ISNpe14
- Family IS5
- Group ISH1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP003372 | ND | Natrinema pellirubrum | Natrinema pellirubrum DSM 15624 Natrinema pellirubrum DSM 15624 plasmid pNATPE01 Natrinema pellirubrum DSM 15624 plasmid pNATPE02 |
DNA section
IS Length : 1131 bp
Ends
IR Length : 10
IRL : TGCTCTGTTGCAGCGTCGAATAGCACCAGTTTCAGCCTTGTTCTCGGCTG
IRR : TGCTCTGTTGAACGCAAGACGGCGGCAGTATTGAAACTTTGACATTCAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTGCTTTGG | AAATAACA | CCTTCTACGT | 8 |
| ATCTCTTTGC | TTCCGAGT | GCCTTCTCCA | 8 |
| CTACAGTAGC | GGTGATGT | CCTAACGATC | 8 |
| AAGTCCTTCC | AGCCAACA | GCAAACGCGC | 8 |
| AGCTGGTAGT | GGTCATGT | ACAATTCACT | 8 |
| AGCCCGTGCC | ACTCGAGT | TCGTCGAGCA | 8 |
| TCGTGAACGA | ACATACCT | CGATCGTTAC | 8 |
| CTCACGAGGG | ACTCGACA | TCTATAGCAA | 8 |
| CAGAGATACA | GCGAGAGT | GCCTCGGGGT | 8 |
| TCGTCTTTGG | AGGCGACT | TCATCGTGGG | 8 |
| ACTGGTTTGC | TGAAAAGT | ACAATTTCGG | 8 |
DNA sequence
TGCTCTGTTGCAGCGTCGAATAGCACCAGTTTCAGCCTTGTTCTCGGCTGATTTCCGTGAGGCTTGTGGGGAATTGGACGCCGTCTTGCGATATGAGGTC
GAAACGGCCGATCATACGGCAGTGTAAGAATCTTGCCAAACAGCACGTGGATAACCCTGACGAACCCGCTGCGCCGGACGGCGCCAGCGGGTTCGCCGAG
TGGACTCAGATCGCGTTTATCCTCTTGCATGCGGAACTCGACAAAGATTTCCGAGAGACTGAAGCATGGTTCAATGACTCCAGAGCCATCCGTGAAGAGC
TTAACATCGACAAATCGCCGGATCACACCACGCTCTGTCGATGGGAGCAACAGGTCGACATGCGTGAACTCCGCAGCCTGCTCCGCCGCAGTGCGGAGCA
GGCTGGCTGGTCGGGAACAGCAGCAATCGACGCCAGTGGCTTCCAGCGAGATCAAACCAGCTATCATTACCGGAATCGTGCTGGCTTCTCGTTTCACAAG
CTGAAGACAACGATTTTGGTCGATACAGAGTCACTGGCGATCAAAGACGTTCATTTCACGACGAAGCGCAAGTGGGACGGACACATCGGCTTGCAGGTCT
ATCGGCGCAACGCCGAAGACCTGCAAGAGTTCCTTGCAGATGCGAACTATTCGTGGTCAGATCTCAGAGAGGAGTGTCGCGCTGGCGCGACACGACCGCT
AATCAAACACAGGGAGCACAATGCGCTGAAGAAAGCGCATAACGCTCGGATGGACGAAGATCTGTACCATCAGCGGACACTGAGTGAGACTGCGTTCTCA
CTGCTGAAGGATGACGGTGAGAAGTTACGCTCTCGGAGCTGGCACGGCCAGTTCCGAGAGCTCACGCGCAAGTGTATCGTGCATAACCTATCGCAGGCGG
CGAGTTAGGGCTCGCCGCCTGCTCTCTTTCTCTGGACGTATCCGCCAGAGACATCGCCGTCATCCACGTACGGGTTCTCACAAAGTGACCTAATTCTGAT
