ISDau4
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012108 | ND | Desulfobacterium autotrophicum | Desulfobacterium autotrophicum HRM2 |
DNA section
IS Length : 1609 bp
Ends
IR Length : 19/22
IRL : GGGAGCATTAGGCGTTTCTTAACGGAATCTTATGAGAGACAGAGGAAAAC
IRR : GGGAGCATTACCTGTTTCTTAAGAATTATCCACTATGAGACGAAGTTGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCAACGAGG | TTTAAAAAG | TGAATTCCTA | 9 |
DNA sequence
GGGAGCATTAGGCGTTTCTTAACGGAATCTTATGAGAGACAGAGGAAAACTTGCTGAAAGGCTTGATATGTGATTATAATTCTTTGTATTTATAGATAAT
AAAGCTGGACAATTCCATAAGAATAGATTAACTTATCATGGGAAATAGCCCTCATTTTTTAAAGGAGGCCCCATGATAGCAACTGCTAAAATCGCAAGCG
CTTATTCAGAAGAATGGAAGTCAGGATTTCTATCATTTGCCAGTAGCCGTTTAGACAAAATAGCAAAGGATTACCAGATGGATTCCATCACCGATATATC
GAAAAGTCTTTTCAGCAACCGATCAGACATTATGGGGCAGGCCGCTTTAGCATTGATAGAAAAAAAATATGGTCACCTACTGGAGCAAAAACATTGTCAA
TGCCCGATTTGCGACAAACAAATAAAAGCAAGGCCCCAAAAAGTAAAACGGAAGATTGAAACACTTATAGGTCCAGTAGAGCTTGAACGGCCTTATTTTT
ATTGCGTCAAATGTGGTTTCGGATTCTATCCGCTGGATTATGCCTTGGGGCTTTCGAATCGAAAAACACAATCTGACATTCAAGAGTTGGAAGCCTGGTT
GGGGGCCGAAATGCCTTATGAAACAGCGGCTGAAACCCTTGAGCGATGTGCAGGAGTTAAGGTCAGTAACCACCATGCCCACGATGTTGTTAATGAAGTA
GCAGAGCATATTCAACTGTTGGATGTTTGTCCTGACAAATCAGAAATCCATGCTCAAATTGATGCCATCGCTAAGAATAATTTCCGCCGCCCCGTTTTAA
TGATAGGCATAGACGGAGCCCATGCACCAACACGCCCAGAGCCCAGTCCGAGAAAGGGTAAAAGAGGGAAAGGTGAATGGAGAGAGGTCAAGGGGTTTCG
GATTTATCTGATTGATAATCAAAACATTAATCACCTGATAAGCTGGCACCAAATCAAAACAGACAAAGAGTTGACACAGGATCTGTTGGAGATCAAGCAG
GCCGGGTTAATACCCGAAAAAAAAGTCCGCATATGTGTCATTGGAGATGGTGCTCCATGGATTTGGAATCGAATAAAGGAAGTTTATCCGAATTCTAAAA
AAGTATTGGATTATTATCATTGCAGCGAGTATTTGCATGATGTGGCCAATGCCCAATACGGCAAGAACACACAAAAAGCCAGAGAATGGGTGGAGGCAAC
GCTTACCAGACTTTTTTTAAACCAGGCAGATAGAATTATTGCCGGAATGAAGCGAATGAAACCAAATTCAGATTCAGCTATGGAACAAATTAACAAGACT
GTGAAATATCTTTCTGATCGTAAAGAAATGATAAATTACGGTTCATTGAAGAGAGGTGGTTATCATATTGGCAGTGGTGGTATAGAAAGCTCAAATAAGT
TCATATCCAATGTACGCCTTAAACGATCCGGTGCATGGTGGTATCCTACAAACGCCAACAATATTCTAAAGCTACGTTGTGCGAAGTATAATAAAACTTT
TGATCGAGTAATGGAAAGAAGTAGGAAAAAATTACAGCAAAGTAAAACCGAAAAGCGTCCTCAACTTCGTCTCATAGTGGATAATTCTTAAGAAACAGGT
AATGCTCCC
AAAGCTGGACAATTCCATAAGAATAGATTAACTTATCATGGGAAATAGCCCTCATTTTTTAAAGGAGGCCCCATGATAGCAACTGCTAAAATCGCAAGCG
CTTATTCAGAAGAATGGAAGTCAGGATTTCTATCATTTGCCAGTAGCCGTTTAGACAAAATAGCAAAGGATTACCAGATGGATTCCATCACCGATATATC
GAAAAGTCTTTTCAGCAACCGATCAGACATTATGGGGCAGGCCGCTTTAGCATTGATAGAAAAAAAATATGGTCACCTACTGGAGCAAAAACATTGTCAA
TGCCCGATTTGCGACAAACAAATAAAAGCAAGGCCCCAAAAAGTAAAACGGAAGATTGAAACACTTATAGGTCCAGTAGAGCTTGAACGGCCTTATTTTT
ATTGCGTCAAATGTGGTTTCGGATTCTATCCGCTGGATTATGCCTTGGGGCTTTCGAATCGAAAAACACAATCTGACATTCAAGAGTTGGAAGCCTGGTT
GGGGGCCGAAATGCCTTATGAAACAGCGGCTGAAACCCTTGAGCGATGTGCAGGAGTTAAGGTCAGTAACCACCATGCCCACGATGTTGTTAATGAAGTA
GCAGAGCATATTCAACTGTTGGATGTTTGTCCTGACAAATCAGAAATCCATGCTCAAATTGATGCCATCGCTAAGAATAATTTCCGCCGCCCCGTTTTAA
TGATAGGCATAGACGGAGCCCATGCACCAACACGCCCAGAGCCCAGTCCGAGAAAGGGTAAAAGAGGGAAAGGTGAATGGAGAGAGGTCAAGGGGTTTCG
GATTTATCTGATTGATAATCAAAACATTAATCACCTGATAAGCTGGCACCAAATCAAAACAGACAAAGAGTTGACACAGGATCTGTTGGAGATCAAGCAG
GCCGGGTTAATACCCGAAAAAAAAGTCCGCATATGTGTCATTGGAGATGGTGCTCCATGGATTTGGAATCGAATAAAGGAAGTTTATCCGAATTCTAAAA
AAGTATTGGATTATTATCATTGCAGCGAGTATTTGCATGATGTGGCCAATGCCCAATACGGCAAGAACACACAAAAAGCCAGAGAATGGGTGGAGGCAAC
GCTTACCAGACTTTTTTTAAACCAGGCAGATAGAATTATTGCCGGAATGAAGCGAATGAAACCAAATTCAGATTCAGCTATGGAACAAATTAACAAGACT
GTGAAATATCTTTCTGATCGTAAAGAAATGATAAATTACGGTTCATTGAAGAGAGGTGGTTATCATATTGGCAGTGGTGGTATAGAAAGCTCAAATAAGT
TCATATCCAATGTACGCCTTAAACGATCCGGTGCATGGTGGTATCCTACAAACGCCAACAATATTCTAAAGCTACGTTGTGCGAAGTATAATAAAACTTT
TGATCGAGTAATGGAAAGAAGTAGGAAAAAATTACAGCAAAGTAAAACCGAAAAGCGTCCTCAACTTCGTCTCATAGTGGATAATTCTTAAGAAACAGGT
AATGCTCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1314 bp | 437 aa | 278 | 1591 | + | No |
Chemistry : DDE
ORF sequence :
MDSITDISKSLFSNRSDIMGQAALALIEKKYGHLLEQKHCQCPICDKQIKARPQKVKRKIETLIGPVELERPYFYCVKCGFGFYPLDYALGLSNRKTQSD
IQELEAWLGAEMPYETAAETLERCAGVKVSNHHAHDVVNEVAEHIQLLDVCPDKSEIHAQIDAIAKNNFRRPVLMIGIDGAHAPTRPEPSPRKGKRGKGE
WREVKGFRIYLIDNQNINHLISWHQIKTDKELTQDLLEIKQAGLIPEKKVRICVIGDGAPWIWNRIKEVYPNSKKVLDYYHCSEYLHDVANAQYGKNTQK
AREWVEATLTRLFLNQADRIIAGMKRMKPNSDSAMEQINKTVKYLSDRKEMINYGSLKRGGYHIGSGGIESSNKFISNVRLKRSGAWWYPTNANNILKLR
CAKYNKTFDRVMERSRKKLQQSKTEKRPQLRLIVDNS
IQELEAWLGAEMPYETAAETLERCAGVKVSNHHAHDVVNEVAEHIQLLDVCPDKSEIHAQIDAIAKNNFRRPVLMIGIDGAHAPTRPEPSPRKGKRGKGE
WREVKGFRIYLIDNQNINHLISWHQIKTDKELTQDLLEIKQAGLIPEKKVRICVIGDGAPWIWNRIKEVYPNSKKVLDYYHCSEYLHDVANAQYGKNTQK
AREWVEATLTRLFLNQADRIIAGMKRMKPNSDSAMEQINKTVKYLSDRKEMINYGSLKRGGYHIGSGGIESSNKFISNVRLKRSGAWWYPTNANNILKLR
CAKYNKTFDRVMERSRKKLQQSKTEKRPQLRLIVDNS
Blast result :
Comments
ISDau4 is 62% aa similar to ISTni1.
References
1] ISfinder annotation (2013)
2] Strittmatter,A.W., Liesegang,H., Rabus,R., Decker,I., Amann,J., Andres,S., Henne,A., Fricke,W.F., Martinez-Arias,R., Bartels,D., Goesmann,A., Krause,L., Puhler,A., Klenk,H.P., Richter,M., Schuler,M., Glockner,F.O., Meyerdierks,A., Gottschalk,G. and Amann,R. (2009) Environ. Microbiol. 11 (5), 1038-1055.
2] Strittmatter,A.W., Liesegang,H., Rabus,R., Decker,I., Amann,J., Andres,S., Henne,A., Fricke,W.F., Martinez-Arias,R., Bartels,D., Goesmann,A., Krause,L., Puhler,A., Klenk,H.P., Richter,M., Schuler,M., Glockner,F.O., Meyerdierks,A., Gottschalk,G. and Amann,R. (2009) Environ. Microbiol. 11 (5), 1038-1055.