ISAot1

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_JH992958 ND Alloiococcus otitis
Alloiococcus otitis ATCC 51267
DNA section
IS Length : 1635 bp

Ends


IR Length : 17/23

IRL : GTTAGTGTAAAATTTATCTCGGTGGGGGGTTCCGGAATTCTGGACAAAAA
IRR : GTATGTGTCAAGTTTTCCTCGGTAACGTCAGAAACGCTCTATCAAAGTTC

Insertion site


Left flankDirect repeatRight flankDR Length
ATTTATGTGAGCGCTCTCATAAATACTGC9
CAACCGGGGCg/cAGTCCATTTCCCTCAAAGC12

DNA sequence

GTTAGTGTAAAATTTATCTCGGTGGGGGGTTCCGGAATTCTGGACAAAAAGAAAAGAGACCTCTATTCTTAAGTTAGGACACCAAAAGAAAGAGGTCTCT
TTATGAAGTCGATTATAACACAAATGCTTCAAATGGTAAAAGCAAACGCCAACCCGCTCGCTTGGGAAGAGGCGTTGCAGTATCTTTTTCAGGATTGGCG
GGAGGAGCTCGTCCTTCAAGCCTTGGGAGCCCTCGATAAGGCCTTATATTGGCATTATAAACAAGAGGGCTGGCGGATCGACCGCCTGGAACGGCGGACG
GTCCAGTTTACCTTTGGCAAGTTAACCTTTCAACGACGTCGCCTCCGCAAGAAAGGGGAAAAGAGTTTTTTACCCCTCGATCAAGCGATTGGGCTCGAAA
AACGCAAGCGCTACTCTTTAGCCGTCCGCAAAGGGATGGCGGAACTCGGTGCCCGGTTGCCCTTTCGGCAAGCCAGTCAGGTTGTGCGATTGTTAACCCC
GCTCACCATCAGCCATACAACCCTCCATACCATGACACAAGAAATCGGAGAACATCTTCAAAATTACAGCGAACACCGGCCTGAACCAGACGCATCAGCG
AAGCGAAAAGAAGTGCCAGTCTTATTTATTGAGGGGGACGGCTTATGTTTAAAAAGTCGAGATGGGGAGCCACTGACCTTGCATCGCCTGTTAATCCATG
AAGGCATTGACCGCCGTAAAAAACGAACGCGGTTGATTCACCCCTTTTGCTTTGTCAGTACCCAGTCGAGTGCCGAGGCCTTCCGGCAATTGAGTCATTA
CCTGAATCATCATTATCAGTTGAAACAGACCTTAGTGATTTCCAATAGTGATGGGGGGAGTGGCTATGAAGCGGACAAATTTGAAATGGCCATCGGTGCT
TGCCGGCAACATGAACATTGTCGAGACCCTTACCATGTCAACCGGAAGATCAAAGACCGCTTAAGCTTTGATCGACCGATGGAATACCAATTAAGAAAAG
CCTTGAGGGCTTATGACTGGACTCAAATCGAATCCGTTTTAGCCACCGCCCAAAGTCGTGTAGCAGCTTGCCCGTCGGAGATCGTTGCAGATCGACTGGA
CCAAATCGAGCGTTTGAGGGGCTATTTAAGTCGGCATTGGCCTTACCTGCAACCGATCGCGGACCGTTCTCTTCCCGTTCAAAAAGGAACGGGTGTCTGC
GAAACAGGGCACCGCTATTATAGCTATCGTCTCAAGCACCAAGGACGGGCTTGGACTCATGATGGTGCCCGTCATGTGGCTGCCATCCTCAATGCGGTGA
AAAACAAGGAATACGCCCGTATCTTAACGCAAAAAGGCGATCCCACCGTCCAATCACTGGGCGAAGACATCAAAGGAGCTGTCAAAGCGGCCCTTCGCAC
CCTGCAGCCAGCGACTAGCCAAGTCCAACAAGGGCATATTGTCAATCAAGGGCCGAGCTCCAGTCCTATAGGAAAACTGGCCAAAATGTTTCGCTAACAG
CCCCTGTCTCCAGGCCCCATGCGGGGAGCATGGGCCTGGAGACAAGGCATCATGCGCTAGCATGAGGAAAAACATTGGATAAAACGAACTTTGATAGAGC
GTTTCTGACGTTACCGAGGAAAACTTGACACATAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1395 bp464 aa1031497+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSIITQMLQMVKANANPLAWEEALQYLFQDWREELVLQALGALDKALYWHYKQEGWRIDRLERRTVQFTFGKLTFQRRRLRKKGEKSFLPLDQAIGLEK
RKRYSLAVRKGMAELGARLPFRQASQVVRLLTPLTISHTTLHTMTQEIGEHLQNYSEHRPEPDASAKRKEVPVLFIEGDGLCLKSRDGEPLTLHRLLIHE
GIDRRKKRTRLIHPFCFVSTQSSAEAFRQLSHYLNHHYQLKQTLVISNSDGGSGYEADKFEMAIGACRQHEHCRDPYHVNRKIKDRLSFDRPMEYQLRKA
LRAYDWTQIESVLATAQSRVAACPSEIVADRLDQIERLRGYLSRHWPYLQPIADRSLPVQKGTGVCETGHRYYSYRLKHQGRAWTHDGARHVAAILNAVK
NKEYARILTQKGDPTVQSLGEDIKGAVKAALRTLQPATSQVQQGHIVNQGPSSSPIGKLAKMFR

 

Blast result :
Comments
ISAot1 is 56% aa similar to ISTeha1.
References
1] ISfinder annotation (2013)