ISSal1

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Shewanella algae
Shewanella algae ACDC
DNA section
IS Length : 2528 bp

Ends


IR Length : 16/23

IRL : GTAACCGTCCGCCGAACCCATCTCCCCGCTTCTTCCAGATAGCGGCATGG
IRR : GTAAGCGTCTAGCTAACTCACCTTGCGTATCTGTATCACAGGCTGCACGG

Insertion site


Left flankDirect repeatRight flankDR Length
GGCGCTCGCGGCTATTTTTTTGTTAGCC8

DNA sequence

GTAACCGTCCGCCGAACCCATCTCCCCGCTTCTTCCAGATAGCGGCATGGTGTGCACCTATTTTTGGTGGACACCATTTCAAGCCACTTTCTGGAGGGTT
TCGTGTCTCGACAGCGCCGTACATTCCCCAAGGCTTTCAAGGCCCAGGTCATCGAAGAGTGCGCCCAGTCCGGTGCTTCCGTCGCCGGCGTGGCGCTCAG
CCACGGGCTCAATGCCAACCTGGTGCATAAATGGATTCGTAAGCAGCGCGAGCCGCTACCGGTCGTATCTGCACGCTTTATCCCGGTGCCGCTCGGCATC
CTGCCTTCAGAGCAGGCCAGCTCTGAAGAGCTGACGATACGAATCGAAATCCCCTACCACGCCGGCAGTCTGTCGGTGCACTGGCCGGTGCAGGACGGCG
AAGGCTGTACACGCTTGCTGCGCGGGCTGCTCGCATGATTCGCATCGATCGCATCTGGCTGGCCACCGAGCCGATGGACATGCGCGCCGGCACCGAAACC
GCATTGGCCCGTGTAGTGGCGGTATTCGGTGCGGCCAAGCCGCACTGTGCTTATCTGTTCGCCAACCGCCGCGCCAACCGCATGAAAGTGTTGGTGCATG
ACGGTGTAGGGATCTGGCTGGCCGCGCGGCGATTGAACCAAGGCAAGTTTCACTGGCCAGGCATCCACCGCGGCTTGGAAGTTGAACTCGACACCGAGCA
GCTTCAGGCTTTGGTGCTCGGTTTACCCTGGCAGCGGGTTGGTGCAGCCGGTGCGATCACAATGCTGTAGCGCCGGCCATTAGCCTATCGCTTTATCTGC
GCGGATCGCCTGCTCTGGCACAATCAGCGGCATGACTTCCTTGCCCAATCTCGACCAACTAACACCTGAGCAACTGCGCGCACTGGCCGCCCAGTTGCTC
ACGCAGGTCGATGCGATGGGCAAAAAGATCCACCGTGATCAAACCATCATCGAGCAGCTCACCCACGAAATCGCCTGGTTCAAGCGCCACAAGTTCGCCA
AGCGTAGCGAGCAACTAAGCCCTGAACAGGGCAGCCTGCTGGATGATTTGCTCGACACGGACATCGCCGCCATCGAGGCGGAACTGAAAACCCTCAATCC
TCCGGCGGCTCCGGCTGAACCCCGCCAACAGCCCAAGCGCGCGCCGCTGCCAGCCCAGTTTCCGCGTACTGTGATCCGTCACGAGCCGCAGAACACTCAA
TGCGCCTGCGGCTGCCCGCTGCAACGCATCGGCGAAGACATCAGCGAAAAGCTGGATTACACGCCTGGCGTGTTCACCGTCGAACAGCATGTGCGTGGCA
AGTGGGCCTGCCGCCAGTGCGAAACATTGATCCAGGCGCCGGTACCAGCCCATGTGATCGACAAGGGCATCCCGACTGCCGGCTTGCTGGCTCATGTCAT
GGTGGCCAAATACGCCGATCATCTGCCGCTGTACCGGCAAGAGAAGATCTTTGGCCGTGCGGGTCTGGCTATCGCGCGTTCGACATTGGCGCAGTGGGTC
GGACAAACCGGCGTACAGCTCCAACCATTGGTCGATGCCCTGCGCGAGGCGGTACTGGCGCAAGGCGTGATCCATGCCGACGAAACCCCCGTGCAAATGC
TGGCCCCTGGCGAGAAGAAAACCCACCGGGCTTACGTCTGGGCATACTGCACCACACCCTTCGCCGACCTGAAGGCCGTGGTCTATGACTTCAGCCCAAG
CCGTGCAGGTGAGCATGCGCGCAATTTTATGGGTCAGTGGAATGGCAAACTGGTCTGCGACGACTTCGCCGGCTACAAGGCCAGCTTCGAGCAGGGCATC
ACCGAAATTGGCTGCATGGCCCACGCCCGCCGCAAATTTTTCGATCTGCACTCAGCGAACAAAAGCCAGTTAGCCGAACAGGCGCTGCACTCCATTGCCG
GGCTGTATGAAATCGAGCGGCAAGCACGGCACATGAGCGATGAAGAACGCTGGCGAATACGCCAGGAAAAGTCTTCACCGATCCTCGATGCGCTGCATGA
CTGGATGCTGGCCCAGCGTGATCGGGTGCCCAATGGATCAGCCACGGCAAAAGCCCTGGATTACAGCCTAAAACGCTGGCTAGCGCTGACGCGCTATCTG
GAAGATGGAGCCGTGCCCATCGACAACAATCAGGTCGAGAACCAGATTCGGCCTTGGGCATTGGGGCGCTCGAACTGGCTGTTTGCCGGTTCACTACGCA
GCGGCAAACGGGCGGCTGCGATCATGAGCCTGATCCAGTCAGCACGCCTCAACGGTCACGATCCGTACGCCTACCTGAAGGACGTGCTCATGCGGCTACC
AACGCAGCGGGCAAGCGAAATCAGTCAACTGCTGCCGCATCAGTGGATACAGCCTGAAGCAGGCATGTAACCACAGCGGTGTATCACCCCAAACAGCCTA
CCCGTAATAGTCTCGCACTGATTTTTTGGGCGTTCACGATGTGATACACGGTAAGCGTTCAGCGTGTGATACAGCTACCCGTGCAGCCTGTGATACAGAT
ACGCAAGGTGAGTTAGCTAGACGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
387 bp128 aa52438+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

