ISAha3

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LN611576 ND Acinetobacter haemolyticus
Acinetobacter haemolyticus 69122-EW plasmid pNDM-69122
DNA section
IS Length : 1039 bp

Ends


IR Length : 15/16

IRL : GGCTTTGTTGCACAAAGCTATTCTTGAAGGCTTCTTCAGTAATATAATTT
IRR : GGCTTTGTTGCATAAACATTCAAAAGCTGAGTTCTCCCCAATCCATTCAA

Insertion site


Left flankDirect repeatRight flankDR Length
TAATTTCCAGTTAGGTGATA0
AGTAAAGGACGTCGGGATCG0

DNA sequence

GGCTTTGTTGCACAAAGCTATTCTTGAAGGCTTCTTCAGTAATATAATTTCCAGATGAAGAAGCCTACACCTAAAATCTATCGTACAACCAATTGGTCCT
CGTATAACCAAGCTTTAATCAAGCGAGGAAATATTTCAATCTGGTTTGATCCTAAGACGCAATGGTATGCACAACCACAAGGCAAGCAAGGACGAAATCA
AACTTATTCCGATACAGCGATTCAATGCTGTTTAATGATCAAATCTCTATTCCGTCTTTCTTTACGTATGGTTACTGGCTTTGTTCAAAGCCTGATTAAA
CTCTGTGGCTTGAATTGGACAGCACCAGATTACTCCACCCTTTGTAGACGACAAAAGCATATTGATATTGCGATAAGCTATCAGAAAAGTCATGATGGGT
TACACCTACTTGTCGACTCTACGGGTTTAAAGTTTTTAGGTGAAGGCGAATGGAAGCGTAAAAAACATCAGCCTGAATACCGTCGGCAATGGCGTAAACT
GCATATTGGTATAGATGCTGAAACACTGCAAATACGTGCCGTTCAGCTTACTACAAATAATGTCAGTGATTCACAAGTACTAGGTGATTTACTGGATCAA
ATTCCACTAGATGAGCGAATAGATTCTGTCTATACCGATGGCGCGTACGACACGAAGTGCTGCAGAAAAGTGATTTCAGATCGTCAAGCACATGCAGTAA
TTCCACCCAGAAAGAATGCCAAGCCCTGGAAAGATTCTAAAATAAGTTCAATAGAACGAAATGAGTTACTTCAAACGGTTAAACATTTAGGTAGAACCCT
ATGGAAAAAATGGTCGGGTTATCACCGTCGTAGTTTGGTGGAAACCAAGATGCATTGCATCAAATTATTAGGTGATAGGCTGACGGCAAGACATTTTCAA
AGTCAGGTCAATGAAATTCATGCGCGTGTGGCAGTTCTGAATAGATTTACGGAATTAGGTAGACCTCGCACCCAAGTTGTGTCTTAAATTTGAATGGATT
GGGGAGAACTCAGCTTTTGAATGTTTATGCAACAAAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
933 bp310 aa55987+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKPTPKIYRTTNWSSYNQALIKRGNISIWFDPKTQWYAQPQGKQGRNQTYSDTAIQCCLMIKSLFRLSLRMVTGFVQSLIKLCGLNWTAPDYSTLCRRQ
KHIDIAISYQKSHDGLHLLVDSTGLKFLGEGEWKRKKHQPEYRRQWRKLHIGIDAETLQIRAVQLTTNNVSDSQVLGDLLDQIPLDERIDSVYTDGAYDT
KCCRKVISDRQAHAVIPPRKNAKPWKDSKISSIERNELLQTVKHLGRTLWKKWSGYHRRSLVETKMHCIKLLGDRLTARHFQSQVNEIHARVAVLNRFTE
LGRPRTQVVS

 

Blast result :
Comments
ISAha3 is 95% aa similar to ISAlw1.
References
1] Jones, L.S., Carvalho, M.J., Toleman, M.A., White, P.L., Connor, T.R., Mushtaq, A., Weeks, J.L., Kumarasamy, K.K., Raven, K.E., Török, M.E., Peacock, S.J., Howe, R.A. and Walsh, T.R. Characterisation of plasmids in extensively drug-resistant (XDR) Acinetobacter species from India and Pakistan. Submitted for publication awaiting final decision.