ISHbo3
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001690 | ND | Halogeometricum borinquense | Halogeometricum borinquense DSM 11551 |
DNA section
IS Length : 1471 bp
Ends
Left end : AGAAAAGATCAAGGCGGATGCCCCGAGGCTTGACCTCGGGGAGGAAGCCGACAACTAAGGGCACAAACCACGTTACGAAGGTAGGCTACCTCTCAGCGAT II struct. : Yes
Right end : TCTTGGCAGTTAGACCGGGAGTCCACGCGAAAGCCTCGGGGTCGAACGGAAATCTTTGATTTCCGTGATGACGAGACGCTACGCGTCTCGAACCACTTGA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CCGGTGATAAAC | TTCAC | ACGGAACGTTCG | ttga |
DNA sequence
AGAAAAGATCAAGGCGGATGCCCCGAGGCTTGACCTCGGGGAGGAAGCCGACAACTAAGGGCACAAACCACGTTACGAAGGTAGGCTACCTCTCAGCGAT
ATGTGAAACACAGTGGAATACCGTCGAACCGCCGTCATCAAACTCGACACTCCCAAAGGAGCAAATCCGATTCTCCATGAGACTGTCGAGCAGTTCAAAC
ACTGCGCCAACACCGCAAGCGAGTGGTGCTGGCACGGGCGTCGCCAGATTCCTGTAGAATCTGGCTGCCAACCAGAAACGCGCGGCGTTTCTAGTGACGA
CGACGGATACCACGTCACATCCAAAGCCAAAGCCGAACAAGCTCTCTACGACCAACTCCGCAACGAAACCAACCTCACCGCGAATCTCATCCAGAAAGGG
ATTCGACGGGCGGTTGAAGCCGTCAAAAGCGGAGTTGAACGCCTCAAGCGTGGCGAGAGAACCTCACGACCCTACTTCTCAGCAGATAGCGCCGTGTACG
ACAAGCGAAGTGCAACGTTCCACCGCGACCACGTTTCCCTCTCTACCGTTGACGGACGTATCGAGTGCGATTACATCCTTCCCGACGACTCGGAGAAACC
ACCAACGAAGTACGTCTCCGACGAGGATTTCGAGTTTCGGATGGCGCACTTACAGTACCGCGACGGCGACTGGTACTTGCACGCTTCCATGCGGAAGGTC
GAAGAAAGCGACGACCCCGAATCCGATTCCAAGCACAGAACAGTTCTTGGTGTGGATTTGGGCGTGAACAACCTCGCCGTCGCTTCAACAGGGCGCTTCT
GGTCGGCAGACGAGTTCAACCACTGGCGCAGAGAGTACGAGAAACGCCGTGGATCGCTCCAACAGTGCGGCTCGCGTCACGCTCACGAGAACATCGAGAG
TGTCGGTCACAAAGAGACGGGGAGATTCGAGATATATCTGCACTCGGTGGCGAACGAACTCATCGAGGAGGCCGTCGAATACGACTGCTCGCATATCGTG
TTCGAGGACTTGACCCACATTCGGGAGAACATCCCGAAGGCGACGTGGCAACACGTATGGGCGTTCCGACGCCTCTACGAATACGTCGAATACAAAGCTG
AGGAGTACGGCGTGGAAGCCGTACAGGTTGACCCACGCAACACGTCGAAGCGGTGTTCGACGTGTGGGTTTACCCATAGCGACAATCGGTCTGGGGAAGC
GTTCTGTTGTCAGAAGTGTGGGTACGAGAACCACGCTGACTACAACGCTTCCAAGAACATCGGTTTGCAGTATCTCCGTCGTCGGCAAAACGCGGACGAT
GGAGGCGCATCCGTAGATGTGCGCTTGAATCGCGGGACGCTGAACGTGAACGGCGAATATTCGTCTACTGCTCTTGGCAGTTAGACCGGGAGTCCACGCG
AAAGCCTCGGGGTCGAACGGAAATCTTTGATTTCCGTGATGACGAGACGCTACGCGTCTCGAACCACTTGA
ATGTGAAACACAGTGGAATACCGTCGAACCGCCGTCATCAAACTCGACACTCCCAAAGGAGCAAATCCGATTCTCCATGAGACTGTCGAGCAGTTCAAAC
ACTGCGCCAACACCGCAAGCGAGTGGTGCTGGCACGGGCGTCGCCAGATTCCTGTAGAATCTGGCTGCCAACCAGAAACGCGCGGCGTTTCTAGTGACGA
CGACGGATACCACGTCACATCCAAAGCCAAAGCCGAACAAGCTCTCTACGACCAACTCCGCAACGAAACCAACCTCACCGCGAATCTCATCCAGAAAGGG
