ISAcas3

  • Family ISKra4
  • Group ISKra4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AFEJ01000309 ND Acaryochloris sp.
Acaryochloris sp. CCMEE 5410
DNA section
IS Length : 1484 bp

Ends


IR Length : 29/31

IRL : GGGCGTAGCCACTAGTTGGGGGAGTCCAAATACTCTATAAGAAAGAAGGA
IRR : GGGCGTAGCCACTAGTTGGGGGAGTACAGATTGGGTCTGAGCGCTTCCTC

Insertion site


Left flankDirect repeatRight flankDR Length
TCTCTAGAAAACGTTGGACATTGCTCAGGA9

DNA sequence

GGGCGTAGCCACTAGTTGGGGGAGTCCAAATACTCTATAAGAAAGAAGGATGGCATCACACCATCCCTACCAAATAAAAAAGCTCCTTTGACTATAGATC
CCATCACCTCCCTTTGCCTAGAGCCTGAACAGTGGCAACGCTATCACCAACGCCTCAATGACTCCTCTAGCCTGAGTGCCATGGTTTGGGCAGCTCTGGC
CCTAGGTTTGTGGCTTGCCCGCAATCTTGTTGAACAAGCACTAGAGCACCGCGCCCAAGCCCACACTCAATGGCCTGATTGCCCCGGATGTGGCCGACGT
CTTCGGTCTAAAGGGTTCAGATCCAGACAGATGCTCACCTGCTTGGGGATGATCCATTGGCGTCGTCGCGTCGGGCGTTGCCCAGGGCATTGTCACGGCT
GCGAGGTAATTCCCTTGGATACTGCATTGGGCATCAGCGCCCATCAACGGACTAGTGTTGAGTTAGAGCGGCTCGGGTGTTTACTCAGCTTATTTGTTCC
CTTTGAGTTAGCCGCTGAGCTGCTCGGGCAAATCAGTGGGCATTCCCTCTGCTCGTCAACCTTGTGGAACTGGGTGCAACAGCGAGGTCAACAGGCCAAA
ACGCGACTAGAAGCTCAAGTCCAGGCCCTGAGGTTAGGGGAGGCAGTGATCCCTGAGGAGATGGAGGCATTCATTTGCCAGATGCTGATGGTGATTGGTG
CGGATGGGGTGATGGTGCCGATGCGCCCCAGCGGTGGTCAGCCCAAGGGTAAGATTGTGTGGCAGGAGGTCAAGGTGGCTGTGGTGGCCCGTTTAGGTGA
GCATTGGACGCGCACAGGCAACCGGGTCAAACGTCTGTATCAACGTCGGCTGGTAGCACGGTTGGGGACGATTGATGAGTTTGCACCGTGGTTGCAGATG
GAGGCGCTACGTCAAGGTTTACTGAGCGCTCCCAAAGTAGTGTGGATCAGTGATGGGGGCAAGGGCTTTTGGCGGCTCTATCATCAATACTTTGCCCGAT
ATGGACAGGGCATTCTCGATTTTTACCATGCGGCTGGGCAATTGTGGCGAGCCGCCGCCGCTTGGCTCGACGGTCGCACGGCTGATGCACGATGGTGCTT
CGAGCGATGGCGACATTTATTGCGTCATGGCGGGGAACAACGGCTGTTGACTGAGTTGACCCAACTGATCAATCGGGATGATCTCTCAGACCCTGCGCTG
GAGACGTTGATGCAAGTGCAGCGCTACCTACAATGCCATCGTCAGCACATTCGCTATCAACGCTGTGACAAGCAAGACTTTCCCCTCGGATCTGGCTTGG
TCGAAAGTGCTTGTAAGTGGCTGATTCAGCAACGATTTAAGGGGGTCGGGATGCGATGGAGTGAAGATGGACTCAACCACTTACTTCATCTGAGGCTTAA
CTGGGTTAATCATAGGTTTGATGAGCTATTTCCTGAGGAAGCGCTCAGACCCAATCTGTACTCCCCCAACTAGTGGCTACGCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1386 bp461 aa881473+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTIDPITSLCLEPEQWQRYHQRLNDSSSLSAMVWAALALGLWLARNLVEQALEHRAQAHTQWPDCPGCGRRLRSKGFRSRQMLTCLGMIHWRRRVGRCPG
HCHGCEVIPLDTALGISAHQRTSVELERLGCLLSLFVPFELAAELLGQISGHSLCSSTLWNWVQQRGQQAKTRLEAQVQALRLGEAVIPEEMEAFICQML
MVIGADGVMVPMRPSGGQPKGKIVWQEVKVAVVARLGEHWTRTGNRVKRLYQRRLVARLGTIDEFAPWLQMEALRQGLLSAPKVVWISDGGKGFWRLYHQ
YFARYGQGILDFYHAAGQLWRAAAAWLDGRTADARWCFERWRHLLRHGGEQRLLTELTQLINRDDLSDPALETLMQVQRYLQCHRQHIRYQRCDKQDFPL
GSGLVESACKWLIQQRFKGVGMRWSEDGLNHLLHLRLNWVNHRFDELFPEEALRPNLYSPN

 

Blast result :
Comments
ISAcas3 is 66% aa similar to ISLesp2.
References
1] ISfinder annotation (2013)
2] Miller,S.R., Wood,A.M., Blankenship,R.E., Kim,M. and Ferriera,S. (2011) Genome Biol Evol 3, 601-613.