VHLFLVDTISSHFLEGFVSRQRRTFPKAFKAQVIEECAQSGASVAGVALSHGLNANLVHKWIRKQREPLPVVSARFIPVPLGILPSEQASSEELTIRIEI
PYHAGSLSVHWPVQDGEGCTRLLRGLLA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
336 bp111 aa435770+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRIDRIWLATEPMDMRAGTETALARVVAVFGAAKPHCAYLFANRRANRMKVLVHDGVGIWLAARRLNQGKFHWPGIHRGLEVELDTEQLQALVLGLPWQ
RVGAAGAITML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1539 bp512 aa8322370+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTSLPNLDQLTPEQLRALAAQLLTQVDAMGKKIHRDQTIIEQLTHEIAWFKRHKFAKRSEQLSPEQGSLLDDLLDTDIAAIEAELKTLNPPAAPAEPRQQ
PKRAPLPAQFPRTVIRHEPQNTQCACGCPLQRIGEDISEKLDYTPGVFTVEQHVRGKWACRQCETLIQAPVPAHVIDKGIPTAGLLAHVMVAKYADHLPL
YRQEKIFGRAGLAIARSTLAQWVGQTGVQLQPLVDALREAVLAQGVIHADETPVQMLAPGEKKTHRAYVWAYCTTPFADLKAVVYDFSPSRAGEHARNFM
GQWNGKLVCDDFAGYKASFEQGITEIGCMAHARRKFFDLHSANKSQLAEQALHSIAGLYEIERQARHMSDEERWRIRQEKSSPILDALHDWMLAQRDRVP
NGSATAKALDYSLKRWLALTRYLEDGAVPIDNNQVENQIRPWALGRSNWLFAGSLRSGKRAAAIMSLIQSARLNGHDPYAYLKDVLMRLPTQRASEISQL
LPHQWIQPEAGM

 

Blast result :
Comments
ISSal1 is 61% (orfA), 93% (orfB) and 91%(orfC, the transposase) aa similar to ISPpu14.
References
1] Iain C. Clark (2014) Direct submission to ISfinder
2] Iain C. Clark, Ryan A. Melnyk, Anna Engelbrektson, John D. Coates (2013) mBio. Jul-Aug; 4(4): e00379-13