGATAAGAAATATGACGCCAGACAAA
CGCTAGCCAAGCCACTGTTAGAACAATTCAAAAGCTGGCTGGATAAATCAAGCGAGAGTGTCACGAAAGAAAGTTTACTGGGAGCCGCTATTACGTATAG
CCTGAACCAGTGGCCCAAGCTGATGCGCTATCTGGATGATGGCAGGTTGAATATCGACAACAATCGCGCTGAGCGCGCCATCAAACCGTTCGTGATTGGT
CGCAAGGCATGGCTGTTTGCCAATACCAAGTCGGGTGCACAGGCCAGTGCCGCATTGTACAGCTTGGTCGAAACCGCCAAAATCAATGGTCTGGAACCCT
ATGATTACTTATGCCGGTTGCTGACAGAGTTGCCCAAAGCCAATACCCACGAGCAATTGCAACAACTACTGCCTTACTGATCAACAGCCACACCGCAAGG
TGTGGTTGGTGTTACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 85 | 369 | + | No |
AG : IS66 TnpA
ORF sequence :
MHRTKEQWFALFAEQAQSGLTQTEFAKRQGIIPTYFSYRKRQLLAFQDDDGGFIELTPQGLQLNKSILLRKRDVELQFPAGTDVLWLAALLRAL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 357 bp | 118 aa | 372 | 728 | + | No |
AG : IS66 TnpB
ORF sequence :
MKMFLDVPQVYLHKQPVDFRKSINGLSLLVEQEMQLSPFSGALFVFCNQQRTRLKVLYWDSTGFCLWYKRLEKDKFRWPRQMPGKILSISEQQWHWLLQG
VDIQKITAHQPLHYQSVG
VDIQKITAHQPLHYQSVG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1560 bp | 519 aa | 821 | 2380 | + | No |
Chemistry : DDE
ORF sequence :
MKKTPQSLPDDVELLKKMLLEQTQKLERKLAQKDKRIAKLERVNQQLSDKLQQLLERYNLKKYQKFSPSSETYEGDGEVLNEAEQLIEDESVATEPDNQT
TESAEKAAKPRRPRIAPELPRVDVIHDIDDKTCTCCGHALHQMGEEVSEQLEFVPAQIRVLRNVRLKYSCRQCETNGTKVEIKIADVPPAVIPKSMATPS
LLAQIISSKMHYGLPLYRQEQLFNQAGIELSRQTMSRWLISCAEKLKPLLALMKAELLKQPVLWADETTVNVLDVEKSTCYMWVYGCGLEQSTGPKLVLF
DYQDSRSSIHPTDFLAGFAGYLQVDGYAGYEKTHATLVGCWAHARRKFVEAQKAQGKGKTGKADVALNHIQKLYALESQLKTVPPDKKYDARQTLAKPLL
EQFKSWLDKSSESVTKESLLGAAITYSLNQWPKLMRYLDDGRLNIDNNRAERAIKPFVIGRKAWLFANTKSGAQASAALYSLVETAKINGLEPYDYLCRL
LTELPKANTHEQLQQLLPY
TESAEKAAKPRRPRIAPELPRVDVIHDIDDKTCTCCGHALHQMGEEVSEQLEFVPAQIRVLRNVRLKYSCRQCETNGTKVEIKIADVPPAVIPKSMATPS
LLAQIISSKMHYGLPLYRQEQLFNQAGIELSRQTMSRWLISCAEKLKPLLALMKAELLKQPVLWADETTVNVLDVEKSTCYMWVYGCGLEQSTGPKLVLF
DYQDSRSSIHPTDFLAGFAGYLQVDGYAGYEKTHATLVGCWAHARRKFVEAQKAQGKGKTGKADVALNHIQKLYALESQLKTVPPDKKYDARQTLAKPLL
EQFKSWLDKSSESVTKESLLGAAITYSLNQWPKLMRYLDDGRLNIDNNRAERAIKPFVIGRKAWLFANTKSGAQASAALYSLVETAKINGLEPYDYLCRL
LTELPKANTHEQLQQLLPY
Blast result :
Comments
ISAba24 is 63% (ORFA), 88% (ORFB) and 71% (ORFC : the transposase) aa identical to ISPa30.
References
1] Blackwell G.A.B., Hall R.M (2014) Direct submission