ISCysp20
- Family IS701
- Group ISAba11
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011737 | ND | Cyanothece sp. | Cyanothece sp. PCC 7424 pP742402 |
DNA section
IS Length : 1136 bp
Ends
IR Length : 21/22
IRL : TAGGAGATACGCATTGACAGAATAGCGATGTTATGAGTAATATGACTTTT
IRR : TAGGAGATACGCATTGACAAAAATTTCTACTTATGATGCTAAAGCCTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATAAAGTAAT | AAAAAATAGGT | 0 |
DNA sequence
TAGGAGATACGCATTGACAGAATAGCGATGTTATGAGTAATATGACTTTTCCATAGCTATCGCTGGAGACAAAACCCATAAGACAGCAAACGAAGCCAAG
CACGGCCAAGTGTGACCTCAATATTTACACTAATTATTTAATTGCCGAGCCAAAATGGAGCGGTTGTTCACGTTTAGGGCAAATTATGAATATTTCACAC
GACAGTGCCAATCGTTTTCTGCTTAGGGAACAATATGAGCCGGAAGATCTGTTTGAAGAAATTAAAGCGAGTATTAATTTAATCGGAGGAACATTAAGCG
CTGATGATACAATTGTTGAAAAATTGTACAGTGACTCTTCTAAAGTTGAATTAATAGGGTATTTTTGGTCGGGAAAACACAAAAAGCCTATAAAAGGAAT
TAATTTAATTAGTTTATATTATACGGCTCCAAATGGCGATTCAGTACCTATTAATTATCGTCTTTATAATAAAGAGGAAGGAAAAACAAAAAATGAATAT
TTAAGAGAAATGATCAAGGAGGTTCTGGAATGGGGAGTCATGCCTAAAACAATAACAACCGATAGTTGGTATTCTTCCAAAGATAATTTAAAATTTTTTA
GAGAAAGAAAATTTAATTTTTTGGTGGGAATAGCTAAAAACCGGCAGGTTAAAGTAATGGATGGCAAATTCTGTAAAGTAGAGCAGTTAGACATTCCAGA
AGATGGAATGATAGTTTATTTAAAAGAATTTGGATGGGTAAAAGTATTTCAAAGGGTTTTCAAAAACGAAATACCCCGATACTACGTAATTCATCAAACA
GAAGAGTCGCAATTAGAAGTTTTTACTCGTTCTCAATTTCGCTCTTTATGTTCAATTCATTGGGGCATTGAATGTTATCATAGAGCTTTAAAACAATTAT
GTGGTTTAAGCAAATTTTTAGTGAGAACATCTCAAGCGATCGCTAACCATATTTTTTGTTCTTTAAGAGCTTTTTGCCAACTAGAATTAATGAGAATTCA
AGAAACAATAGAAAATTGGTACGAAATTCAACGAGAGCTTTATTTAAAAGTAGCTAGAGAATTTATTATTAAGCGAGCGCAGGAACAAAAGGCTTTAGCA
TCATAAGTAGAAATTTTTGTCAATGCGTATCTCCTA
CACGGCCAAGTGTGACCTCAATATTTACACTAATTATTTAATTGCCGAGCCAAAATGGAGCGGTTGTTCACGTTTAGGGCAAATTATGAATATTTCACAC
GACAGTGCCAATCGTTTTCTGCTTAGGGAACAATATGAGCCGGAAGATCTGTTTGAAGAAATTAAAGCGAGTATTAATTTAATCGGAGGAACATTAAGCG
CTGATGATACAATTGTTGAAAAATTGTACAGTGACTCTTCTAAAGTTGAATTAATAGGGTATTTTTGGTCGGGAAAACACAAAAAGCCTATAAAAGGAAT
TAATTTAATTAGTTTATATTATACGGCTCCAAATGGCGATTCAGTACCTATTAATTATCGTCTTTATAATAAAGAGGAAGGAAAAACAAAAAATGAATAT
TTAAGAGAAATGATCAAGGAGGTTCTGGAATGGGGAGTCATGCCTAAAACAATAACAACCGATAGTTGGTATTCTTCCAAAGATAATTTAAAATTTTTTA
GAGAAAGAAAATTTAATTTTTTGGTGGGAATAGCTAAAAACCGGCAGGTTAAAGTAATGGATGGCAAATTCTGTAAAGTAGAGCAGTTAGACATTCCAGA
AGATGGAATGATAGTTTATTTAAAAGAATTTGGATGGGTAAAAGTATTTCAAAGGGTTTTCAAAAACGAAATACCCCGATACTACGTAATTCATCAAACA
GAAGAGTCGCAATTAGAAGTTTTTACTCGTTCTCAATTTCGCTCTTTATGTTCAATTCATTGGGGCATTGAATGTTATCATAGAGCTTTAAAACAATTAT
GTGGTTTAAGCAAATTTTTAGTGAGAACATCTCAAGCGATCGCTAACCATATTTTTTGTTCTTTAAGAGCTTTTTGCCAACTAGAATTAATGAGAATTCA
AGAAACAATAGAAAATTGGTACGAAATTCAACGAGAGCTTTATTTAAAAGTAGCTAGAGAATTTATTATTAAGCGAGCGCAGGAACAAAAGGCTTTAGCA
TCATAAGTAGAAATTTTTGTCAATGCGTATCTCCTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 186 | 1106 | + | No |
Chemistry : DDE
ORF sequence :
MNISHDSANRFLLREQYEPEDLFEEIKASINLIGGTLSADDTIVEKLYSDSSKVELIGYFWSGKHKKPIKGINLISLYYTAPNGDSVPINYRLYNKEEGK
TKNEYLREMIKEVLEWGVMPKTITTDSWYSSKDNLKFFRERKFNFLVGIAKNRQVKVMDGKFCKVEQLDIPEDGMIVYLKEFGWVKVFQRVFKNEIPRYY
VIHQTEESQLEVFTRSQFRSLCSIHWGIECYHRALKQLCGLSKFLVRTSQAIANHIFCSLRAFCQLELMRIQETIENWYEIQRELYLKVAREFIIKRAQE
QKALAS
TKNEYLREMIKEVLEWGVMPKTITTDSWYSSKDNLKFFRERKFNFLVGIAKNRQVKVMDGKFCKVEQLDIPEDGMIVYLKEFGWVKVFQRVFKNEIPRYY
VIHQTEESQLEVFTRSQFRSLCSIHWGIECYHRALKQLCGLSKFLVRTSQAIANHIFCSLRAFCQLELMRIQETIENWYEIQRELYLKVAREFIIKRAQE
QKALAS
Blast result :
Comments
ISAba11-like insertion sequence present as a single-copy on the plasmid pP742402. This IS element is a member of a small and newly described IS family that has diverged from the IS4 family. See Rieck et al. (manuscript submitted) for further details.
ISCysp20 is 76% aa similar to ISAcma42.
ISCysp20 is 76% aa similar to ISAcma42.
References
1] Kumar Rajakumar (2011) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2008) Direct submission to GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2008) Direct submission to GenBank.