ATTCGACGGGCGGTTGAAGCCGTCAAAAGCGGAGTTGAACGCCTCAAGCGTGGCGAGAGAACCTCACGACCCTACTTCTCAGCAGATAGCGCCGTGTACG
ACAAGCGAAGTGCAACGTTCCACCGCGACCACGTTTCCCTCTCTACCGTTGACGGACGTATCGAGTGCGATTACATCCTTCCCGACGACTCGGAGAAACC
ACCAACGAAGTACGTCTCCGACGAGGATTTCGAGTTTCGGATGGCGCACTTACAGTACCGCGACGGCGACTGGTACTTGCACGCTTCCATGCGGAAGGTC
GAAGAAAGCGACGACCCCGAATCCGATTCCAAGCACAGAACAGTTCTTGGTGTGGATTTGGGCGTGAACAACCTCGCCGTCGCTTCAACAGGGCGCTTCT
GGTCGGCAGACGAGTTCAACCACTGGCGCAGAGAGTACGAGAAACGCCGTGGATCGCTCCAACAGTGCGGCTCGCGTCACGCTCACGAGAACATCGAGAG
TGTCGGTCACAAAGAGACGGGGAGATTCGAGATATATCTGCACTCGGTGGCGAACGAACTCATCGAGGAGGCCGTCGAATACGACTGCTCGCATATCGTG
TTCGAGGACTTGACCCACATTCGGGAGAACATCCCGAAGGCGACGTGGCAACACGTATGGGCGTTCCGACGCCTCTACGAATACGTCGAATACAAAGCTG
AGGAGTACGGCGTGGAAGCCGTACAGGTTGACCCACGCAACACGTCGAAGCGGTGTTCGACGTGTGGGTTTACCCATAGCGACAATCGGTCTGGGGAAGC
GTTCTGTTGTCAGAAGTGTGGGTACGAGAACCACGCTGACTACAACGCTTCCAAGAACATCGGTTTGCAGTATCTCCGTCGTCGGCAAAACGCGGACGAT
GGAGGCGCATCCGTAGATGTGCGCTTGAATCGCGGGACGCTGAACGTGAACGGCGAATATTCGTCTACTGCTCTTGGCAGTTAGACCGGGAGTCCACGCG
AAAGCCTCGGGGTCGAACGGAAATCTTTGATTTCCGTGATGACGAGACGCTACGCGTCTCGAACCACTTGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1272 bp | 423 aa | 113 | 1384 | + | No |
AG : TnpB
ORF sequence :
MEYRRTAVIKLDTPKGANPILHETVEQFKHCANTASEWCWHGRRQIPVESGCQPETRGVSSDDDGYHVTSKAKAEQALYDQLRNETNLTANLIQKGIRRA
VEAVKSGVERLKRGERTSRPYFSADSAVYDKRSATFHRDHVSLSTVDGRIECDYILPDDSEKPPTKYVSDEDFEFRMAHLQYRDGDWYLHASMRKVEESD
DPESDSKHRTVLGVDLGVNNLAVASTGRFWSADEFNHWRREYEKRRGSLQQCGSRHAHENIESVGHKETGRFEIYLHSVANELIEEAVEYDCSHIVFEDL
THIRENIPKATWQHVWAFRRLYEYVEYKAEEYGVEAVQVDPRNTSKRCSTCGFTHSDNRSGEAFCCQKCGYENHADYNASKNIGLQYLRRRQNADDGGAS
VDVRLNRGTLNVNGEYSSTALGS
VEAVKSGVERLKRGERTSRPYFSADSAVYDKRSATFHRDHVSLSTVDGRIECDYILPDDSEKPPTKYVSDEDFEFRMAHLQYRDGDWYLHASMRKVEESD
DPESDSKHRTVLGVDLGVNNLAVASTGRFWSADEFNHWRREYEKRRGSLQQCGSRHAHENIESVGHKETGRFEIYLHSVANELIEEAVEYDCSHIVFEDL
THIRENIPKATWQHVWAFRRLYEYVEYKAEEYGVEAVQVDPRNTSKRCSTCGFTHSDNRSGEAFCCQKCGYENHADYNASKNIGLQYLRRRQNADDGGAS
VDVRLNRGTLNVNGEYSSTALGS
Blast result :
Comments
ISHbo3 is 88% aa (TnpB) similar to ISNpe12.
References
1] Pfeiffer F. (2013) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Anderson,I., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Anderson,I., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.