ISAba21
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| JQ364968 | ND | Acinetobacter baumannii | Acinetobacter baumannii RAB |
DNA section
IS Length : 1274 bp
Ends
IR Length : 28/36
IRL : TGATGTGTGCTGAGAAAACAGGAGGTTTTAACTTGGTAAACTATAGACAC
IRR : TGATATGTGCTGAATTAGCAGGAGACTTTCACTTGGGATATGCTAGGCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCCTAGATAC | ATCTGGCAGT | 0 |
DNA sequence
TGATGTGTGCTGAGAAAACAGGAGGTTTTAACTTGGTAAACTATAGACACTCATCAGGAGTTTACCATGAGCAAGAAACAGAAGACTTACACCGCAGAAT
TTAAAGTTGAAGCAATAAAATTGATTGAAGCCAATCAAGGCAATGTATCGGAAACAGCCAGACAACTTGGCATTTCAATGCAAACTCTTTCAAATTGGAA
TAATAAAGCAAAGACTGGCACCTTAGCAGGCACTAAACAATATTCACCTGATCTAAATGCCTTACTCGAAGAAAATAAAAAGCTCAAACAACAGCTCAAA
ACAGCTGAAATGGAACGTGAATTTCTAAAAAAGGCAGCAGCGTACTTTGCGAAAGAAAGTCAGTAAGGTACGCCTATATGAAACAGCAAAGACATTTATT
CCCGATTAGCTTTATGGCTAGATTACTTCACGTTTCAGTGTCACGATTTTATGATTGGCTAAAACGAGGCATGAATAAAAGATTGGTTCAGCGAAATCAA
CAGACAATTTTGGTCAAAATAGCTCATGAGGAAACTAAACAAAGCTATGGCTATATTCGATTAACTAAGCATTTACAAGCGCAAGGTATCAAAATTAGTA
CGTATGCTGTACGTCAAATAAAAAAGCTCAACCAGCTGTATTGTAAGCGTCATAAGCGTTTCAAAAGAACTACGAAGAGTGATCATAATCGAGCGATCTA
CGCAAATTTACTAGAGCAACAGTTTTCAATGACTAAGCCCAATCTTGCATGGTCAAGCGATATTACATACATTTGGACTGCTGAAGGTTGGTTATACTTG
GCAGCAGTAAAAGACCTGTACACGAAACAAGTGGTTGGCTATAGCTTAAATGAGCGTATGACCGCACAACTTGTTTGCAATGCACTGACTATGGCGATTC
GTAATCAAAAACCAACGAAAGGCTTAATTGTTCATTCGGACAGAGGCAGCCAATATTGCAGCCATGAATATCGAAAGATACTGGAAAAATGTGATTTTCA
AGGTTCAATGAGCAAACGTGGGGACTGTTACGATAATGCACCGATTGAAAGTTTCTGGGGCATACTCAAGAATGAATTAGTGCATCATTGCAACTATAAA
ACCAGAGATGAAGCTAAAGCAGATATTACAAAATACATTGTGTTATTTTATAATCAGCGAAGAATTCAAAAGAGTTTGGATTTTAAAACGCCAATTCAAA
TAGCAGAGAACTTTTATCGGTTAGCTGCCTAGCATATCCCAAGTGAAAGTCTCCTGCTAATTCAGCACATATCA
TTAAAGTTGAAGCAATAAAATTGATTGAAGCCAATCAAGGCAATGTATCGGAAACAGCCAGACAACTTGGCATTTCAATGCAAACTCTTTCAAATTGGAA
TAATAAAGCAAAGACTGGCACCTTAGCAGGCACTAAACAATATTCACCTGATCTAAATGCCTTACTCGAAGAAAATAAAAAGCTCAAACAACAGCTCAAA
ACAGCTGAAATGGAACGTGAATTTCTAAAAAAGGCAGCAGCGTACTTTGCGAAAGAAAGTCAGTAAGGTACGCCTATATGAAACAGCAAAGACATTTATT
CCCGATTAGCTTTATGGCTAGATTACTTCACGTTTCAGTGTCACGATTTTATGATTGGCTAAAACGAGGCATGAATAAAAGATTGGTTCAGCGAAATCAA
CAGACAATTTTGGTCAAAATAGCTCATGAGGAAACTAAACAAAGCTATGGCTATATTCGATTAACTAAGCATTTACAAGCGCAAGGTATCAAAATTAGTA
CGTATGCTGTACGTCAAATAAAAAAGCTCAACCAGCTGTATTGTAAGCGTCATAAGCGTTTCAAAAGAACTACGAAGAGTGATCATAATCGAGCGATCTA
CGCAAATTTACTAGAGCAACAGTTTTCAATGACTAAGCCCAATCTTGCATGGTCAAGCGATATTACATACATTTGGACTGCTGAAGGTTGGTTATACTTG
GCAGCAGTAAAAGACCTGTACACGAAACAAGTGGTTGGCTATAGCTTAAATGAGCGTATGACCGCACAACTTGTTTGCAATGCACTGACTATGGCGATTC
