ISfinder Mirror Site

ISPle2

Family ISKra4
Group ISKra4

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_019689	ND	Pleurocapsa sp.	Pleurocapsa sp. PCC 7327

DNA section

IS Length : 1892 bp

Ends

IR Length : 22/23

IRL : GGGTGAGCGAAAAAAGATGTGGGGAAGGGGAGTTGGTTAGCGTTTATTTT
IRR : GGGTGAGCGAAAAAAGGTGTGGGAGGAGAGGAAGTAGTTAGCGTATTATT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AACCTAACAAA		CTCAATAGGT	0

DNA sequence

GGGTGAGCGAAAAAAGATGTGGGGAAGGGGAGTTGGTTAGCGTTTATTTTACGATAACATAAGTAAGACCTTCGACTCTTTAAAACGAAAAATGAATAAC
TTCCAGTCAATTCTGTCCCAAATTAATGAAACACTCAATTCATTACCAGACTGGAATAATTTATCCGAGTTTGTCAGTGAATTTCATGATATCTGGCTAA
AATTGGGAAATTTTGTGCAACAACAATTAGTACAAGCGCGTATTGAAGAAAAAGAACGGTTATACTCTACTCCGAGAACCATCTGGTCGAAAAGCTACTA
TACACCATTAGGTGAAATAAGTATTAAGCGCAGAGTTTATCAAACATCTGATGGTTTGAAAGTAAAAGTTGATGAAGAGTTGGGATTGCCAAAAGATAAA
TGGTTGCCAATGGTTTTAGAATTAGCTTGTGCCTTGGGGATTAGTAGTGAATTCCCCAATTCTCATCAACTTTTTCCAAGATGGACTCATCTTGAATTAA
CGGAAAAAACCCTTGCCAATCAAGTTGAAAAGACAGGAAATATTCTACAATCACAAGAATTTAGTTTCGACCATCAACAAATGCCTAGTAGAGCGCCTGA
ATCTACAAATTCAGATTCATCTAAATCAGATTTATTATATGTGGGAGTTGATGGAGTCATGACTCCCCTTAATCAAAAACAGGGCTATAAAGAAGCCAAA
GTCGGTGTAATTTTTTGGAGTAAAGACCATCAAAAAATTAAGGGTAAAAGAGGGAAAATACGTCGAAGAGAATATGTCGCAACTTTAAAATCACGTAAGG
AATTTAAGAATAGAGTATTCCAATTATATAATCAACTAGCAGGTCAAAAAGCTACAAATACAGTAGTTATTGGTGATGGCGCACATTGGATTTGGTCAAT
GGCACAGGAACAATTTCCTGGTTCAATAGAAATTCTCGATTTTTTTCATCTCTCAGAATATGTTTGGGCAGTAGCTAAAGCAGCTTATCCAAAAGGGGAA
CAACAACAAAAAGATTGGGTGAAAATTCAACAGCAATTGCTGAAAAAATCTCAGTGGCAAACTGTTATTCACAATTCTCATAAATTAAAAAGAAACAAGA
AAGATTTAATCGAAGCGATTACTGACCTAGAGCGTTATTTAACTAATAATCAAAGTCGAATTGATTATCAATCCTATTTAAAAGCCGGGTTAATGATTGG
TTCAGGTGTAGTTGAAAGTTCCAATCGTCGTGTAGTTACTCAAAGATTAAAACAAGCAGGAATGCACTGGTCAGCTTTTGGAGCAGAAGGAGTTATGGCT
TTGAGGGCAGCTTATTTAAGTAATTCAAACCGTTGGTTAAATTTTTGGTCGCGAGAGAGTAGAATGTTTTTAACAAGTTAGCGTAATTTTCGGCATAACT
TTTGTCTCTTTTACTTCAAAAGTGGTCAAAACTCAACAACAGCAATTAGATTTAGCCAACAGAATCGCACAAGTACAAGCTTTAATTGATGAAATCTCAA
TAGAGATTGAGAAGATTCAAGCAAACTCCCCAGTGGCACCCCCAACTTGCTCCCTTATTCGTTTTCGTGCTAAAGGAAAGGGTGGAGCTTATTGGTATTA
CAAATGGCAATCTCCCCAACCAATATTCGTTACGAAAAAAGGTAATTATTCTCGTTACAAATATATTGGCAAAGCAGGTAGTCCCGCTTTTTTAGAAGCG
GTAGAAATGTATCGTAATCGTACTCAAATTTCTGCTCTAGAACAAGTACGACATACATTGGAATTGGGTCGAGAAGATTTATTAGAAGAAGCCAGCAGAA
CTCGTGACTAATTTTCAGTTTAATTCGGACTTTGTTATCGTAAATAATACGCTAACTACTTCCTCTCCTCCCACACCTTTTTTCGCTCACCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1290 bp	429 aa	92	1381	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNNFQSILSQINETLNSLPDWNNLSEFVSEFHDIWLKLGNFVQQQLVQARIEEKERLYSTPRTIWSKSYYTPLGEISIKRRVYQTSDGLKVKVDEELGLP
KDKWLPMVLELACALGISSEFPNSHQLFPRWTHLELTEKTLANQVEKTGNILQSQEFSFDHQQMPSRAPESTNSDSSKSDLLYVGVDGVMTPLNQKQGYK
EAKVGVIFWSKDHQKIKGKRGKIRRREYVATLKSRKEFKNRVFQLYNQLAGQKATNTVVIGDGAHWIWSMAQEQFPGSIEILDFFHLSEYVWAVAKAAYP
KGEQQQKDWVKIQQQLLKKSQWQTVIHNSHKLKRNKKDLIEAITDLERYLTNNQSRIDYQSYLKAGLMIGSGVVESSNRRVVTQRLKQAGMHWSAFGAEG
VMALRAAYLSNSNRWLNFWSRESRMFLTS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
390 bp	129 aa	1422	1811	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MVKTQQQQLDLANRIAQVQALIDEISIEIEKIQANSPVAPPTCSLIRFRAKGKGGAYWYYKWQSPQPIFVTKKGNYSRYKYIGKAGSPAFLEAVEMYRNR
TQISALEQVRHTLELGREDLLEEASRTRD

Blast result :

Comments

ISPle2 is 46% aa (ORFA : the transposase) similar to ISDesp4.
The second ORF is a passenger gene annotated as hypothetical protein.

References

1] ISfinder annotation (2013)
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C., Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and Kerfeld,C. (2012) Direct submission GenBank.