ISAcce1

  • Family ISKra4
  • Group ISKra4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_JH556659 ND Acetivibrio cellulolyticus
Acetivibrio cellulolyticus CD2
DNA section
IS Length : 1967 bp

Ends


IR Length : 24/31

IRL : GGGTGCATTTAATTTCCGCGAACCATATTGTGGAAATAACATCAATAAGT
IRR : GGGTGCATAAAATAATCGTGAACCATATTTTTACATTCAGGCTGCTTTTG

Insertion site


Left flankDirect repeatRight flankDR Length
TTTATTGGCACT/CCTTTCAATGCCATATTT11

DNA sequence

GGGTGCATTTAATTTCCGCGAACCATATTGTGGAAATAACATCAATAAGTTTTAGTGTTGTTTGATTACCCAAGAAGATTATTTTAAATCATAACCATTT
ATAAAAGTATAATAGCTAAATCTTGATATTATGGAGCTGATTTAGATCCAGTGCCTGTTGTTTTAGTGTCAAACGACTCCATGCGTGACCTTCGCAAAAA
ATCAGTAGGCCTCGGCTTGATAATTTTTCGCCGCGTCTATGGGACATCCTGTCCCGAGACGCTAAAAATGCCGTCCTTGGCATTTTTGCTTCAAAATTAT
CAAGCTTTCGGCCACTGATTTTAATGCTCAGGTCAAGCATTACGTCATTTGACACTAAAACAGGGTATAAGCCTAATTTCGGCCTTTCTTATGGATACAT
TTAGTTTTCGTACACTATATTATTCCAATTAGAAATTAGTAGTAGAAACACAGTTAAAAAATATAGAAAGCAAAAGGAATAGAAGCTGGAATAGTTCAAA
ACAATGGGCTTATTCCAGTTTTTGTTTTGCGTCATGATGTAAAATAGAGCATAAATTTACATATATAAACAAATATAAAAATCAATGTATAATAGGGGGT
TACAGAAGATGAACAATAAAATAGATGTAAATTCAATTATGAAAGAACTCGAAGAAAAATTAAGAAACGGTCTGAAAGATAATAAATTAGATGCTACAGG
TATTAGTCAATTGATTGGGGAACATCTGGAAAAAGCAAAAGAAAAGGTTTTACAAGATACAAGTAAAATGATAACTGAAGAAGTTAAGCCATCCGAAAAC
AAAAAGAAAAAGCAACCGTTAGTTATTTTAACGCCCTATGGTAAGCTTGTTTTTGTTCGTAATATGGACTATTGCAGTAAATGTGGGAAATTATACGGGC
ATAATGATGAATTATTCCAAATTAATGAGGAACATAGATTAACCAAAGATTCTCTTGAACTTATAACATATATAGGACAAATAATCCCCGGGTTTAATAA
TGCTCAAGAAGTATTATTAAAATTAAGGGGAATAGAAATCAGTGCTACCCAAATCCAAATTTTATCTGAAGAAGTTGGTAAAGAATTGTTTGAAATACAA
ATGGAAAAGTCTAATCTAGCATATTCCTGTCCTGAAGTAGCAGCTCCAGCAATGCTTGAAAAAGATAAAGAAAATACAATTTTATACATATCTATGGATG
GCTCTGCGGTTAACACTCGTGTTGAAGATGAAAATGGAAGCACATGGAAGGAAATGAAACTTGGACTAACATTCCTAGACAAGGATGTAATCAAGCGAAA
AAATGGCAGTTCCATTATCACAAAAAAGGAATATATTACTTACATGGGCAGTGTAGCAGAATTTAAGAAAGTGCTCTTTGATTCTGCTGCAAGAGCTGGA
TATGGCAGAGTGAAAAAGGTAGTGATAATTGGAGATGGTGCACATTGGGTATGGAATATGTGTGAAGAGATGTTTCCTGATGCTGAATACATTCTAGATT
TTTATCATATGACAGAAAACGTTTATAGCTATGCGAAAGAGTTGCTTTCATGCATTGAAAAGAAGTATAAAAAATGGGCAGATACAATGATATACTATAT
AAAGACAGAACAATTCAAAAAGGCATTGAAAAAGGTTGCAAGAAGTACTATAAGTGTGGATAAGGCTAACAAAACAGTGAATCTTGAAGGTTATATCAAA
AATAACATTGATAAGATGCATTATCTTGAATATAAAAACAAAAATTATTACATTGGTAGTGGTATGATAGAAAGTGGTAACAAGACCATTGTTCAAAAGC
GTATGAAGCAGGCTGGCATGCGGTGGGGTACTGATGGAGCTCAGTATATGGCCGTGCTAAGAGCTAAACATGAAAGTAACCGTTGGGAAGAGGTTGAGAA
AGTTATATTTAAGAAATCAAAAGCAGCCTGAATGTAAAAATATGGTTCACGATTATTTTATGCACCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1323 bp440 aa6091931+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNNKIDVNSIMKELEEKLRNGLKDNKLDATGISQLIGEHLEKAKEKVLQDTSKMITEEVKPSENKKKKQPLVILTPYGKLVFVRNMDYCSKCGKLYGHND
ELFQINEEHRLTKDSLELITYIGQIIPGFNNAQEVLLKLRGIEISATQIQILSEEVGKELFEIQMEKSNLAYSCPEVAAPAMLEKDKENTILYISMDGSA
VNTRVEDENGSTWKEMKLGLTFLDKDVIKRKNGSSIITKKEYITYMGSVAEFKKVLFDSAARAGYGRVKKVVIIGDGAHWVWNMCEEMFPDAEYILDFYH
MTENVYSYAKELLSCIEKKYKKWADTMIYYIKTEQFKKALKKVARSTISVDKANKTVNLEGYIKNNIDKMHYLEYKNKNYYIGSGMIESGNKTIVQKRMK
QAGMRWGTDGAQYMAVLRAKHESNRWEEVEKVIFKKSKAA

 

Blast result :
Comments
ISAcce1 is 49% aa similar to ISPle2.
References
1] ISfinder annotation (2013)
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Held,B., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Chang,Y.-J., Jeffries,C., Mouttaki,H., He,Z., Zhou,J., Hemme,C.L. and Woyke,T. (2011) Direct submission GenBank.