ISHvo11

  • Family IS4
  • Group ISH8
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001955 ND Haloferax volcanii
Haloferax volcanii DS2
Haloferax volcanii DS2p plasmid pHV4
Haloferax volcanii_DS2p plasmid pHV1
DNA section
IS Length : 1445 bp

Ends


IR Length : 15/16

IRL : CATCCGTTCTAGTTTAACGAAGAATCCAGTTGCTGTCTGTTGATTTGATA
IRR : CATGCGTTCTAGTTTAGTAGCGATCAAAGCCGAACTCACCTCGTTCAACC

Insertion site


Left flankDirect repeatRight flankDR Length
GCCCGATATTAGCCGGCGAGAGGTGATGCAACAGACACTTGCTG0
CGAGGGAGAAGAGTGATGCAACCCGGCGAGAGTTCGCTGATATC0
CGTCGAATCGAGGCGGTGGGTCCATCCGTTCTAG10
CTAGAACGCATGGCGGTGGGTCAAAGAAAATGAA10
TGAGAACACTAAGGCCGAAGAGGAAACTGCAGCG10
GGTAACTCCCGACCGGGCTAGCCAACAGTGGGAG10
CGAATCCGATTGGAGCAGGTCCATGATGATACTC10
ACAGGTTCGCTGGCGTGACGGCGTCGAATGCCCC10
TCAGCGCGCAACACAATAACATACAACAACTGTG10
TAGGGGTCGGACCCCCGAAGATGA0
TCCACGCGTACGGCTGGCCCACGA0
TTATGTCGGGGCGAACTACGACCA0
ATGTTTGATATGGTGACGGATGGT0
TGGGTATCCAGTGGTACCAGCCAT0
TCGTCACCATAGAGGCTACAACAA0

DNA sequence

CATCCGTTCTAGTTTAACGAAGAATCCAGTTGCTGTCTGTTGATTTGATATCGTCGCAACTACCTGATTGAGAAACCCGGTGGGAAGTATGACCTACAGC
CCACCGGATTCGGTCATAGTTGACCGGATTCAAAGAGCGTTTCCCTCTGATGAGTTGCGCGAGCGCGCTCGCGCAACGAATCTCGTCCAACGAGAGCGGA
AATTCGACATCGTTGCGCTGTTCTACACACTCTCGTTTGGCTTCGCTGCTGGCTCAGACCGCTCTCTCCAAGCATTTCTCGAACGCTACGTCGAGATGGC
TGACTGTGACGAACTCTCCTACGCATCGTTCCACGACTGGTTCGAACCAGGATTCGTTGCACTCCTTCGAGAGATTCTCGATGACGCAATCGAGAATCTC
GATACCGGACGAGAAGATTTGAACGGCCGTCTCGAACGCTTTCGAGACGTCCTCATTGCTGACGCAACCATCGTTTCGCTGTACCAGGACGCCGCTGATA
TCTACACAGCAACCGGCGACCATCAAGCCGAACTGAAACTTCACCTCACCGAATCTCTCTCGACTGGGCTCCCGACACGATTCCGGACAACCGATGGGAC
GACTCATGAACGGAGTCAGCTACCCACCGGCGAGTGGGTAGCTGACGCCCTCATCTTGCTCGATTTAGGCTTCTACGACTTCTGGTTGTTCGACCGAATC
GACCAGAACGGCGGGTGGTTCGTCTCCCGGGTGAAGGACAACGCGAACTTCGAGATCGTCGAAGAACTGCGAACGTGGCGAGGCAACAGCATTCCGCTGG
AAGGAGAGTCGCTGCAGGCCGTCCTTGAGGACCTGCAGCGACAGGAAATCGACGTACGCATCACGCTTTCATTCGAGCGCAAACGAGGGTCGGGCGCCAG
CGCGACCCGGTCGTTCCGATTGGTCGGCCTGCGTAACAAGGAGAGCGAAGAGTACCATCTGTATCTGACGAATCTGGCGAGAGAAAGCTACAGCGCGCCC
GATATCGCGCAGCTCTATCGGGCGCGCTGGGAGGTCGAACTGCTGTTCAAGGAGTTGAAGTCGCGGTTCGGCTTGGACGAGATCAAGACGACCGACGGCT
ACATCATCGAGGCGCTGATCATCATGGCCGCAATTTCGTTGATGATGAGTCGTGTAATCGTGGATGAGTTACGGTCGCTCGAGGCAAGACAGCGAGAGGG
CGAAGCCGCCGCAGACGCCGACTCGTCGGCGTCGCGGCTCCCTCGGCGTCGCTGTTCGCTCGCCGTGGAACGCCACGGTCATCTAATCCAGTTGTATCTC
ATGATCGAGTTGGGCTACGAACTGCCGGATTTGGACGAGCTGTTGCTGTGGGCGTCGCGTAATCCAAATCCACACAGGCAACGGTTACGTGAGCAGGTTG
AACGAGGTGAGTTCGGCTTTGATCGCTACTAAACTAGAACGCATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1350 bp450 aa801429+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGSMTYSPPDSVIVDRIQRAFPSDELRERARATNLVQRERKFDIVALFYTLSFGFAAGSDRSLQAFLERYVEMADCDELSYASFHDWFEPGFVALLREIL
DDAIENLDTGREDLNGRLERFRDVLIADATIVSLYQDAADIYTATGDHQAELKLHLTESLSTGLPTRFRTTDGTTHERSQLPTGEWVADALILLDLGFYD
FWLFDRIDQNGGWFVSRVKDNANFEIVEELRTWRGNSIPLEGESLQAVLEDLQRQEIDVRITLSFERKRGSGASATRSFRLVGLRNKESEEYHLYLTNLA
RESYSAPDIAQLYRARWEVELLFKELKSRFGLDEIKTTDGYIIEALIIMAAISLMMSRVIVDELRSLEARQREGEAAADADSSASRLPRRRCSLAVERHG
HLIQLYLMIELGYELPDLDELLLWASRNPNPHRQRLREQVERGEFGFDRY

 

Blast result :
Comments
ISHvo11 is 96% aa similar to ISH5.
A tandem pair of ISHvo11A transposons (97% seq_id) is separated by 10 bp (GCGGTGGGTC) which are a target duplication for each of the elements.
A 230 kb inversion between an oppositely oriented pair of ISHvo11 transposons has swapped the adjacent sequences (CCGGCGAGAG,GTGATGCAAC) which initially were target duplications : GCCCGATATTAG (CCGGCGAGAG/GTGATGCAAC) AGACACTTGCTG ; CGAGGGAGAAGA (GTGATGCAAC/CCGGCGAGAG) TTCGCTGATATC.
File updated the 29/01/2015 with new insertion sites.
References
1] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605