ISHhu2

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
LN831303 ND Halobacterium hubeiense
Halobacterium hubeiense
DNA section
IS Length : 1846 bp

Ends


Left end : AGCGAGAGAATCCTACCCTTTAGGGCGGGCGTGAATCGCGTACGCTCCGTGCCACGGACCACCGTCGGTTGCAGGCCGAATACCCCAGGACTGCAACCCT II struct. : Yes
Right end : GCTCACGGCCTACGAAGAGTCGGAACCCTCTGCCAGCGACGACTGACTGGTATTCCCCATGCGTGGGAAGCCTCGCCGTTTACGGCGAGGAGGATGTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
ATTCGGCGACAGCTATCTTCTAACCGGGAGCCGATGTCAC

DNA sequence

AGCGAGAGAATCCTACCCTTTAGGGCGGGCGTGAATCGCGTACGCTCCGTGCCACGGACCACCGTCGGTTGCAGGCCGAATACCCCAGGACTGCAACCCT
TTATTAGAATTGGGTGTATAGAAATCAATACGGCTAAATGAAGTACAACCTCGAAACCGGGTCGCACACGGTCTACGCGCTCCAATATCACTTCGTGACC
GTCACGAAGTACCGCGCAGACCTCCTCACCGACGAAGTCGCAGAACGGGTCGGTGAGATTGCTAGCGACATCTCAGAGGACTTCGGCGTGAACATTCAGA
ATGTCAACGGCGGTTCCGACCACGTTCATATCTTGTTCACGGCGAAGCCAACGACCGACCTCACCAAGTTTATCAACTCACTCAAGGGCGTTACGTCCCG
CAAAATCCGTGACGAGAATCCCGAGATTCGGCAGGCGCTCGACAAGGCGTTCTGGCAACCGGGGTACTTCCTCGCCACGACCGGCCAAGTGAGCATCGAC
GTGCTGATGGAGTACGTGGAGGAGCAGTAGCGATGTCCACGACCGCCACGAAGACGTTGCAGGCGACGTTCGCGCCACCGACGGCGCACAAGCAGTCGAA
ACTCAACGACCTGCTCGAAACGTATCGTGACGGTCTGCAAGAGGCGTTCGACTCCGGGGCGAGTACCATGTCGGCGGTGAGCGACATCGTGACGCCCTAC
GACCTGCCGTATCAGGCCAAAGCCGCCCTCTGCAACTACGTCCCGAAACTTCGGAAGACGTACAATGCCAAAGAGTTGGACGACGGCCACCCGATACGGC
TCACGAACCAAGCCACGAAGTTCGACCACTCCGCCGAACGCGACTACGAGTTTACATGGTGGGTTCCGCGTCCCGGCCGGGGAACGAACTTCTGGATACC
GCTTCGCATCAACCCTGAACAGGAAGACCTCTGGCATGACCTCGTATCGGAGGATGCGAAGGCGGGCGAGATACGGCTTCAGAAACACCGGAAGAACTGG
GTACTGCACGTCACCGTCGAGTACCCGGTCGAAGAACCAGCGACGGACGGTGACGTCACGCACATCGGCTTAGACATCGGAGAAACCGCCCTCATCACGG
GCTGTGCCCTCAAGGACGGTTCTCCGACTGACCCGTTTGTGTGTAGCGGAAGCAGAGCGAAGCATCTCCGCAAAGAGATGCACACGACCCTGAAACGCCT
CCAAGAGCGTGACGCATCCGAGTGGCGTATCGAAGACCGATTCAACCACTACCAGAACGCGCTCACCGACATCGTGGAGAAGGCGTCTCGGCAAGCCGTC
GAGTACGCCAAGCAGTTTGAACACCCGGTGTTGGTGATGGAGGACTTGACGTACATCCGCGAACGTCTTGACTACGGGAAGTATATGAACCGTCGCCTTC
ACTCGTGGGCGTTCGCCCGACTGCAAGGGCGCATCGAGGACAAGGCGACGGAAGCAGGTATCCCGGTCGAGTACGTGAATCCGGCGTACACCTCGCAGAC
GTGCCACTTGTGCCACCGTATCGGTCGGCGGGACTCCCAAGCCGAGTTCCGGTGTCCGAACAACGACTGCCACGTGTCGACGTTTCAGGCCGACATCAAC
GCTGCCGCGAATATCGCACGACGGGTTGACCCGTGGGGAGAGAGCGTCCCGCTTGACAAGGCCGGACGCGATGACTCGCCTCGGGATGGGAGCGGTTGTG
ACACCGCCACGACTCACCGTGAGAAGAGCGTACCAGCGCAGATGACGCTCACGGCCTACGAAGAGTCGGAACCCTCTGCCAGCGACGACTGACTGGTATT
CCCCATGCGTGGGAAGCCTCGCCGTTTACGGCGAGGAGGATGTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
393 bp130 aa138530+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKYNLETGSHTVYALQYHFVTVTKYRADLLTDEVAERVGEIASDISEDFGVNIQNVNGGSDHVHILFTAKPTTDLTKFINSLKGVTSRKIRDENPEIRQA
LDKAFWQPGYFLATTGQVSIDVLMEYVEEQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1260 bp419 aa5331792+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MSTTATKTLQATFAPPTAHKQSKLNDLLETYRDGLQEAFDSGASTMSAVSDIVTPYDLPYQAKAALCNYVPKLRKTYNAKELDDGHPIRLTNQATKFDHS
AERDYEFTWWVPRPGRGTNFWIPLRINPEQEDLWHDLVSEDAKAGEIRLQKHRKNWVLHVTVEYPVEEPATDGDVTHIGLDIGETALITGCALKDGSPTD
PFVCSGSRAKHLRKEMHTTLKRLQERDASEWRIEDRFNHYQNALTDIVEKASRQAVEYAKQFEHPVLVMEDLTYIRERLDYGKYMNRRLHSWAFARLQGR
IEDKATEAGIPVEYVNPAYTSQTCHLCHRIGRRDSQAEFRCPNNDCHVSTFQADINAAANIARRVDPWGESVPLDKAGRDDSPRDGSGCDTATTHREKSV
PAQMTLTAYEESEPSASDD

 

Blast result :
Comments
ISHhu2 is 99% (TnpA : the transposase) aa and 97% (TbpB) aa similar to ISHal1.
References
1] F. Pfeiffer (2013) Direct submission
2] Jaakkola S.T., Pfeiffer F., Ravantti J.J., Guo Q., Liu Y., Chen X., Ma H., Yang C., Oksanen H.M., Bamford D.H. (2015) Environ Microbiol. doi: 10.1111/1462-2920.13130