ISGlsp1

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_ALVY01000084 ND Gloeocapsa sp.
Gloeocapsa sp. PCC 73106
DNA section
IS Length : 1200 bp

Ends


IR Length : 30/37

IRL : GGGAGCAAGTCAAGTTTGTTAGAACACCTGTACTATTGACAAAAAAATAG
IRR : GGGAGCAAGTCACAATTGCTAGAACAAATGTACTAGTCAAAGGCTATTAT

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNGCTAAAGGGCTTCTGGTAGG0

DNA sequence

GGGAGCAAGTCAAGTTTGTTAGAACACCTGTACTATTGACAAAAAAATAGATATGTGTAGAGTATAAAGTTAGCTTCAAACTTAACCAGGAAATATTCCA
GCCATGAAACCTGAACAACAAGAAAAACTCAACTACTATGTAGAAAAAATAGCCAAAATTCTCTATCAAGAAGCTAAAGATGGCCAATTACAAAACTTAG
AAGCAATTGAAGAAACTATTAGAGCTCAAACCTTGGAGTATATCACCCCACAGATAGGAGTTTTTTTCTCAAAGAAACCACAGGAACAAGCACAGGTAGA
AGGCGATCGCTAAAAAGTATTATTGGAAATTTACCAATTACAGAAAAGCAAGCGGCGAAGTTGGATTTAAGCAATTATCAACAAATTAGTCCAAGCTTGC
AAAAGTGTTGCTTGAGAGCGAGTGCGAATGTATCGTATCAAAATGCGGCGAAAGATATCTGGGTTTATACAGGTATGAAAGTGAGTGAAAAAACCCAACA
AAGATTAGTACATAAGTATCAATTCCCAGTCGAAAACTGTGAAGCGAAAATCGCCGAAATTAGTGTCGATGGGGGGAAAGTAAGACTGAGGAGCGAAGCC
AAGGGAGTGGGTTGTACGTGGAAAGACTATAAAGCAATATGTGTCAATCAAACTCACAGAAAAGCTTGGTTTGGCGAGAACGAGCAATTAGTGACTTGGG
TAAATCAACAAGAAATGGAAGATCTGGTGACCTGTGTTGGTGATGGTCACCCGGGTATTTGGAACCTAGTATCTGGATTTGACTATCCTGGAGAAAACAG
AGAAATTCTTGACTGGTATCACTTAATAGAAAATCTACACAAGGTAGGAGGTTCCCAGAAAAGACTTAAACAAGCAGAAAATTTATTGTGGCAAGGAGAT
GTCTCAAAAACGATTGAATTGCTCAAGCCACTCAAACAAAAGCAAGCACACAATTTTGGTAATTATCTGGAAAAACATAGACACCGTATTATCAATTATA
GTTATTATCAAGCTGAGGAGATTTGTGCAATTGGCTCAGGTGCAGTGGAGTCAACTGTCAAGCAAATTGACCGAAGACTAAAAATATCGGGAGCACAATG
GAAAAAAGAAAATGTGCCCCAAGTGCTCAAACATCGATGTGCCTATCTTAATAATAGCCTTTGACTAGTACATTTGTTCTAGCAATTGTGACTTGCTCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
210 bp69 aa104313+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKPEQQEKLNYYVEKIAKILYQEAKDGQLQNLEAIEETIRAQTLEYITPQIGVFFSKKPQEQAQVEGDR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
942 bp313 aa2231164+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SSNLGVYHPTDRSFFLKETTGTSTGRRRSLKSIIGNLPITEKQAAKLDLSNYQQISPSLQKCCLRASANVSYQNAAKDIWVYTGMKVSEKTQQRLVHKYQ
FPVENCEAKIAEISVDGGKVRLRSEAKGVGCTWKDYKAICVNQTHRKAWFGENEQLVTWVNQQEMEDLVTCVGDGHPGIWNLVSGFDYPGENREILDWYH
LIENLHKVGGSQKRLKQAENLLWQGDVSKTIELLKPLKQKQAHNFGNYLEKHRHRIINYSYYQAEEICAIGSGAVESTVKQIDRRLKISGAQWKKENVPQ
VLKHRCAYLNNSL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1061 bp353 aa1041164+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKPEQQEKLNYYVEKIAKILYQEAKDGQLQNLEAIEETIRAQTLEYITPQIGVFFLKETTGTSTGRRRSLKSIIGNLPITEKQAAKLDLSNYQQISPSLQ
KCCLRASANVSYQNAAKDIWVYTGMKVSEKTQQRLVHKYQFPVENCEAKIAEISVDGGKVRLRSEAKGVGCTWKDYKAICVNQTHRKAWFGENEQLVTWV
NQQEMEDLVTCVGDGHPGIWNLVSGFDYPGENREILDWYHLIENLHKVGGSQKRLKQAENLLWQGDVSKTIELLKPLKQKQAHNFGNYLEKHRHRIINYS
YYQAEEICAIGSGAVESTVKQIDRRLKISGAQWKKENVPQVLKHRCAYLNNSL

 

Blast result :
Comments
ISGlsp1 is 81% aa similar to ISMae42.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Shih,P.M., Wu,D., Latifi,A., Axen,S.D., Fewer,D.P., Talla,E., Calteau,A., Cai,F., Tandeau de Marsac,N., Rippka,R., Herdman,M., Sivonen,K., Coursin,T., Laurent,T., Goodwin,L., Nolan,M., Davenport,K.W., Han,C.S., Rubin,E.M., Eisen,J.A., Woyke,T., Gugger,M. and Kerfeld,C.A. (2013) Proc. Natl. Acad. Sci. U.S.A. 110 (3), 1053-1058