GGTTGCAGAATCAACGAACTGTACTCCTCCCACATCATCCAGATCTCAAAACCATCAGTACAGGAGTTCTAACGCCACCTGCCTGAATGTCAAAGTTTCA
ATACTGCCGCCGTCTTGCGTTCAACAGAGCA
GAAACGGCCGATCATACGGCAGTGTAAGAATCTTGCCAAACAGCACGTGGATAACCCTGACGAACCCGCTGCGCCGGACGGCGCCAGCGGGTTCGCCGAG
TGGACTCAGATCGCGTTTATCCTCTTGCATGCGGAACTCGACAAAGATTTCCGAGAGACTGAAGCATGGTTCAATGACTCCAGAGCCATCCGTGAAGAGC
TTAACATCGACAAATCGCCGGATCACACCACGCTCTGTCGATGGGAGCAACAGGTCGACATGCGTGAACTCCGCAGCCTGCTCCGCCGCAGTGCGGAGCA
GGCTGGCTGGTCGGGAACAGCAGCAATCGACGCCAGTGGCTTCCAGCGAGATCAAACCAGCTATCATTACCGGAATCGTGCTGGCTTCTCGTTTCACAAG
CTGAAGACAACGATTTTGGTCGATACAGAGTCACTGGCGATCAAAGACGTTCATTTCACGACGAAGCGCAAGTGGGACGGACACATCGGCTTGCAGGTCT
ATCGGCGCAACGCCGAAGACCTGCAAGAGTTCCTTGCAGATGCGAACTATTCGTGGTCAGATCTCAGAGAGGAGTGTCGCGCTGGCGCGACACGACCGCT
AATCAAACACAGGGAGCACAATGCGCTGAAGAAAGCGCATAACGCTCGGATGGACGAAGATCTGTACCATCAGCGGACACTGAGTGAGACTGCGTTCTCA
CTGCTGAAGGATGACGGTGAGAAGTTACGCTCTCGGAGCTGGCACGGCCAGTTCCGAGAGCTCACGCGCAAGTGTATCGTGCATAACCTATCGCAGGCGG
CGAGTTAGGGCTCGCCGCCTGCTCTCTTTCTCTGGACGTATCCGCCAGAGACATCGCCGTCATCCACGTACGGGTTCTCACAAAGTGACCTAATTCTGAT
GGTTGCAGAATCAACGAACTGTACTCCTCCCACATCATCCAGATCTCAAAACCATCAGTACAGGAGTTCTAACGCCACCTGCCTGAATGTCAAAGTTTCA
ATACTGCCGCCGTCTTGCGTTCAACAGAGCA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 816 bp | 271 aa | 93 | 908 | + | No |
Chemistry : DDE
ORF sequence :
MRSKRPIIRQCKNLAKQHVDNPDEPAAPDGASGFAEWTQIAFILLHAELDKDFRETEAWFNDSRAIREELNIDKSPDHTTLCRWEQQVDMRELRSLLRRS
AEQAGWSGTAAIDASGFQRDQTSYHYRNRAGFSFHKLKTTILVDTESLAIKDVHFTTKRKWDGHIGLQVYRRNAEDLQEFLADANYSWSDLREECRAGAT
RPLIKHREHNALKKAHNARMDEDLYHQRTLSETAFSLLKDDGEKLRSRSWHGQFRELTRKCIVHNLSQAAS
AEQAGWSGTAAIDASGFQRDQTSYHYRNRAGFSFHKLKTTILVDTESLAIKDVHFTTKRKWDGHIGLQVYRRNAEDLQEFLADANYSWSDLREECRAGAT
RPLIKHREHNALKKAHNARMDEDLYHQRTLSETAFSLLKDDGEKLRSRSWHGQFRELTRKCIVHNLSQAAS
Blast result :
Comments
ISNpe14 is 80% aa similar to ISNamo1.
References
1] Pfeiffer, F. (2013) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank