ISAcif1

  • Family ISNCY
  • Group ISMae2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_015942 ND Acidithiobacillus ferrivorans
Acidithiobacillus ferrivorans SS3
DNA section
IS Length : 2464 bp

Ends


IR Length : 17

IRL : CAGCAATTCTCAATATGGCCAAGAGCCCTTTTTTTTTGGGTAGCGTCGCC
IRR : CAGCAATTCTCAATATGAAGTTTGTCGGCCTCCCCGATCTCTCGGGACAA

Insertion site


Left flankDirect repeatRight flankDR Length
CTCCAGGTGCCTTATGTGGGGCCTGTGGA9

DNA sequence

CAGCAATTCTCAATATGGCCAAGAGCCCTTTTTTTTTGGGTAGCGTCGCCGTGTGAATGATTCGGTTTTTGAGGTGGACGGTGAGCGCTGGACAAATCTA
TCTGTTTAACGTATAAGAGTTTGGGTTAATGTCATTCCAGTGAGAACGTATGGCCTGTCCCGTTGTTTCTCCTCAGCAAGCCCTTCGCATGCCAGAGCAA
ATCGCCGTACTCAGAGCGTTGGCGCGTGACTTCCCAGACAACCGGACCGGCAAAAATTGCACTTATCGCATGGAAGATGCCTTGTTAGGCGCATTCTCGG
TGTTTTATACCCAATGCCCTTCTTTCCTGACACATCAAATCGCCATGAGCGAAACCCGGGGCATGAGTAATGCGCAAACCTTGTTTGGTATGCGCGACAT
CCCCAGCGACAACCATATTCGCAACCTGTTGGATCCTGTCGACCCACAGCATATTTATCCAGCCTTTGAGCGGACTTTCAATGCCCTGAATGATACCGGT
CAGCTGGACACCTTCCGGATGGTGAATGGACAGCTGTTGATCGCGCTGGATGGGACGAGTTATCACCAATCCCATGCCATTCATTGTCCGCAATGTACGG
TCACAGAGCATAAAAAAGGCGGAACCAGCTATACCCACACCGTCGTTACGCCGGTCGTCGTTTCCACCGCACACAACCGCGTTATCCCCCTGGAACCGGA
ATTCGTGACCCCGCAAGACGGCCATGCCAAGCAGGATTGCGAATCGGCCGCAGCGCATCGCTGGATTACCGCCTATGCCGAGCGCTATCGACACCTGCAT
GTGACCCTGCTGGGCGATGATCTGTATAGCCGCCAACCCATGTGCGAGGAGATGCTCAGAGCGGGCTTCGACTTCATCCTGGTCTGTAAACCCGCATCGC
ATACCACGCTTTATGCGGAACTCGAACGCCGGGAACATACGGGAAACGTATCTACTTTCAGCATGACTCGACGCGAAGGCAAGAAGACCTTCACGGACAC
CTACCGCTTCGCCGAATCGCTGCCGTTGCGGGCGGGTAAGGATGCACTGATGGTGAATTGGTGCGAGTTAAAGTCCACCAGCGCAGAAGGCAAGACGGTG
TATCACAACGCCTTTGCCACCACGCACACCCTCCATCCCAGTAATGTTGCTGAAATCGTAAAAGCGGGACGTGCGCGGTGGCGCATTGAAAACGGCAATA
ACAATACGCTGAAAACCAAGGGGTACAACCTGTCCCACAATTTCGGGCATGGTCAAAAGTATCTTTCAGCTACCTTGGCGACATTGAATCTGTTGGCTTT
TCTGCTGCATACCGTGCAAGAACTGACCGACTTCACCTATCGGACTTTGCGGGATAGACTCCCCACGCGAAAGGCCTTCTTTGAGCATGTCCGTGTTCTG
ACGCAGTATCATTGCTTCGAGAGTTTCGCGGCACTCCTGGAATTCATGCTGAGTGGGCTCAACCGGGGCCGCCGCGACAGTGGTTGAACGATGATAACGG
AAGCGCCGGATTACCATTTCTGCGGTCAGGCCTTGAGCGCTGCAGAGTGCCAACTCATTCAGGATCTCACGTATCAGTATGGCCGTCTGAGTCGCCATGT
TTTAGCGCAAACCATATGTGAACTGCTCGGCTGGCAGCGCCCTAACGGGAATCTGAAGGCGGCAGAGTGCCGGATACTGCTTGAACAGATGCAGGACGAA
GGATGGATCCACTTGCCTGCGCTACGCGCTGGACGACCACGGGGTCGCAGGACTGGCACCTCTGCCATGCCAGTGCCCCTCTCCGAGATGCCCACCGCAC
TCGCAGCACTGCAGCCGATTAAACTGCAACGCGTGGAAACACCGGCACAGCGCCAGTTATGGCGAGACCTGATCGCACAGCATCATTATCTGGGCCACAA
AGTCCCTTTTGGGGCGCATCTGCGTTATTTAATTCAGGGAATACCCCATCCAGACGCTCTGCATTCGCCCTGGGTATTAGGATGCTTACAGTTTTCGTCA
CCGGCTTGGCGAATGCGTGCCCGGGATGCCTGGATCGGCTGGGACGATGCGACCCGGGCCAAACGCCTGCAGCAGGTTATTTGCAACTCCCGATTTCTGA
TCCTACCGCAGGTACAGGTCAAGAATCTCGCCAGTCATGTATTGGCTCTGGCTCTGCGCACCGTAACCCGTGATTGGGAGGCCGTTTACGGATTACGTCC
TTATCTGGTAGAAACGCTGGTCGATCCAGTACGCTTTACCGGTCATTGTTACCGGGCAGCAAACTGGATTGATGTCGGACTCACCACCGGACGCGGTCGG
CAAGATCGTCAACACGCTCTTCATGATGCCCATCCAAAGCGCATCTTCCTCTATCCTTTAACTCCTAACGCGCAGCAAAAGCTGCGGCAAAGCACCTAAA
AACTGCTCGAAAATTTGTCCCGAGAGATCGGGGAGGCCGACAAACTTCATATTGAGAATTGCTG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1338 bp445 aa1501487+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MACPVVSPQQALRMPEQIAVLRALARDFPDNRTGKNCTYRMEDALLGAFSVFYTQCPSFLTHQIAMSETRGMSNAQTLFGMRDIPSDNHIRNLLDPVDPQ
HIYPAFERTFNALNDTGQLDTFRMVNGQLLIALDGTSYHQSHAIHCPQCTVTEHKKGGTSYTHTVVTPVVVSTAHNRVIPLEPEFVTPQDGHAKQDCESA
AAHRWITAYAERYRHLHVTLLGDDLYSRQPMCEEMLRAGFDFILVCKPASHTTLYAELERREHTGNVSTFSMTRREGKKTFTDTYRFAESLPLRAGKDAL
MVNWCELKSTSAEGKTVYHNAFATTHTLHPSNVAEIVKAGRARWRIENGNNNTLKTKGYNLSHNFGHGQKYLSATLATLNLLAFLLHTVQELTDFTYRTL
RDRLPTRKAFFEHVRVLTQYHCFESFAALLEFMLSGLNRGRRDSG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
909 bp302 aa14912399+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MITEAPDYHFCGQALSAAECQLIQDLTYQYGRLSRHVLAQTICELLGWQRPNGNLKAAECRILLEQMQDEGWIHLPALRAGRPRGRRTGTSAMPVPLSEM
PTALAALQPIKLQRVETPAQRQLWRDLIAQHHYLGHKVPFGAHLRYLIQGIPHPDALHSPWVLGCLQFSSPAWRMRARDAWIGWDDATRAKRLQQVICNS
RFLILPQVQVKNLASHVLALALRTVTRDWEAVYGLRPYLVETLVDPVRFTGHCYRAANWIDVGLTTGRGRQDRQHALHDAHPKRIFLYPLTPNAQQKLRQ
ST

 

Blast result :
Comments
ISAcif1 is 66% aa (ORFA : the transposase) similar to ISAtsp9.
The second ORF is a passenger gene annotated as hypothetical protein.
References
1] ISfinder annotation (2013)
2] Liljeqvist,M., Valdes,J., Holmes,D.S. and Dopson,M. (2011) J. Bacteriol. 193 (16), 4304-4305.