ISfinder Mirror Site

ISNpe10

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003373	ND	Natrinema pellirubrum	Natrinema pellirubrum DSM 15624 plasmid pNATPE01

DNA section

IS Length : 1835 bp

Ends

Left end : AAAGATACGAGCGCATACCCCCGTCTTTAGGCGGGGGTCAAGCGGACAATAGCCTACACACCCACCGACAACGCCACGGCTGGGTTTCCCACCGTTCTAT II struct. : Yes
Right end : AGAGACGCTTGGGACGTATGCGTCTTGAAACCATAGGGCCACGACCGGCCTGAAATCCCGTGGCGGGATTCCCGCGTCTTTAGGCGCGGGAGGAGGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TTCCTGCCGA	TGAT	TGAGTGGGTT	TCAA

DNA sequence

AAAGATACGAGCGCATACCCCCGTCTTTAGGCGGGGGTCAAGCGGACAATAGCCTACACACCCACCGACAACGCCACGGCTGGGTTTCCCACCGTTCTAT
CGGTGGTATTAAGACGACGACGGACCATAGTGTGCAACACGGATGAAGACCACACGGCACGCAACCTACAACCTCAACTACCACATAGTGTGGTTGCCGA
AGTACCGTCAATTGGGGCTCGTTAATGAGGTTGCCAGCCGTATCCGGTCCATCCTTCACGAAATAGCCGACGACAAAGGCGTCGAAATACTCGACCTCAC
CGTACAGCCCGACCACGTTCACCTGTTCGTCAGTAGCCCGCCCAAGAACGAACCGGCGCTACTCGCCAACTGGTTCAAGGGTATCTCCTCGCGCAAGTAC
AACCACCGCTACGCCGACCACGACGGCGAGAAAATCGGATGGGCGCGAGGATACTACGCAGGGACCGCCGGGCACGTTTCGAGTGAGACTGTCGAGAACT
ACATCCAACAGCACAAGGAGGGCGATTCGTGACCGAACTCACGAAGACGCTGGAACTCAACTTGTGGACCCGAACGCCCACAAGCGGAGGAAACTCCGAG
AGACGCGAGAGGCGTACCAGCACGCCCTCCTAGACGCATTCGACGCCCGATGTACCACGCAGACCGAAGCGAACGACGTGGTGGTCAACTACGACCTGTC
GGGGTACGCGAAGAACGCCCTCAAGAAGTACGTCCCGCAGTTGACGACGACGTACAACGCGGGCGGCCTTCACGACGACCACCCTGTTCGGTTCACCAAC
GAGGGGCTACGCCTCGACCACCAGCCCGAGAACGCTATCGAGTGGTACGTCAAAATCCCGCACCACGAAGACTACCACCTCTGGATGCCAGCACAGCCGA
ATCCCGACCAACGAGACTGGCTGGAAGCGTTGAACGCGGGAGACGCCACGATGGGCGAGAGTCGGCTGTTCCAGCGGGACGGGACGTGGTATCTCCACGT
CACCGCCACCCGCGACGTGGAGGAACGCTCCGAGGTGTCCGCCGAAGAACGGACGCCTATCGGAGTGGACATAGGGGAAGCGTCACTCGTCACGGTGTGT
CACCGCGATGACCACGGTTCTCCGACCACACCCGAACTGTGGGCCGACGAGGGCAAAACCGTTCGTCGGCTCCGCAAGACCTACTTCACCGCCACGCGCC
GACTCCAGACGCGAGGAAGCGAGCATATCGCGGAGTCCTTCGGGGGCGATATATGGAACCAGATAGACGACGTGTTCCACCGTGTCACCCGCGAAGTCGT
GGAGTACGCCGAGTCCGTCGAGAATCCTGTTCTAGTTCTGGAAGACCTGACGTACATACGGGAGTCGATGGACTACGGCGACTTTATGAACCGGCGTCTC
CACGGATGGGGATTCGCCAAACTCCACGCGCAGATACGGTACAAGGCCGTCGAGAAGGGTATCTCCGTAGAGACAGTGAACCCGCGCAACACGTCGAAGG
AGTGCCACGCTTGCGGAGAGGTAGGATACCGCCTGCGACAGGCGACGTTCAAATGTACGAACGACGACTGCTGGATGGGCGAGTACCAAGCGGACGTGAA
CGGTGCGATAAATATCGCAGACCGCTACCTCAGCGGAGAGAGTCATTCAAGAGAACACACGGATGGCGATGACTCGGCTGAGGATGGGGGGCGTTTGACC
GCCCCACAAGACACCCAAGCCGATGCTACCAAGCAAGAGACGCTTGGGACGTATGCGTCTTGAAACCATAGGGCCACGACCGGCCTGAAATCCCGTGGCG
GGATTCCCGCGTCTTTAGGCGCGGGAGGAGGTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
390 bp	129 aa	143	532	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKTTRHATYNLNYHIVWLPKYRQLGLVNEVASRIRSILHEIADDKGVEILDLTVQPDHVHLFVSSPPKNEPALLANWFKGISSRKYNHRYADHDGEKIGW
ARGYYAGTAGHVSSETVENYIQQHKEGDS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1200 bp	399 aa	564	1763	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MDPNAHKRRKLRETREAYQHALLDAFDARCTTQTEANDVVVNYDLSGYAKNALKKYVPQLTTTYNAGGLHDDHPVRFTNEGLRLDHQPENAIEWYVKIPH
HEDYHLWMPAQPNPDQRDWLEALNAGDATMGESRLFQRDGTWYLHVTATRDVEERSEVSAEERTPIGVDIGEASLVTVCHRDDHGSPTTPELWADEGKTV
RRLRKTYFTATRRLQTRGSEHIAESFGGDIWNQIDDVFHRVTREVVEYAESVENPVLVLEDLTYIRESMDYGDFMNRRLHGWGFAKLHAQIRYKAVEKGI
SVETVNPRNTSKECHACGEVGYRLRQATFKCTNDDCWMGEYQADVNGAINIADRYLSGESHSREHTDGDDSAEDGGRLTAPQDTQADATKQETLGTYAS

Blast result :

Comments

ISNpe10 is 65% aa (ORFA : the transposase) similar to ISSto1.

References

1] F. Pfeiffer (2013) Direct submission.
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank.