ISThio1
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_JH603164 | ND | Thiorhodovibrio sp. | Thiorhodovibrio sp. 970 |
DNA section
IS Length : 1756 bp
Ends
IR Length : 27/31
IRL : GGGTGCGGCTCCTTTAGAACGACGCTCAGCGACTAGAAAACAACAGCTTA
IRR : GGGTGCGGCTCTTTTGGAACGACGCCAAGCGCCGCAGAATCCAGCGGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAAGACTCC | ATCGACATC | GGTGATCAGC | 9 |
| GTCAAGACTCC | ATCGACATC | GGTGATCAGCA | 9 |
| AACAGTTACCA | AAACAAATT | CCCACGTGTTG | 9 |
| TGCCCGGCCTG | GATGGCTTT | GAGGTCTGCAC | 9 |
| CTTCTCCATC | AAATTCATTG | 0 |
DNA sequence
GGGTGCGGCTCCTTTAGAACGACGCTCAGCGACTAGAAAACAACAGCTTAACCAGCTAGAAACCTGAGGACAGGGAAGACAGGGCCTCCACTCGGGTGCG
AAACTGAAGGGACGAAACTTTTGTCACGGACCCTGAGGAGACCCTGTCGATGCCATGCTACACGATTTCTTTGCCAAGCCTCGATTGCTACGAGGGGGCG
ATCGCGCAGTTCGTCCAACTGCTTGAGACGCTGAGCGGGGAGCAAGCCCAACATGCCACGCACGGGGAAATCGAAGCGCTGGTGCAACAGGGCGGCATGA
AGCTGCTGTGTGAGATGGTCCAGTCGCACCTGGAACAAAGAGCGCGAGAGGAACCGCGGTATGCGTCCGTGATTGGCGCCGATGGTTATCCCCGGACCCA
TCATCGCGCCGGCTGTACCCGACTTCTGGAGACCCGCTTTGGGGAAGTGACCGTCACGCGCCGGGGCTACGGTGCGCGGGGACTCCAGAGCGTCTTCCCC
TTGGATGCTGAGCTGAATCTGCCCCCGGGCAGGTATTCCCATGGCCTGTGCGAGGTGCTGGCCGGTGAGGTCGTGAACAACTCCTTCGACGCCGCGCTTG
CGGCACTCGAACAAGCCGGCGGCGGGACGCTGGCCAAGCGCCAAGCCGAGGAGGTGGCCGTGCATCTGAGCCAGGACTTCGACGCCTTCTACGCCCAACC
CTTTGTCCCCCAGGAGCCGAATGAAGCGAGCGAACGCCTCCTGATCATCAGTGCTGACGGCAAGGGCATCGTGATGCGCCCCAGCGATTTGCGCGAGGCC
ACCCGCAAGGCGCGCGAGCGCCAAGCGCACAAGCAACAGACCCGGCTGAGTCCTGGGGAGAAAAAGCAGCGCAAACGCATGGCCACCGTCGCCTCGGTCT
ATGAGGTCGAGCCCTACCCACGCACCCCAGAGCAGATCCTTGATCCCAAACAAGCCCCCGCGGGCAAACGTCCCGAAGTCCAGAACAAACGCACCTGGGC
ACGCGTGGAGGCCGATCAGCGCACCGTCATCGAGCAGGCGTTTGAGGAGGCCATGCGCCGCGACCCGGATCAGCACCGCCGCTGGGTGGTGCTCTGTGAC
GGTCAGGAAGATCTGCTGCGCCAAGTCACAGCCGCGGCCGAGCGCTACAAGGTCGAGGTGGTGGTGATCCAGGACTTCATCCATGTGCTGGAATATCTGT
GGAAAGCCGCGCATGCGCTCTTTCCCGAGACCCCCGCGGAGCGCGAGCAGTGGGTGATGGAGCGCGCCATGGCCATCCTCGAAGGTCGCGCCCACGACGT
GGCCGTGGGACTGCGCCGCGCGGCAACGCGCAAGCAACTCGGCGAGACGGCGCGCAAACCCATCGATAAGGCGGCCGACTATATCGACAACAACCGCGAG
CGCCTCGAATACGACCAAGCGCTTGCGCTGGGGCTGCCAATCGCCACTGGTGTGATCGAAGGCGCCTGTCGCCATCTCGTCAAAGATCGCATGGATCTCA
CCGGGGCGCGTTGGGGGCTGGCCCGCGCCGAGGCGATCCTGAAACTGCGCTCACTAAAGATCAGTGGGGACTTGCCGGCCTATCTTGCGTTTCACTTCGA
CGCTGAGCACCGACGCCACTATCCAGGACCACCGATCCCGCTGGACTTGCCCGTGGCGGCATGATGTCTCACTTGCGCCATCACCATCGACCGGAAACGT
CGCCCGCAACCGCTGGATTCTGCGGCGCTTGGCGTCGTTCCAAAAGAGCCGCACCC
AAACTGAAGGGACGAAACTTTTGTCACGGACCCTGAGGAGACCCTGTCGATGCCATGCTACACGATTTCTTTGCCAAGCCTCGATTGCTACGAGGGGGCG
ATCGCGCAGTTCGTCCAACTGCTTGAGACGCTGAGCGGGGAGCAAGCCCAACATGCCACGCACGGGGAAATCGAAGCGCTGGTGCAACAGGGCGGCATGA
AGCTGCTGTGTGAGATGGTCCAGTCGCACCTGGAACAAAGAGCGCGAGAGGAACCGCGGTATGCGTCCGTGATTGGCGCCGATGGTTATCCCCGGACCCA
TCATCGCGCCGGCTGTACCCGACTTCTGGAGACCCGCTTTGGGGAAGTGACCGTCACGCGCCGGGGCTACGGTGCGCGGGGACTCCAGAGCGTCTTCCCC
TTGGATGCTGAGCTGAATCTGCCCCCGGGCAGGTATTCCCATGGCCTGTGCGAGGTGCTGGCCGGTGAGGTCGTGAACAACTCCTTCGACGCCGCGCTTG
CGGCACTCGAACAAGCCGGCGGCGGGACGCTGGCCAAGCGCCAAGCCGAGGAGGTGGCCGTGCATCTGAGCCAGGACTTCGACGCCTTCTACGCCCAACC
CTTTGTCCCCCAGGAGCCGAATGAAGCGAGCGAACGCCTCCTGATCATCAGTGCTGACGGCAAGGGCATCGTGATGCGCCCCAGCGATTTGCGCGAGGCC
ACCCGCAAGGCGCGCGAGCGCCAAGCGCACAAGCAACAGACCCGGCTGAGTCCTGGGGAGAAAAAGCAGCGCAAACGCATGGCCACCGTCGCCTCGGTCT
ATGAGGTCGAGCCCTACCCACGCACCCCAGAGCAGATCCTTGATCCCAAACAAGCCCCCGCGGGCAAACGTCCCGAAGTCCAGAACAAACGCACCTGGGC
ACGCGTGGAGGCCGATCAGCGCACCGTCATCGAGCAGGCGTTTGAGGAGGCCATGCGCCGCGACCCGGATCAGCACCGCCGCTGGGTGGTGCTCTGTGAC
GGTCAGGAAGATCTGCTGCGCCAAGTCACAGCCGCGGCCGAGCGCTACAAGGTCGAGGTGGTGGTGATCCAGGACTTCATCCATGTGCTGGAATATCTGT
GGAAAGCCGCGCATGCGCTCTTTCCCGAGACCCCCGCGGAGCGCGAGCAGTGGGTGATGGAGCGCGCCATGGCCATCCTCGAAGGTCGCGCCCACGACGT
GGCCGTGGGACTGCGCCGCGCGGCAACGCGCAAGCAACTCGGCGAGACGGCGCGCAAACCCATCGATAAGGCGGCCGACTATATCGACAACAACCGCGAG
CGCCTCGAATACGACCAAGCGCTTGCGCTGGGGCTGCCAATCGCCACTGGTGTGATCGAAGGCGCCTGTCGCCATCTCGTCAAAGATCGCATGGATCTCA
CCGGGGCGCGTTGGGGGCTGGCCCGCGCCGAGGCGATCCTGAAACTGCGCTCACTAAAGATCAGTGGGGACTTGCCGGCCTATCTTGCGTTTCACTTCGA
CGCTGAGCACCGACGCCACTATCCAGGACCACCGATCCCGCTGGACTTGCCCGTGGCGGCATGATGTCTCACTTGCGCCATCACCATCGACCGGAAACGT
CGCCCGCAACCGCTGGATTCTGCGGCGCTTGGCGTCGTTCCAAAAGAGCCGCACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1515 bp | 504 aa | 150 | 1664 | + | No |
Chemistry : DDE
ORF sequence :
MPCYTISLPSLDCYEGAIAQFVQLLETLSGEQAQHATHGEIEALVQQGGMKLLCEMVQSHLEQRAREEPRYASVIGADGYPRTHHRAGCTRLLETRFGEV
TVTRRGYGARGLQSVFPLDAELNLPPGRYSHGLCEVLAGEVVNNSFDAALAALEQAGGGTLAKRQAEEVAVHLSQDFDAFYAQPFVPQEPNEASERLLII
SADGKGIVMRPSDLREATRKARERQAHKQQTRLSPGEKKQRKRMATVASVYEVEPYPRTPEQILDPKQAPAGKRPEVQNKRTWARVEADQRTVIEQAFEE
AMRRDPDQHRRWVVLCDGQEDLLRQVTAAAERYKVEVVVIQDFIHVLEYLWKAAHALFPETPAEREQWVMERAMAILEGRAHDVAVGLRRAATRKQLGET
ARKPIDKAADYIDNNRERLEYDQALALGLPIATGVIEGACRHLVKDRMDLTGARWGLARAEAILKLRSLKISGDLPAYLAFHFDAEHRRHYPGPPIPLDL
PVAA
TVTRRGYGARGLQSVFPLDAELNLPPGRYSHGLCEVLAGEVVNNSFDAALAALEQAGGGTLAKRQAEEVAVHLSQDFDAFYAQPFVPQEPNEASERLLII
SADGKGIVMRPSDLREATRKARERQAHKQQTRLSPGEKKQRKRMATVASVYEVEPYPRTPEQILDPKQAPAGKRPEVQNKRTWARVEADQRTVIEQAFEE
AMRRDPDQHRRWVVLCDGQEDLLRQVTAAAERYKVEVVVIQDFIHVLEYLWKAAHALFPETPAEREQWVMERAMAILEGRAHDVAVGLRRAATRKQLGET
ARKPIDKAADYIDNNRERLEYDQALALGLPIATGVIEGACRHLVKDRMDLTGARWGLARAEAILKLRSLKISGDLPAYLAFHFDAEHRRHYPGPPIPLDL
PVAA
Blast result :
Comments
ISThio1 is 59% aa similar to ISFsp17.
References
1] ISfinder annotation (2013)
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Zhang,X., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Vogl,K., Liu,Z., Overmann,J., Frigaard,N.-U., Bryant,D. and Woyke,T. (2011) Direct submission GenBank.
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Zhang,X., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Vogl,K., Liu,Z., Overmann,J., Frigaard,N.-U., Bryant,D. and Woyke,T. (2011) Direct submission GenBank.