GTAATCAAAAACCAACGAAAGGCTTAATTGTTCATTCGGACAGAGGCAGCCAATATTGCAGCCATGAATATCGAAAGATACTGGAAAAATGTGATTTTCA
AGGTTCAATGAGCAAACGTGGGGACTGTTACGATAATGCACCGATTGAAAGTTTCTGGGGCATACTCAAGAATGAATTAGTGCATCATTGCAACTATAAA
ACCAGAGATGAAGCTAAAGCAGATATTACAAAATACATTGTGTTATTTTATAATCAGCGAAGAATTCAAAAGAGTTTGGATTTTAAAACGCCAATTCAAA
TAGCAGAGAACTTTTATCGGTTAGCTGCCTAGCATATCCCAAGTGAAAGTCTCCTGCTAATTCAGCACATATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 67 | 366 | + | No |
Description : First part of the transposase
ORF sequence :
MSKKQKTYTAEFKVEAIKLIEANQGNVSETARQLGISMQTLSNWNNKAKTGTLAGTKQYSPDLNALLEENKKLKQQLKTAEMEREFLKKAAAYFAKESQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 303 aa | 321 | 1232 | + | No |
Description : Second part of the transposase
ORF sequence :
ISKKGSSVLCERKSVRYAYMKQQRHLFPISFMARLLHVSVSRFYDWLKRGMNKRLVQRNQQTILVKIAHEETKQSYGYIRLTKHLQAQGIKISTYAVRQI
KKLNQLYCKRHKRFKRTTKSDHNRAIYANLLEQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAQLVCNALTMAIRNQKPTK
GLIVHSDRGSQYCSHEYRKILEKCDFQGSMSKRGDCYDNAPIESFWGILKNELVHHCNYKTRDEAKADITKYIVLFYNQRRIQKSLDFKTPIQIAENFYR
LAA
KKLNQLYCKRHKRFKRTTKSDHNRAIYANLLEQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAQLVCNALTMAIRNQKPTK
GLIVHSDRGSQYCSHEYRKILEKCDFQGSMSKRGDCYDNAPIESFWGILKNELVHHCNYKTRDEAKADITKYIVLFYNQRRIQKSLDFKTPIQIAENFYR
LAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 67 | 1232 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKKQKTYTAEFKVEAIKLIEANQGNVSETARQLGISMQTLSNWNNKAKTGTLAGTKQYSPDLNALLEENKKLKQQLKTAEMEREFLKKGSSVLCERKSV
RYAYMKQQRHLFPISFMARLLHVSVSRFYDWLKRGMNKRLVQRNQQTILVKIAHEETKQSYGYIRLTKHLQAQGIKISTYAVRQIKKLNQLYCKRHKRFK
RTTKSDHNRAIYANLLEQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAQLVCNALTMAIRNQKPTKGLIVHSDRGSQYCSH
EYRKILEKCDFQGSMSKRGDCYDNAPIESFWGILKNELVHHCNYKTRDEAKADITKYIVLFYNQRRIQKSLDFKTPIQIAENFYRLAA
RYAYMKQQRHLFPISFMARLLHVSVSRFYDWLKRGMNKRLVQRNQQTILVKIAHEETKQSYGYIRLTKHLQAQGIKISTYAVRQIKKLNQLYCKRHKRFK
RTTKSDHNRAIYANLLEQQFSMTKPNLAWSSDITYIWTAEGWLYLAAVKDLYTKQVVGYSLNERMTAQLVCNALTMAIRNQKPTKGLIVHSDRGSQYCSH
EYRKILEKCDFQGSMSKRGDCYDNAPIESFWGILKNELVHHCNYKTRDEAKADITKYIVLFYNQRRIQKSLDFKTPIQIAENFYRLAA
Blast result :
Comments
ISAba21 is 91% aa similar to ISAba22.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder submission (2014)
2] Bonnin,R.A., Poirel,L. and Nordmann,P. (2012) Int. J. Antimicrob. Agents 40 (3), 257-259.
2] Bonnin,R.A., Poirel,L. and Nordmann,P. (2012) Int. J. Antimicrob. Agents 40 (3), 257-259.