ISHvo17

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001956 ND Haloferax volcanii
Haloferax volcanii DS2
DNA section
IS Length : 1508 bp

Ends


Left end : ACATGGCGAGGAGAATACCCGCGCCTAAAGGCGCGGGATTAATCCGTCAACCCCTCCACTAACCACGTTCGATAGCACGCTCGTTACTTTTAATATACTA II struct. : Yes
Right end : TGCGGTCGGGATTCCCGCGTTGACGAGACGCTCTGCGTCTCGTTCGCACACCAGAACACTCTGCGTTCTGGGGACGTCTTTAGGCACGGGAGGATGTCAA II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CCGCGCGAACTTATATCTCGTCAGtcaa

DNA sequence

ACATGGCGAGGAGAATACCCGCGCCTAAAGGCGCGGGATTAATCCGTCAACCCCTCCACTAACCACGTTCGATAGCACGCTCGTTACTTTTAATATACTA
ATTCTTATAAGTATTTATAACCATAGTCAGATGCTGGAGACAACCCGAACCTATCGAGCGACAATCGTCAATCACTCGCAAGTGAGTGACGACCTCGATG
ACTGCGGACACTCCGCGTCGAAACTGTGGAACGTCGCCCGCTACCACATCCAGCAAGAATGGAACGATACCGGCGAGATTCCGTCTGAAGCCGACCTCAA
GCGCGAATTAAAAGACCACGAACGATATAGTGACCTCCATTCTCAGTCAAGTCAGCGCGTTCTCGAAGAACTCGCTGAGTCGTTCACCGGTTGGTTCAAA
AAGCGCAAGAACGGCGACACAGATGCGAATCCGCCCGGCTACCGAAAGCGAGGCGACAACCACCCACGCTCCACCGTGACGTGGAAGCAGCACGGCATCA
AACACGACACCTCGAACAATAGGCTCCGTCTCAGCAAGGGGTTCAACCTCAAAAGCCACCGTTCGGACTTCATCCTCTGCGAGTACGAGACGCGTCCGGA
CGTGACCGTAGAGAACATCCAGCAAGTGCGAGCCGTATGGAACGGCGACAGATGGGAACTGCACCTCGTCTGCAAGGTCGAAATCCCCGTCGAGGACGCT
CCCGGCGATAACGCCGCAGGTATCGACCTCGGCATCAAGAACTACCTCGCCATCGCTTACGACGACGGTGAGGCGGAGTTGTATTCGGGGAACGTGCTGA
AACAGGACAAACACTACTTCACCCGTGACGAGTACGATACCGAAGGCGAGAACGGGCCGTCACGTCGTGCGCTTCGCGCTCGCCAGAAGCTCTCGCGTCG
GAAAGACCACTTCCTGCACGCACTCTCCAAGCACATCGTCGAGCAGTGTATCGACCACGAGGTCGGTCGTATCGCCGTCGGTGACTTGAGCGATATTCGA
GAGGATGAAAATGGTGACTCTCGGAATTGGGGTACACGTAGAAATAAGAAACTCCACGGCTGGGAGTTCGACCGGTTCACTCGTCTGCTCGAATACAAGG
CCGAAGAACACGGGATTCTCGTAGACCGCAAGAGTGAGCGAGATACGTCAAAGACGTGTTCGTGCTGTGGGCGGAAGCGTGAGGCGAAGCGCGTGGAGCG
TGGGTTGTACGTCTGTGAGTCGTGCGGTGTGACGATGAACGCGGACGTGAACGGTGCGGTCAACATTCGCAGAAAGATAACTCAGAATCCTCCGACGGAG
GATATGAGTAACGGTCGTTTGGCACGGCCAGTAGCCTACCTGTTCACTCAAACCTCGGGGCGTTTTGCACCGAGCGAGCAGGCGGGTTGCAAACCCTAAT
ATCCCAACTGCGGTCGGGATTCCCGCGTTGACGAGACGCTCTGCGTCTCGTTCGCACACCAGAACACTCTGCGTTCTGGGGACGTCTTTAGGCACGGGAG
GATGTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1269 bp422 aa1311399+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLETTRTYRATIVNHSQVSDDLDDCGHSASKLWNVARYHIQQEWNDTGEIPSEADLKRELKDHERYSDLHSQSSQRVLEELAESFTGWFKKRKNGDTDAN
PPGYRKRGDNHPRSTVTWKQHGIKHDTSNNRLRLSKGFNLKSHRSDFILCEYETRPDVTVENIQQVRAVWNGDRWELHLVCKVEIPVEDAPGDNAAGIDL
GIKNYLAIAYDDGEAELYSGNVLKQDKHYFTRDEYDTEGENGPSRRALRARQKLSRRKDHFLHALSKHIVEQCIDHEVGRIAVGDLSDIREDENGDSRNW
GTRRNKKLHGWEFDRFTRLLEYKAEEHGILVDRKSERDTSKTCSCCGRKREAKRVERGLYVCESCGVTMNADVNGAVNIRRKITQNPPTEDMSNGRLARP
VAYLFTQTSGRFAPSEQAGCKP

 

Blast result :
Comments
ISHvo17 is 84% (TnpB) aa similar to ISHxa1.
Comparing to ISH35, there is an insertion of 57 nts (TGACGAGACGCTCTGCGTCTCGTTCGCACACCAGAACGCTGTGCGTTCTGGGGACGA) inside the rigth end of the sequence. This inactive the RE.
References
1] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605